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  • 1
    Electronic Resource
    Electronic Resource
    Strategies for promoting division of cultured plant protoplasts: synergistic beneficial effects of haemoglobin (Erythrogen) and Pluronic F-68 (1997)
    Springer
    Plant cell reports 17 (1997), S. 13-16 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsPetunia hybrida ; Oxygen delivery ; Erythrogen ; Haemoglobin ; Pluronic F-68
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Novel approaches, involving supplementation of aqueous culture medium with haemoglobin solution (Erythrogen), in the presence or absence of the copolymer surfactant, Pluronic F-68, have been evaluated to facilitate cellular oxygen availability to promote mitotic division. Cell-suspension-derived protoplasts of albino Petunia hybrida cv. Comanche were cultured for up to 45 days in KM8P medium containing 1:50–1:500 (vol:vol) Erythrogen. The mean initial protoplast plating efficiency after 9 days with 1:50 Erythrogen (18.5%) was significantly greater (P〈0.05) than in untreated controls (11.3%). Supplementation of culture medium with 1:50 Erythrogen, together with 0.01% (wt/vol) Pluronic F-68, increased the mean plating efficiency after 9 days (24.4%) by 92% (P〈0.05) over the control (12.7%). These treatments also produced increases in biomass of protoplast-derived cells up to 2.5-fold greater than control (P〈0.01) over 80 days. Gassing the medium, containing 1:50 Erythrogen, with carbon monoxide abolished the increase in plating efficiency. There was no additional benefit of gassing Erythrogen-supplemented medium with 100% oxygen. The synergistic, beneficial effect of Erythrogen and Pluronic F-68 on protoplast division has implications for plant biotechnology utilising protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050343
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  • 2
    Electronic Resource
    Electronic Resource
    Carbon dioxide-gassed fluorocarbon enhances micropropagation of rose (Rosa chinesis Jacq.) (1997)
    Springer
    Plant cell reports 17 (1997), S. 17-21 
    Details
    ISSN: 1432-203X
    Keywords: Key words Carbona dioxide ; Fluorocarbon ; Micropropagation ; Rosa chinesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The inert perfluorochemical (PFC) liquid, perfluorodecalin (Flutec PP6), has been used to increase the CO2 supply to cultured shoots of Rosa chinensis Jacq. cv. Baby Love. Culture of shoots in semi-solid medium overlaying CO2-gassed PFC (2 mbar; 5 min repeated every 7 days) for up to 42 days, increased biomass as reflected by significant (P〈0.01) increases in shoot number, number of leaves per shoot and mean shoot fresh weight. Additionally, there were significant (P〈0.01) increases in the number of roots and their fresh and dry weights following a further 10 days of culture on rooting medium prior to transfer of plants to the glasshouse. Treatment of cultured rose shoots with CO2-gassed PFC also significantly reduced (P〈0.01) the accumulation of phenolic compounds in roots. The total chlorophyll of aerial parts was unaffected, although total protein in shoots and roots was significantly (P〈0.01) lower than in the control. The biotechnological implications of this novel cultural régime are discussed for the micropropagation of woody species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050344
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A stable transformation system for the ornamental plant, Datura meteloides D. C. (1999)
    Springer
    Plant cell reports 18 (1999), S. 554-560 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAgrobacterium-mediated transformation ; Datura meteloides ; Preculture period ; Transgene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A transformation system is described for Datura meteloides using the supervirulent Agrobacterium tumefaciens strain 1065, carrying both the β-glucuronidase (gusA) and neomycin phosphotransferase II (nptII) genes between the T-DNA border sequences of the binary vector. The importance of conditions such as the preculture period of the plant tissues, wounding, bacterial dilution and incubation time were evaluated in terms of transgenic plant production. A preculture period of 2–3 days, using a 1:20 or 1:10 (vol:vol) dilution of an overnight bacterial culture, resulted in optimum shoot regeneration, with 48% from a total of 576 explants regenerating transformed shoots. Expression of the gusA and nptII genes was confirmed by a GUS fluorometric assay and by NPTII ELISA. Southern analysis revealed the integration of both transgenes, which segregated as dominant Mendelian traits in seed progeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050621
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  • 4
    Electronic Resource
    Electronic Resource
    An improved micropropagation system for Chrysanthemum based on Pluronic F-68-supplemented media (1995)
    Springer
    Plant cell, tissue and organ culture 41 (1995), S. 87-90 
    Details
    ISSN: 1573-5044
    Keywords: Dendranthema grandiflora syn. Chrysanthemum morifolium ; surfactant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Supplementation of MS-based medium containing 4.4 μM 6-benzyladenine and 2.7 μM α-naphthaleneacetic acid with 0.001–0.1% (w/v) of the non-ionic, co-polymer surfactant, Pluronic F-68, significantly (p〈0.05) increased the mean fresh weight gain of cultured leaf explants of chrysanthemum (Dendranthema grandiflora) “Tone Maid” and “Early Charm” by a maximum of 74% and 34%, respectively. The percentage of individual explants giving adventive shoots was also stimulated by Pluronic F-68; for cv. Early Charm, 0.001% (w/v) Pluronic F-68 induced the maximum response, whereas for cv. Tone Maid, the maximum response occurred with 0.01% surfactant. Shoot regeneration from explants was also enhanced at these concentrations of surfactant, compared to explants cultured in the absence of Pluronic F-68.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00051576
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  • 5
    Electronic Resource
    Electronic Resource
    An improved protocol for the culture of cassava leaf protoplasts (1995)
    Springer
    Plant cell, tissue and organ culture 42 (1995), S. 299-302 
    Details
    ISSN: 1573-5044
    Keywords: Manihot esculenta ; two-phase culture system ; glass rods ; plating efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Viable protoplasts (yield 〉 1.9 × 107 g−1 fresh weight; mean viability 85±2%, n=5) were isolated from leaves of axenic shoot cultures of Manihot esculenta Crantz. cv. M. Thai 8. Protoplasts were cultured for up to 50 days in liquid, ammonium-free MS medium, overlaying agarose-solidified B5 medium with short glass rods embedded perpendicularly within, and protruding from, the agarose layer. Control protoplasts were cultured identically, but without glass rods. Sustained protoplast division was observed only in the presence of glass rods, where the initial plating efficiency was almost 6-fold greater than control (p 〈 0.05). The mean final plating efficiency of treated cultures was 1.0±0.2% while, in contrast, significant colony formation was not observed in controls.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00030004
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  • 6
    Electronic Resource
    Electronic Resource
    Improved micropropagation of Populus spp. by Pluronic F-68 (1995)
    Springer
    Plant growth regulation 17 (1995), S. 233-239 
    Details
    ISSN: 1573-5087
    Keywords: micropropagation ; shoot regeneration ; Pluronic F-68 ; Poplar
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Stem explants and leaves (without petioles) excised from axenic shoots of Populus tremula cv. Ahle or P. tremula × tremuloides cv. Münden were cultured in the presence of the non-ionic, co-polymer surfactant, Pluronic F-68. Stem explants developed shoots within 10 d of culture and significant (p〈0.05), but genotype-dependent, increases in total shoot fresh weight (maximum 2 × control) occurred in cultures supplemented with 0.001–0.1% Pluronic F-68 over a 72 d period. Similarly, increases in both fresh weight (up to 10-fold) and number of shoots per P. tremula × tremuloides leaf explant (5-fold maximum) over 60 d occurred with Pluronic F-68 at 0.001%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024731
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