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  • 1
    ISSN: 1600-5724
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Crystals, diffracting best to around 3 Å, have been grown from intact large and small ribosomal subunits. The bright synchrotron radiation necessary for the collection of the higher-resolution X-ray diffraction data introduces significant decay even at cryo temperatures. Nevertheless, owing to the reasonable isomorphism of the recently improved crystals of the small ribosomal subunits, reliable phases have been extracted at medium resolution (5–6 Å) and an interpretable five-derivative MIR map has been constructed. For the crystals of the large subunits, however, the situation is more complicated because at higher resolution (2.7–7 Å) they suffer from substantial radiation sensitivity, a low level of isomorphism, instability of the longest unit-cell axis and nonisotropic mosaicity. The 8 Å MIR map, constructed to gain insight into this unusual system, may provide feasible reasoning for the odd combination of the properties of these crystals as well as hints for future improvement. Parallel efforts, in which electron-microscopy-reconstructed images are being exploited for molecular-replacement studies, are also discussed.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Der Chirurg 68 (1997), S. 654-661 
    ISSN: 1433-0385
    Keywords: Key words: Assessment of risk ; Esophagectomy ; Risk score systems. ; Schlüsselwörter: Risikoerfassung ; Oesophagektomie ; Risiko-Score-System.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. Ziel der präoperativen Risikoabschätzung ist die Senkung der postoperativen Morbidität und Letalität durch eine Identifizierung gestörter Organfunktionen welche durch gezielte Maßnahmen bereits präoperativ oder durch eine problemorientierte postoperative Therapie verbessert werden können. Die präoperative Risikoerfassung nimmt damit Einfluß auf die Operationsindikation, den Operationszeitpunkt, die Verfahrenswahl und das postoperative Management. Eine notwendige Voraussetzung dafür ist es, relevante Organfunktionen mit möglichem Einfluß auf den postoperativen Verlauf zu erfassen und diese im Hinblick auf den geplanten Eingriff zu bewerten. Dieses Vorgehen gewinnt vor allem bei den heute immer umfangreicheren elektiven chirurgischen Eingriffen zunehmend an Bedeutung. In der Notfallsituation müssen dagegen die gegebenen Rahmenbedingungen in der Regel akzeptiert werden, so daß hier der präoperativen Risikoabklärung eine untergeordnete Rolle zukommt. Die objektive Risikoabschätzung erfolgt durch die Erfassung mit Bewertung von Lebensalter, Allgemein- und Ernährungszustand, pulmonale, kardiovasculäre, hepatische und Nierenfunktion, sowie Kooperationsfähigkeit des Patienten. Je nach Art und Umfang des geplanten Eingriffs kommt den einzelnen Organfunktionen eine unterschiedliche Bedeutung zu. Auf dem Boden von Basisuntersuchungen und erweiterter Diagnostik wird eine präoperative Risikoabschätzung möglich. Allgemeingültige und aus mehreren Faktoren zusammengesetzten Klassifikationssysteme zur Identifizierung von Risikogruppen haben sich mit Ausnahme einzelner und auf bestimmte Eingriffe zugeschnittene Risiko-Scores, in der klinischen Anwendung bisher jedoch nicht breit durchgesetzt. Am Modell der Oesophagektomie beim Patienten mit Oesophaguscarinom konnten wir jedoch modellhaft zeigen, daß eine quantitative Aussage über das peri- und postoperative Risiko des Patienten anhand physiologischer und präoperativ verfügbarer Parameter möglich ist und sich durch den konsequenten Einsatz eines validierten Risiko-Score-Systems die postoperative Letalität deutlich senken läßt. Die Entwicklung und Validierung ähnlicher Score-Systeme für andere Eingriffe erscheint damit wünschenswert.
    Notes: Summary. The aim of preoperative risk analysis is to reduce postoperative morbidity and mortality by identification of compromised organ function which can be improved by targeted preoperative measures and problem-oriented postoperative therapy. Ideally, therefore, preoperative risk assessment, influences the timing of the surgical procedure, the choice of the surgical approach, and the postoperative management. Identification of preexisting relevant disorders that may influence the postoperative course is an essential prerequisite of risk analysis. While preoperative risk analysis today gains in importance with the increasing extent of elective surgical procedures, preoperative risk evaluation still plays a minor role in the emergency situation when the given patient-dependent risk usually has to be accepted. Objective risk evaluation depends on the general and nutritional status, the pulmonary, cardio-vascular, hepatic, and renal function, and the cooperation of the patient. These factors, however, clearly have to be seen in relation to the type and extent of the planned surgical procedure. The selection of additional tests of organ function, exceeding the standard tests required prior to any surgical intervention, must be guided by the extent of the planned surgical procedure, the physiologic alterations associated with the surgical procedure, and the suspected underlying organ dysfunction. Generally accepted multifactorial classification systems to identify patients at risk for a wide spectrum of surgical procedures are currently not available. Using the model of esophagectomy in patients with esophageal cancer we could, however, demonstrate that a quantitative assessment of the peri- and postoperative risk based on preoperatively available physiologic parameters is possible and markedly reduces postoperative mortality when applied prospectively. The development and validation of similar risk-score systems for other surgical procedures should be considered.
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  • 3
    ISSN: 1433-0385
    Keywords: Key words: Carbohydrate-deficient transferrin ; Alcohol consumption ; Squamous cell carcinoma of the esophagus ; Preoperative risk analysis. ; Schlüsselwörter: Kohlenhydratdefizientes Transferrin (CDT) ; Alkoholkonsum ; Plattenepithelcarcinom des Oesophagus ; präoperative Risikoanalyse.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. In einer prospektiven Studie zur präoperativen Risikoerfassung der Alkoholabhängigkeit wurden 46 Patienten untersucht, bei denen eine Oesophagektomie wegen eines Plattenepithelcarcinoms durchgeführt wurde. Bei allen Patienten wurde präoperativ der Alkoholmarker kohlenhydratdefizientes Transferrin (CDT) im Serum bestimmt und mit dem Auftreten einer postoperativen Entzugssymptomatik (ja/nein) und dem postoperativen Verlauf (gut/mittel/schlecht/Tod) in Beziehung gesetzt. Für das untersuchte Kollektiv traten bei erhöhtem CDT-Wert signifikant häufiger Entzugssymptome auf (Median des CDT mit Entzug 17,0 U/l vs. ohne Entzug 10,7 U/l; p = 0,0006) bzw. fand sich bei erhöhtem CDT ein signifikant schlechterer postoperativer Verlauf (Median des CDT für mittleren/schlechten/letalen postoperativen Verlauf 14,0 U/l vs. guten Verlauf 10,8 U/l; p = 0,02). Der präoperative CDT-Wert korrelierte signifikant (p = 0,04) mit den anamnestischen Angaben über den präoperativen Alkoholkonsum (normal/erhöht/ stark erhöht). In einer multivariaten logistischen Regressionsanalyse waren CDT und präoperativer Alkoholkonsum unabhängige Parameter, die signifikant den postoperativen Verlauf bzw. Entzug vorhersagten. Für den CDT-Cut-off-Wert von 〈 15,3 U/l wurde hinsichtlich des Parameters „postoperativer Entzug eine Sensitivität“ von 71,4 % und eine Spezifität von 84,4 % berechnet. Die Bestimmung des CDT kann vor einer geplanten Oesophagektomie Patienten mit übermäßigem Alkoholkonsum sicher identifizieren.
    Notes: Summary. In a prospective study the preoperative risk of alcohol addiction was evaluated in 46 patients with squamous cell carcinoma of the esophagus. In all patients the alcohol marker carbohydrate-deficient transferrin (CDT) was measured prior to esophagectomy and correlated with the incidence of postoperative withdrawal symptoms (yes/no) and the postoperative course (good/moderate/poor/fatal). Withdrawal symptoms were more frequently observed in cases of elevated CDT values (median of CDT with withdrawal 17.0 U/l vs without withdrawal 10.7 U/l; P = 0.0006). CDT values were significantly increased in case of a complicated postoperative course (median of CDT for moderate/poor/fatal postoperative course 14.0 U/l vs good course 10.8 U/l; P = 0.02). The CDT value correlated (P = 0.04) with the patient's history of preoperative alcohol consumption (normal/increased/high). In a multivariate logistic regression analysis CDT and preoperative alcohol consumption were independent parameters to predict significantly the postoperative course and withdrawal. The sensitivity was 71.4 % and the specifity 84.4 % selecting the parameter “postoperative withdrawal” and a CDT cut-off point of 〈 15.3 U/l. CDT can effectively identify patients with high alcohol consumption prior to esophagectomy.
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  • 4
    ISSN: 1433-0474
    Keywords: Schlüsselwörter Hypoglykämie ; Insulin-abhängiger Diabetes mellitus ; cAMP ; Adrenalin ; Key words Hypoglycaemia ; Insulin-dependent diabetes mellitus ; cAMP ; Adrenaline
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Introduction and methods: The occurrence of hypoglycaemic symptoms in childhood was evaluated in 23 adolescents with insulin dependent diabetes mellitus by inducing mild hypoglycaemia in the presence of their parents. The procedure was approved by the local Ethics Committee. The objektive symptoms of hypoglycaemia were analysed by a symptom score for autonomic and neuroglycopenic symptoms of hypoglycaemia. Glucose levels were determined using a standard hexokinase method and catecholamine levels were measured radioenzymatically. A radioimmunoassay was used to estimate isoprenaline-induced cAMP levels in lymphocytes. Results: Only 34,8 % of the patients had the typical early autonomic symptoms of hypoglycaemia, whereas 65,2 % recognised neuroglycopenic symptoms of hypoglycaemia only or no symptoms at all. The occurrence of early autonomic symptoms decreased with the duration of diabetes whereas no change was found for neuroglycopenic symptoms. Only two patients with recurrent episodes of nocturnal hypoglycaemia but 9 of the parents recognised hypoglycaemia too late or not at all. Patients with recurrent episodes of anamnestic hypoglycaemia showed significantly diminished hypoglycaemia-induced adrenaline release and cAMP accumulation compared to adolescents without hypoglycaemia. Patients with neuroglycopenic symptoms of hypoglycaemia had significantly diminished cAMP accumulation compared to those with autonomic symptoms. Conclusion: Recurrent episodes of anamnestic hypoglycaemia are a main cause for frequent occurrence of hypoglycaemia unawareness in childhood diabetes mellitus. The desensitisation of adrenergic signal transduction may be an additional factor in the pathogenesis of hypoglycaemia unawareness. The parents of diabetic children and adolescents should regularly be trained with respect to the detection of hypoglycaemia symptoms.
    Notes: Zusammenfassung Fragestellung: Um die bei Kindern mit juvenilem Diabetes mellitus wenig untersuchten Erstsymptome und die adrenerge Antwort in der Hypoglykämie zu charakterisieren, führten wir bei 23 jugendlichen Diabetikern in Begleitung ihrer Eltern eine von der Ethikkommission genehmigte milde Insulin-induzierte Unterzuckerung durch. Methode: Die objektiven Unterzuckerungssymptome wurden mit Hilfe eines Symptomen-Scores für autonome und neuroglykopenische Unterzuckerungssymptome bewertet. Blutzuckerwerte wurden mit einer Hexokinasemethode und Katecholamine radioenzymatisch gemessen. Isoprenalin-stimuliertes cAMP wurde mit einem Radioimmunoassay in Lymphozyten gemessen. Ergebnisse: Nur 34,8 % der Patienten bemerkten typische autonome Erstsymptome der Unterzuckerung, 65,2 % dagegen neuroglykopenische oder überhaupt keine Erstsymptome. Je länger ihr Diabetes bestand, desto geringer waren zwar die autonomen, nicht aber die neuroglykopenischen Unterzuckerungs-Scores. Nur 2 Patienten mit nächtlichen Hypoglykämien, aber 9 Eltern bemerkten ihre Unterzuckerung zu spät oder überhaupt nicht. Patienten mit schweren anamnestischen Unterzuckerungen hatten gegenüber Patienten ohne Hypoglykämien einen signifikant verminderten Adrenalinanstieg in der Hypoglykämie und eine signifikant verminderte Stimulierbarkeit von cAMP. Patienten mit neuroglykopenischen Erstsymptomen hatten einen signifikant niedrigeren cAMP-Anstieg in der Hypoglykämie als Patienten mit autonomen Erstsymptomen. Schlußfolgerungen: Die wesentliche Ursache für die häufig fehlenden autonomen Unterzuckerungssymptome im Kindesalter sind rezidivierende Unterzuckerungen. Die Desensibilisierung der adrenergen Signalübertragung könnte einen zusätzlichen Faktor in der Pathogenese der fehlenden Unterzuckerungswahrnehmung darstellen. Eltern sollten regelmäßig bezüglich der Erkennung von Hypoglykämien ihrer Kinder geschult werden.
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  • 5
    ISSN: 1433-0474
    Keywords: Schlüsselwörter Lymphadenitis colli ; Atypische Mykobakterien (MOTT) ; Medikamentöse Therapie ; Clarithromycin ; Rifabutin ; Key words Cervical lymphadenitis ; Non tuberculous mycobacteria (MOTT) ; Drug therapy ; Clarithromycin ; Rifabutin ; Protionamid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Background: The treatment of choice of cervical lymphadenitis caused by mycobacteria other than tuberculosis is surgical. However, a complete cure can not be accomplished in every case. In selected cases a successful antimicrobial treatment has been reported. Casereport: We here report a girl aged 1 10/12 years who suffered from a cervical lymphadenitis caused by atypical mycobac-teria. Three weeks following extirpation of the affected lymphnode a new abscess de-veloped at the site of surgery. Since a complete surgical revision without affecting the facial nerve was impossible, the intervention was limited to incision and drainage of the abscess. After that, a medical treatment with rifabutin, clarythromycin, and protionamide was started. During the 6 month of therapy a complete cure could be accomplished and no severe side-effects were observed. Conclusion: In conclusion, if a surgical approach to cure a cervical lymphadenitis caused by mycobacteria other than tuberculosis is impossible for different reasons, a drug-therapy with clarithromycin, rifabutin, and protionamide seems justified and promising. The value of testing the sensitivity of mycobacteria other than tuberculosis to different drugs in planing the drug-therapy is still a matter of debate.
    Notes: Zusammenfassung Hintergrund: Die Therapie der Wahl bei Lymphadenitis colli durch atypische Mykobakterien ist chirurgisch. Nicht immer kann hiermit eine Heilung erreicht werden. Bei kompliziertem Verlauf wurde in Einzelfällen von einer erfolgreichen zusätzlichen medikamentösen Therapie berichtet. Fall: Bei einem an Lymphadenitis colli durch atypische Mykobakterien erkranktem 1 5/6jährigen Mädchen kam es 3 Wochen nach der operativen Lymphknotenexstirpation zum Rezidiv mit Abszeßbildung. Da bei einer erneuten chirurgischen Intervention der Randast des N. facialis hätte geopfert werden müssen, wurde der Abszeß nur gespalten und drainiert. Anschließend wurde eine medikamentöse Therapie mit Rifabutin, Clarithromycin und Protionamid begonnen. Während der 6monatigen Therapie wurden keine wesentlichen Nebenwirkungen beobachtet, und es kam es zur vollständigen Abheilung. Schlußfolgerung: Ist die chirurgische Therapie einer Lymphadenitis colli durch atypische Mykobakterien aufgrund ungünstiger lokaler Verhältnisse nicht möglich, ist ein medikamentöser Therapieversuch mit Clarithromycin, Rifabutin und Protionamid gerechtfertigt und erfolgversprechend. Der Stellenwert einer Empfindlichkeitsprüfung zur Planung der antimikrobiellen Therapie ist noch nicht vollständig geklärt.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of fusion energy 16 (1997), S. 37-44 
    ISSN: 1572-9591
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The decay heat-driven temperature transients of the in-vessel components following a postulated loss of all in-vessel cooling have been calculated. The resulting time-dependent heat load to the vacuum vessel is due to radiation from the backplate and convection of postulated steam between backplate and vacuum vessel. It is shown, that even for a failure of all in-vessel cooling and total loss of power, the ITER design can rely on passive decay heat removal by natural circulation in one of the two existing cooling loops of the vacuum vessel. A mathematical model describes the transient operating conditions and shows that the temperature established by natural circulation does not exceed 200°C at the maximum shut down heat load to the vacuum vessel. Therefore, no additional emergency cooling system is required if the existing heat exchanger is designed for natural circulation and a bypass is used during normal operation to maintain operation temperature.
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  • 7
    ISSN: 1432-0584
    Keywords: Key words Myelodysplastic syndromes ; Cytogenetic studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Cytogenetic analyses were performed on 266 bone marrow and peripheral blood samples from 179 patients with myelodysplastic syndromes (MDS). According to the FAB classification, 42 patients presented with RA, 18 with RARS, 37 with RAEB, 22 with CMML, and 29 with RAEB-T. Nine patients showed a secondary MDS (S MDS). FAB classification was not available for 22 patients. Clonal karyotype anomalies were found in 92 patients (51.4%). Complex chromosome abnormalities occurred in 17 (18.5%) of them. An evolution of the karyotype was detected in 16 cases (17.4%). Cytogenetically independent cells or cell clones were found in eight patients. Nonclonal chromosome abnormalities were uncovered in 29 (16.2%) of the 179 MDS patients. Consecutive studies were performed in 48 patients and revealed a good correlation of initial karyotype and clinical course. The most frequent single anomalies were 5q- in 29 (31.5%), –7 in 22 (23.9%), trisomy 1q in 14 (15.2%), and +8 in 13 (14.1%) of 92 patients respectively. Our cytogenetic findings are presented in detail and discussed in relation to patients' age, morphological classification, clinical course, and prognostic impact. The contribution of cytogenetic findings to the delineation of multistep pathogenesis of MDS with special emphasis to karyotype instability is demonstrated.
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  • 8
    ISSN: 1432-0584
    Keywords: Key words Platelet concentrates ; Buffy coat ; Platelet aphereses ; Platelet transfusion ; Corrected count increment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Random-donor platelet concentrates (PC) prepared from pooled buffy coats have recently been described as an alternative method for platelet preparation. We evaluated such PCs in the clinical setting compared with a standard PC from platelet apheresis. PCs were prepared either from pools of buffy coats (BC-PC) or from single donors (SD-PC) with the cell separator CS-3000 plus. PCs were stored for up to 5 days before transfusion. We compared fresh PC (day 1) with stored (day 2–3) and long-stored PC (day 4–5). For analysis, platelet increment in the recipient was determined immediately and 16–22 h (mean 20 h) after transfusion, corrected for total body area and transfused platelets (CCI). A total of 316 PCs were administered to 36 thrombocytopenic patients suffering from various hematological disorders. Patients with detectable HLA or platelet-specific antibodies or splenomegaly were excluded from the study. Mean platelet content of the PC was 262×109 for BC-PC and 251×109 for SD-PC. The 20-h CCI after transfusion of fresh PC was slightly higher with BC-PC than with SD-PC (14.5 versus 11.9;p=0.19), but values did not differ significantly between the two types of PC on any day of storage. For BC-PC, 20-h CCI decreased with further storage by 30% (10.2;p=0.02). For SD-PC a decrease by 9% was not significant. In conclusion, platelet concentrates prepared from pools of buffy coats showed excellent transfusion results when administered fresh, but storage decreased the CCI by 30%. No significant difference from PCs from plateletpheresis was observed on any day of storage. Both types of platelet concentrates were capable of sufficient platelet increment even when stored for up to 5 days.
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  • 9
    ISSN: 1432-0584
    Keywords: Myelodysplastic syndromes ; Cytogenetic studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Cytogenetic analyses were performed on 266 bone marrow and peripheral blood samples from 179 patients with myelodysplastic syndromes (MDS). According to the FAB classification, 42 patients presented with RA, 18 with RARS, 37 with RAEB, 22 with CMML, and 29 with RAEB-T. Nine patients showed a secondary MDS (S MDS). FAB classification was not available for 22 patients. Clonal karyotype anomalies were found in 92 patients (51.4%). Complex chromosome abnormalities occurred in 17 (18.5%) of them. An evolution of the karyotype was detected in 16 cases (17.4%). Cytogenetically independent cells or cell clones were found in eight patients. Nonclonal chromosome abnormalities were uncovered in 29 (16.2%) of the 179 MDS patients. Consecutive studies were performed in 48 patients and revealed a good correlation of initial karyotype and clinical course. The most frequent single anomalies were 5q- in 29 (31.5%), −7 in 22 (23.9%), trisomy 1q in 14 (15.2%), and +8 in 13 (14.1%) of 92 patients respectively. Our cytogenetic findings are presented in detail and discussed in relation to patients' age, morphological classification, clinical course, and prognostic impact. The contribution of cytogenetic findings to the delineation of multistep pathogenesis of MDS with special emphasis to karyotype instability is demonstrated.
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  • 10
    ISSN: 1569-8041
    Keywords: Mantle cell lymphonia ; pbpc ; therapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Mantle cell lymphoma (MC) is not curable with conventionalchemotherapy. To improve the prognosis of patients with this disease, weprospectively studied an intensive sequential therapy consisting of theDexa-BEAM regimen (dexamethasone, BCNU, etoposide, ara-C, melphalan) followedby myeloablative therapy with autologous stem cell reinfusion. Patients and methods: Nine consecutive patients with stage III/IV MC wereincluded. Two had untreated disease, four were in first remission, whereasthree had more advanced disease. All patients underwent one to two cycles ofDexa-BEAM chemotherapy to reduce the tumor load and to mobilize peripheralblood progenitor cells (PBPC). Subsequently, patients were treated withhigh-dose radiochemotherapy followed by PBPC reinfusion and were prospectivelyanalyzed for residual disease by clinical methods as well as by PCRamplification clonal CDRIII rearrangements. Results: With an overall response rate of 100%, the initialDexa-BEAM cycles effectively reduced the tumor load. All patients proceededto high-dose therapy and subsequent stem cell rescue. Engraftment was prompt,and procedure-related deaths did not occur. With a median follow-up of 12(3–33) months post transplant, all patients are alive in continuingclinical and molecular remission. Conclusions: Sequential intensive therapy consisting of Dexa-BEAM andhigh-dose radiochemotherapy appears to be a highly effective treatment forpatients with MC. However, the data are still preliminary, and larger patientnumbers and a longer follow-up are required.
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