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  • 1
    Electronic Resource
    Electronic Resource
    Effects of melatonin on macrophages/microglia in postnatal rat brain (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of pineal research 26 (1999), S. 0 
    Details
    ISSN: 1600-079X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: The present study examined the response of macrophages/microglia to multiple injections of melatonin in the pineal gland and different regions of the brain. The macrophages/microgIia showed a significant increase in cell numbers and upregulation of complement type 3 receptors (CR3), major histocompatibility complex class I (MHC I) and class II (MHC II) antigens, and antigens of monocyte/macrophage lineage, as detected by the antibodies OX-42, OX-18, OX-6, and EDJ, respectively. The upregulation of the above antigens was observed in I-d-old rats given daily injections of melatonin and killed at 7–11 d of age; no noticeable change was observed at earlier time intervals. The macrophages/microglia expressing the above antigens appeared round and showed a vacuolated cytoplasm compared with ramified cells in the control rats. Upregulation of CD4 antigens as detected with the antibody W3/25 was also observed in macrophages/microglia in the corpus callosuni and epiplexus cells in the lateral ventricles, but not in the pineal gland and the cerebral cortex in the same age group. In rats killed between 2 and 5 d, and at 14 d of age after melatonin treatment, the immunoreactivities of macrophages/microglia with the above mentioned antibodies were comparable to cells in the control rats. Immunoreactive cells were not detected in any of the age groups in melatonin-treated or control rats with the antibodies W3/13 and OX-33, which are markers for T and B lymphocytes. It is concluded that CR3 receptors, MHC antigens, and CD4 antigens on macrophages/microglia are upregulated following melatonin administration. On the other hand, once the melatonin treatment is discontinued the expression of the various antigens/receptors returns to normal levels, suggesting that increased immune potentiality and its maintenance in these cells require the continuous action of the drug.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-079X.1999.tb00578.x
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural localization of NADPH-diaphorase activity in the submucous ganglia of the guinea-pig intestine after vagotomy (1996)
    Springer
    Anatomy and embryology 193 (1996), S. 611-617 
    Details
    ISSN: 1432-0568
    Keywords: Guinea-pig ; Intestine ; Submucous ganglia ; NADPH-diaphorase ; Vagotomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The reduced nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) activity in the axon terminals presynaptic to the submucous neurons of guinea-pig intestine following unilateral cervical vagotomy was studied by electron microscopy. The reaction product of diaphorase was localized only in the axon terminals that contained predominantly small agranular vesicles, and it was usually deposited around the vesicles. The terminals that contained predominantly large granular or flattened vesicles did not display any signs of diaphorase reactivity. Although there were only few diaphorase-positive submucous neurons in the small intestine, a considerable number of diaphorase-positive axon terminals was observed in the submucous ganglia of the small intestine in the control animals. Ten days after vagotomy, the quantitative study showed that when compared with the control animals, the number of diaphorase-positive terminals in the submucous ganglia of duodenum, mid-small intestine and colon in the vagotomized animals was reduced (P〈0.05). When the NADPHd-positive terminals were examined in closer detail, it was found that only a small proportion of them showed signs of degeneration as evidenced by the swelling and vacuolation of their contents of mitochondria, with disrupted cristae and clumping of synaptic vesicles. It was therefore concluded that at least some of the diaphorasepositive axon terminals in the submucous ganglia of guinea-pig intestine originated from the vagus nerve.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00187933
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of Fos-like immunoreactivity in the brain and spinal cord of rats following middle cerebral artery occlusion (1997)
    Springer
    Experimental brain research 115 (1997), S. 129-136 
    Details
    ISSN: 1432-1106
    Keywords: Key words Fos-like immunoreactivity ; Middle cerebral artery ; Focal cerebral ischaemia ; Spinal cord neurons ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  This study examined c-fos protein expression in the brain and spinal cord of rats following permanent occlusion of the middle cerebral artery (MCA) above the rhinal fissure. At 1 h after right-sided MCA occlusion, Fos-like immunoreactivity (Fos-LI) was detected in neurons not only in the ipsilateral cerebral cortex but also in the spinal cord. In the latter, Fos-LI was localized in the nucleus and perikarya of neurons in the grey matter, notably the large motor neurons in the ventral horn. Fos-LI was most intense at 2–4 h, but became undetectable after 48 h in the cerebral cortex and 72 h in the spinal cord. In sham-operated animals, Fos-LI was almost undetectable or virtually absent. It was also not detected in the core territory supplied by the MCA at any time points after arterial occlusion. When the ischaemia-induced neuronal damage in both the cerebral cortex and spinal cord was evaluated by Nissl staining, some neurons appeared atrophic. We conclude that the induction of Fos-LI in neurons of the cerebral cortex and spinal cord is linked respectively to early onset–short stimulation and persistent excitatory or disinhibition phenomenon as a result of focal ischaemic brain injury.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00005672
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  • 4
    Electronic Resource
    Electronic Resource
    Induction of microglial and astrocytic response in the adult rat lumbar spinal cord following middle cerebral artery occlusion (1998)
    Springer
    Experimental brain research 118 (1998), S. 235-242 
    Details
    ISSN: 1432-1106
    Keywords: Key words Focal cerebral ischaemia ; Immunohistochemistry ; Microglia ; Astrocytes ; Spinal cord
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The response of microglia and astrocytes, as detected immunohistochemically by the monoclonal antibody OX-42 and anti-glial fibrillary acidic protein (GFAP) respectively, was studied in the rat lumbar spinal cord following focal cerebral ischaemia produced by permanent occlusion of the middle cerebral artery (MCA) above the rhinal fissure. At 1 and 2 days after right-sided MCA occlusion, OX-42 immunoreactivity of microglia in both the contralateral dorsal and ventral horns of the lumbar spinal cord was moderately increased compared with cells of the ipsilateral side. The microglial reaction was progressive, with some cells transformed into amoeboid form considered to be macrophages at day 3. By 5 days, many of the reactive microglia, notably in the ventral horn, appeared to encircle the soma of motoneurons. At 7 days, the microglial reaction had subsided while astrocytes in the same area were hypertrophied to replace the perineuronal microglia. The microglial response in both the cerebral cortex and lumbar spinal cord was effectively reduced by the N-methyl-d-aspartate (NMDA) receptor antagonist, MK-801. Present results suggest that following MCA occlusion, the vigorous response of microglia, and subsequently astrocytes, in the spinal cord in extra-focal areas far removed from the primary site of ischaemia may be mediated by glutamate released from the ischaemic corticospinal neurons through NMDA receptors on the postsynaptic spinal cord neurons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002210050277
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  • 5
    Electronic Resource
    Electronic Resource
    Studies of lectin receptors of rat microglia in culture: receptor distribution and internalization (1999)
    Springer
    Experimental brain research 124 (1999), S. 89-99 
    Details
    ISSN: 1432-1106
    Keywords: Key words Microglial culture ; Brain macrophages ; Isolectin ; Ultrastructure ; Intracellular pathway
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The present study examined the lectin labeling of diverse morphological forms of microglia in culture. Similar to amoeboid microglial cells in vivo, polymorphic microglia showed lectin labeling at their plasma membranes, as well as in a few cytoplasmic vesicles and vacuoles. This labeling pattern was observed in cultured microglia incubated with isolectin at 4°C for 30 min. Five minutes after the temperature was raised to 37°C, the surface lectin receptors appeared to be internalized, as shown by the occurrence of many subsurface lectin-labeled vesicles, vacuoles and tubule-like structures. With longer incubation (up to 1–2 h at 37°C), many lysosomes and a few trans-Golgi saccules and associated lysosome-like structures became labeled. Concomitant with these changes was a reduction of lectin labeling at the plasma, with labeling having vanished in most of the cells after 1–2 h of incubation. By 24 h, only a few cells retained surface lectin labeling. It appears, therefore, that irrespective of morphology, lectin labeling (including its intracellular pathway) of microglia in culture parallels that of amoeboid microglia in vivo. This would offer a useful model for the study of lectin turnover in microglia and help to explain the roles of such receptors in microglial differentiation and function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002210050603
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  • 6
    Electronic Resource
    Electronic Resource
    An ultrastructural study of cuneocerebellar neurons and primary afferent terminals in the external cuneate nucleus of gerbils as revealed by retrograde and transganglionic transport of horseradish peroxidase (1995)
    Springer
    Journal of neurocytology 24 (1995), S. 465-477 
    Details
    ISSN: 1573-7381
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The present study examined the synaptic organization of cuneocerebellar neurons and their relationships with the primary afferents in the gerbil external cuneate nucleus following multiple injections of horseradish peroxidase over a widespread area in the cerebellum in conjunction with a simultaneous injection of horseradish peroxidase into the cervical or brachial nerve plexus. The external cuneate nucleus is topographically organized: the rostral portion receiving the primary afferents from the cervical plexus and the caudal portion primary afferents from the brachial plexus. This study attempted to correlate the synaptology with the topography and different cytoarchitecture in these two specific regions in the external cuneate nucleus. Ultrastructurally, the profiles of horseradish peroxidase-labelled cuneocerebellar neurons could be divided into three types, namely, small, medium and large on the basis of their cross-sectional areas. Axon terminals which formed axosomatic synapses could be classified into: round (Rs type; 22.2%), pleomorphic (Ps type; 55.6%) and flattened (Fs type; 22.2%) vesicle boutons. The horseradish peroxidase-labelled dendritic elements of the cuneocerebellar neurons were postsynaptic to a greater number of axon terminals which were also classified into Rd (77.5%), Pd (18.8%) and Fd (3.7%) type boutons. Some of the Rd boutons making direct synaptic contacts with the cuneocerebellar neurons originated from primary afferents since they were simultaneously labelled by transganglionic transport of horseradish peroxidase. In the rostral external cuneate nucleus, synapses on cuneocerebellar neurons were more frequent on their primary dendrites as compared with those on the primary dendrites of the caudal cuneocerebellar neurons. The latter, on the other hand, showed more synapses on their distal dendrites. This may have functional implications with regard to the afferent inputs to cuneocerebellar neurons in the rostral and caudal external cuneate nucleus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01181607
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  • 7
    Electronic Resource
    Electronic Resource
    The distribution of NADPH-d in the central grey region (lamina X) of rat upper thoracic spinal cord (1995)
    Springer
    Journal of neurocytology 24 (1995), S. 735-743 
    Details
    ISSN: 1573-7381
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The distribution of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) in the central grey region (lamina X of Rexed) of the rat upper thoracic cord was examined by LM and EM. Numerous NADPH-d positive neuronal somata and fibres were present in the subependymal areas of the central grey region at levels T1–T3. Most of the neurons were located dorsal to the central canal in horizontal sections through this region. Many medially-directed NADPH-d positive fibres arising from neurons in n. intermediolateralis pars principalis, n. intercalatus spinalis and longitudinally-directed NADPH-d positive fibres arising from neurons in n. intercalatus pars paraependymalis formed a subependymal plexus. In horizontal sections through the central canal, some NADPH-d positive nerve fibres appeared to traverse the ependyma to enter and run along the central canal. By EM, NADPH-d reaction products were localized on the nuclear membrane, outer mitochondrial membrane and Golgi apparatus of both neurons and ependymal cells and in some axon terminals containing pleomorphic and round agranular synaptic vesicles. Present results suggest that besides the traditional monoamine-, amino acid- and peptidecontaining axon terminals, the central grey region also contains fibres in which nitric oxide is utilized as a neurotransmitter or neuromodulator. The finding of NADPH-d positive fibres in the central canal suggests that nitric oxide may be released into the cerebrospinal fluid. Since some of the ependymal cells were NADPH-d positive, it is suggested that they may be involved in the modulation of nitric oxide levels in the cerebrospinal fluid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01191210
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  • 8
    Electronic Resource
    Electronic Resource
    Ultrastructural identification of cholinergic neurons in the external cuneate nucleus of the gerbil: acetylcholinesterase histochemistry and choline acetyltransferase immunocytochemistry (1995)
    Springer
    Journal of neurocytology 24 (1995), S. 838-852 
    Details
    ISSN: 1573-7381
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using acetylcholinesterase histochemical and choline acetyltransferase immunocytochemical localization methods, this study has provided conclusive evidence for the existence of cholinergic neurons in the external cuneate nucleus of gerbils. By light microscopy, both acetylcholinesterase and choline acetyltransferase labelling was confined to the rostral portion of the external cuneate nucleus. Ultrastructurally, acetylcholinesterase reaction products were found in the nuclear envelope, cisternae of rough endoplasmic reticulum and Golgi saccules of some somata and large dendrites as well as in the membranes of small dendrites, myelinated axons and axon terminals. These neuronal elements were also stained for choline acetyltransferase; immunoreactivity was associated with nuclear pores, nuclear envelope, perikaryal membrane and all the membranous structures within the cytoplasm. Of the total choline acetyltransferase-labelled neuronal profiles analysed, 79% were myelinated axons, 15% dendrites, 4% somata and 2% axon terminals. The immunostained axon terminals consisted of two types containing either round (Rd type; 62.5%) or pleomorphic (Pd type; 37.5%) vesicles. Both were associated directly with choline acetyltransferase-positive dendrites. In contrast to the paucity of choline acetyltransferase-labelled axon terminals, numerous choline acetyltransferase-positive myelinated axons were present. It may thus be hypothesized that most, if not all, of the external cuneate nucleus cholinergic neurons are projection cells; such cells may give rise to axonal collaterals which synapse onto their own dendrites for possible feedback control. Choline acetyltransferase-positive dendrites were contacted by numerous unlabelled presynaptic boutons, 60% of which contained round or spherical synaptic vesicles (Rd boutons) and 40% flattened vesicles (Fd boutons), suggesting that these neurons are under strong inhibitory control. The preferential concentration of cholinergic components in the rostral external cuneate nucleus may be significant in the light of the highly organized somatotopy in the external cuneate nucleus and its extensive efferent projections to medullary autonomic-related nuclei. Our results suggest that the cholinergic neurons may be involved in somatoautonomic integration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01179983
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