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  • 1
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of CR3 complement receptors with OX-42 in amoeboid microglia in postnatal rats (1990)
    Springer
    Anatomy and embryology 182 (1990), S. 481-486 
    Details
    ISSN: 1432-0568
    Keywords: Immunocytochemistry ; Electron microscopy ; CR3 receptors ; Amoeboid microglia ; Rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The present study described the labelling of amoeboid microglial cells in the postnatal rat brain with OX-42, an antibody that recognizes type 3 complement receptors CR3 in mononuclear phagocytes. Of the diverse morphological forms of amoeboid microglia present in the corpus callosum in early postnatal (2–5 days) rats, cells with a round regular outline, or showing short stout processes, were the most intensely stained. When traced from the main cell colony into the borderline zone with the cortex, the immunoreactivity of amoeboid microglia that assumed a ramified form was drastically reduced. Examination of materials from the late postnatal (8–12 days) age group showed that the majority of the OX-42 positive cells in the corpus callosum became oval, elongated and ramified. Immunoelectron microscopy confirmed the above observations, and also showed that the immunoreactivity in the round amoeboid microglia was localized in their plasma membrane, surface projections and invaginations, as well as in some of the subsurface vacuoles. The immunoreactivity was reduced in the oval cells, and diminished in the elongated or ramified form. It is proposed that the presence of CR3 membrane receptors in amoeboid microglial cells is related to their active role in endocytosis. These, however, diminish with the growth of the brain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00178913
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of vagotomy on the ultrastructure of the atrial myocardium in the monkey (Macaca fascicularis) (1987)
    Springer
    Anatomy and embryology 177 (1987), S. 147-152 
    Details
    ISSN: 1432-0568
    Keywords: Atrial myocardium ; Vagotomy ; Monkey ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructure of the atrial myocardium in the monkey (Macaca fascicularis) was studied after bilateral cervical vagotomy and survival times of 1, 3, 5, 7, 10, 21 and 28 days. During the first week after vagotomy, a few atrial cells showed a reduction in the sarcoplasm, crowding of the myofibrils, peripheral dispersion and reduced intercristal density of the mitochondria and increased sarcoplasmic reticulum and glycogen particles. In some profiles, there was increased electron density and granularity at the I bands and the intercalated discs. The number of such affected cells increased in the subsequent days such that by 21 to 28 days about 50% of the cells were estimated to be affected. During the latter stages further changes included, the degradation of the myofilaments and increased electron density, disorganisation and disintegration of the digital extensions at the intercalated discs. Throughout the experiments there was a leucocytic infiltration, more evident in the longer survival times.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00572539
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructure of the pineal gland of the monkey, Macaca fascicularis, with special reference to the presence of synaptic junctions on pinealocytes (1989)
    Springer
    Anatomy and embryology 180 (1989), S. 151-158 
    Details
    ISSN: 1432-0568
    Keywords: Monkey ; Ultrastructure ; Pinealocytes ; Axon terminals ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The present study described the normal ultrastructure of the monkey pineal gland. The gland was composed of the principal pinealocytes, intramural neurons and glial cells. The nucleus of the pinealocytes was deeply infolded with evenly distributed chromatin materials. The abundant cytoplasm was rich in organelles including the well-developed Golgi apparatuses, multivesicular bodies, dense-cored vesicles and widely scattered free and polyribosomes. A variety of axon terminals was observed and the majority of them contained pleomorphic agranular vesicles with a few large dense-cored vesicles. A few terminals showed flattened vesicles or small dense cored vesicles. Some of the axon terminals formed synaptic contacts with the cell bodies of pinealocytes. These synapses were mainly concentrated in the posterior third of the gland. The occasional intramural neurons observed were postsynaptic to axon terminals containing round agranular vesicles. The sources of the nerve fibres and terminals forming synaptic junctions with pinealocytes and intramural neurons were discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00309766
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  • 4
    Electronic Resource
    Electronic Resource
    The ciliary ganglion of the cat: a light and electron microscopic study (1993)
    Springer
    Anatomy and embryology 187 (1993), S. 591-599 
    Details
    ISSN: 1432-0568
    Keywords: Ciliary ganglion ; Quantitative study ; Ultrastructure ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The ciliary ganglia of eight healthy adult cats were studied by light and electron microscopy. The ganglion, measuring about 2 mm in length, was consistently found to be attached to the branch from the oculomotor nerve supplying the inferior oblique muscle. The number of neurons varied from 2773 to 3794 after applying Abercrombie's correction. The mean of average somal diameter of the neurons was 36.5 μm (SD = 5.0 μm) and the mean of somal cross-sectional area was 904.2 μm2 (SD = 262.8 μm2). The mean of average nuclear diameter was 13.9 urn (SD = 1.8 μm) and the mean of nuclear cross-sectional area was 142.2 μm2 (SD = 37.1 μm2). The mean of the aspect ratios of the soma and nucleus were 1.2 (SD = 0.1) and 1.1 (SD = 0.1) respectively. The frequency distributions of these parameters were all unimodal. Under the light microscope, the Nissl granules in the neurons were prominent and were distributed peripherally, perinuclearly or randomly in the cytoplasm. Under the electron microscope, the rough endoplasmic reticulum showed a similar pattern of distribution in the cytoplasm. In some neurons, glycogen-like granules were present; these were either distributed randomly throughout the cell, or aligned in single rows in relation to sub-surface cisterns and between the cisterns of smooth and rough endoplasmic reticulum. Most of the dendrites were short protrusions from the cell body; some contained glycogen-like granules. Occasionally, the dendritic protrusions were electron-dense. All the synapses encountered were axodendritic. In most axon terminals, the synaptic vesicles were spherical and measured 30–50 nm in diameter; in some, they were flattened, measuring 50 nm by 20 nm. Some axon terminals containing either spherical or flattened synaptic vesicles also contained large dense-cored vesicles that measured 80–100 nm, while their dense core measured 40–60 nm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214438
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  • 5
    Electronic Resource
    Electronic Resource
    Ultrastructural localization of NADPH-diaphorase activity in the submucous ganglia of the guinea-pig intestine after vagotomy (1996)
    Springer
    Anatomy and embryology 193 (1996), S. 611-617 
    Details
    ISSN: 1432-0568
    Keywords: Guinea-pig ; Intestine ; Submucous ganglia ; NADPH-diaphorase ; Vagotomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The reduced nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) activity in the axon terminals presynaptic to the submucous neurons of guinea-pig intestine following unilateral cervical vagotomy was studied by electron microscopy. The reaction product of diaphorase was localized only in the axon terminals that contained predominantly small agranular vesicles, and it was usually deposited around the vesicles. The terminals that contained predominantly large granular or flattened vesicles did not display any signs of diaphorase reactivity. Although there were only few diaphorase-positive submucous neurons in the small intestine, a considerable number of diaphorase-positive axon terminals was observed in the submucous ganglia of the small intestine in the control animals. Ten days after vagotomy, the quantitative study showed that when compared with the control animals, the number of diaphorase-positive terminals in the submucous ganglia of duodenum, mid-small intestine and colon in the vagotomized animals was reduced (P〈0.05). When the NADPHd-positive terminals were examined in closer detail, it was found that only a small proportion of them showed signs of degeneration as evidenced by the swelling and vacuolation of their contents of mitochondria, with disrupted cristae and clumping of synaptic vesicles. It was therefore concluded that at least some of the diaphorasepositive axon terminals in the submucous ganglia of guinea-pig intestine originated from the vagus nerve.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00187933
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  • 6
    Electronic Resource
    Electronic Resource
    Synaptic junctions between sympathetic axon terminals and pinealocytes in the monkey Macaca fascicularis (1990)
    Springer
    Anatomy and embryology 182 (1990), S. 21-27 
    Details
    ISSN: 1432-0568
    Keywords: Monkey ; Bilateral superior cervical ganglionectomy ; Axon terminals ; Pinealocytes ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The distribution of axon terminals in the pineal gland of monkeys was studied by electron microscopy. Numerous terminals bearing small pleomorphic agranular and dense-cored vesicles were localized in the perivascular space and among the pinealocytes in the parenchyma in normal monkeys. Following bilateral superior cervical ganglionectomy, they underwent degenerative changes, including the accumulation of glycogen masses, appearance of dense residual bodies and the displacement of synaptic vesicles. Some of these degenerating terminals showed synaptic contacts with the cell bodies of pinealocytes. At the synaptic junction the postsynaptic membrane was thickened asymmetrically. Examples of synaptic contacts were most frequently observed in 5 and 7 days postoperative animals. In the longer surviving (30 days) monkey, most of the axon terminals showed round agranular vesicles, and they were mainly presynaptic to the intrapineal ganglion cells with some of the pinealocytes. They remained structurally unchanged following the resection of both the superior cervical ganglia. A few axon terminals containing small dense-cored vesicles appeared to have survived the initial insult, but some of their vesicles appeared swollen 30 days after the operation. It is concluded from this study that some of the pinealocytes are under the influence by the postganglionic neurons in the superior cervical ganglia through direct synaptic contacts. The intrapineal ganglion cells are postsynaptic to fibres originating exclusively from the central nervous system. Some of these fibres, however, may be presynaptic directly to pinealocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00187524
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  • 7
    Electronic Resource
    Electronic Resource
    A qualitative and quantitative study of substance P immuno-cytochemistry of the trigeminal ganglion in the monkey (1993)
    Springer
    Anatomy and embryology 188 (1993), S. 53-61 
    Details
    ISSN: 1432-0568
    Keywords: Monkey ; Trigeminal ganglion ; Substance P ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The immunoreactivity of substance P(SP) in the monkey trigeminal ganglion was examined and the distribution of immunoreactive cells determined. The monkey trigeminal ganglion is composed of clusters of sensory cells arranged in cords parallel to the long axis of the nerve fibres. The cells have prominent nuclei and are surrounded by satellite cells. Abundant organelles are randomly distributed throughout the cytoplasm. A striking feature of the ganglion was the presence of some axon-like prolifes containing mainly dense-cored vesicles and some agranular vesicles. Between 16 and 32% of the ganglion cells displayed SP-immunoreactivity. Most of the SP-IR cells were unipolar, small to medium-sized ganglion cells and they had no specific pattern of distribution. The staining of the SP-IR cells varied considerably, ranging from weak or moderate to heavy staining, although the majority of them were moderately stained. Immuno-electron microscopy showed that the SP-IR products were distributed throughout the soma of ganglion cells and not associated with any particular organelles or inclusions. The reaction products were also found in both myelinated and unmyelinated fibres between the ganglion cells. Another remarkable feature of the trigeminal ganglion was the occurrence of some SP-IR nerve fibres forming a rich “glomerular” network of pericellular arborizations around some of the SP-negative cells. Ultrastructural study showed the presence of some SP-IR nerve terminals in close approximation to some SP-negative cells, but there were no synaptic contacts. The relative frequency of the SP-IR pericellular arborizations paralleled the frequency of all the SP-IR cells. The results may imply that the pericellular arborizations function as a medium of communication between SP-positive and SP-negative sensory cells within the ganglion. It was suggested that the fibres forming the pericellular arborizations may originate from the intrinsic ganglion cells that are SP-positive.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00191451
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  • 8
    Electronic Resource
    Electronic Resource
    Upgrading the Activated-Sludge Process for Total Nitrogen Removal in Hong Kong (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Water and environment journal 9 (1995), S. 0 
    Details
    ISSN: 1747-6593
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: In order to protect the sensitive waters in Tolo harbour (Hong Kong), Sha Tin and Tai Po sewage-treatment works were first designed to remove 70% of the nitrogen load from the sewage. Since then, due to continuing serious eutrophication problems in the harbour, both plants have been modified to increase the removal efficiency to 90%.The modifications were based on the Bardenpho process. However, the designers of the two plants adopted different approaches to process intensity, complexity and control of bacterial foam. At Sha Tin, the average monthly results have shown an increase of total nitrogen removal from 60–70% to about 80% since its completion. The addition of methanol was found to be ineffective on further enhancement of the denitrification rate due to difficulties in the apportioning of the second anoxic zone. The overall monthly results for Tai Po have also shown an increase in the removal rate to about 80%, even though 90% was achieved for a short period of time. The major problem encountered at the latter plant was that the process design did not provide an effective control on bacterial foaming, which had affected the smooth operation of the process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1747-6593.1995.tb00929.x
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  • 9
    Electronic Resource
    Electronic Resource
    Infection of Cricket Bat Willow (Salix alba var. caerulea) Sm. by Erwinia salicis (Day) Chester detected in the Field by the Use of a Specific Antiserum (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 22 (1973), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The use of a slide agglutination test on squeezed-out sap of cultivated cricket bat willow trees allowed rapid diagnosis of infection by Erwinia salicis in the field. By this means, infection of “classically” diseased willow trees with both red leaf and wood staining was confirmed. Trees with either red leaf or wood stain, but not both symptoms, were also shown to be infected. Symptomless infection of cricket bat willow trees was detected. E. salicis was viable in the stumps of trees felled five years previously. Some of these stumps were in an advanced state of decay and some were producing symptomless leafy shoots. Trees dying with severe die-back symptoms and infected with Armil- laria mellea, were shown to be infected with E. salicis. Salix caprea and S. vitellina, two wild species of willow, were also shown to be infected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1973.tb01781.x
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  • 10
    Electronic Resource
    Electronic Resource
    A Field Survey of Watermark Disease of Cricket Bat Willow in Essex and Observations on Some of the Probable Sources of the Disease (1974)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 23 (1974), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The geographical distribution of trees of cricket bat willow (Salix alba var. caerulea) showing watermark symptoms of infection by Erwinia salicis (Day) Chester during 1955 to 1972 is recorded. In an attempt to control the disease by eradication, 23,172 trees were destroyed in this 18-year period, as were 911 mother trees (stools) producing propagating material. During an intensive survey in 1972, 301 trees and seven stools were found to be infected out of an estimated total crop of 400,000 trees. In that year, agglutination tests and culturing sap were used in addition to the examination of trees with symptoms other than watermarking in the wood. The field observations, together with some experimental inoculations, indicate that the main sources of the disease in the field each year probably are (1), the nearby stumps of felled diseased trees and (2) infected propagating material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1974.tb01814.x
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