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  • 1995-1999  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 85 (1999), S. 1372-1377 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: An argon/hydrogen (argon: 89% molar) pulse-modulated radio-frequency inductively coupled thermal plasma was generated at adequate power level (active plasma power up to about 15 kW) and has features such as control of thermal flux in time domain and introduction of nonequilibrium phenomenon. These characteristic features have ample attraction for thermal plasma materials processing. A solid state power source, which supplies the electric power at a nominal frequency of 1 MHz with high matching efficiency of 90%, was used as the pulsing generator to generate the induction plasma. Experimental measurements were carried out to analyze the transient responses of ArI (at 751 nm wavelength) line and current intensities for the imposed pulsing signal. Results were obtained for pressure range from 200 to 760 Torr, with a power on time in the range from 2 to 20 ms while the power off time ranged from 5 to 12 ms. The plate power level was varied from 11 to 17 kW though the results presented in the present article are mainly for the 11 kW (and 200 Torr) case. During the pulse modulated operation where the nonequilibrium exists more or less, the average exciting temperature of Ar atom was estimated to be varied between 5000 and 14 000 K, for pulsation of 5 ms on time with 50% duty factor, corresponding to the on and off of the pulsing signal. At different on times, ArI line intensity changed considerably; with 2 ms on time, steady-state of ICP was not achieved. For higher pressure, the intensities increased obviously and response or relaxation time was longer. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] We have identified a strong candidate cDNA for the mouse reeler gene. This 5 kb transcript encodes a 99.4 kD protein consisting of 881 amino acids and possessing two EGF–like motifs. We assayed two independent mutant alleles — ‘Jackson reeler’, which has a deletion of the ...
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Transgenic mice were produced by microinjection of a partial hepatitis C virus (HCV) genome sequence including the structural protein region, under the control of the albumin promoter and enhancer into fertilized eggs of C57BL/6 and BDF1 mice. Three founders carrying at least five copies of the transgene but not expressing HCV-specific RNA were generated. Methylation analysis indicated that the transgene was extensively methylated. Mapping of methylated cytosine residues of the transgenic mouse DNA showed that all C residues of a particular part of the HCV genome but not all the CpG island like sequences were methylated. Transiently expressed HCV cDNA in COS7 cells and the active endogenous albumin gene were not methylated. Furthermore, 5-azacytidine, a potent demethylating agent, induced HCV gene expression in a line of these transgenic mice. These results suggest that methylation of HCV cDNA is a cause of its inactive expression in transgenic mice, and that this phenomenon may occur in other stable systems for expression of the HCV genome.
    Type of Medium: Electronic Resource
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