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Digitale Medien

Pre-embedding immunohistochemistry as an approach to high resolution antigen localization at the light microscopic level (1981)

Franklin, R. M. ; Martin, M. T.

Springer

Histochemistry and cell biology 72 (1981), S. 173-190

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Details

ISSN: 1432-119X

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary Pre-embedding immunohistochemistry with subsequent embedding in hydroxypropyl methacrylate enables one to obtain high resolution staining of antigens in 1 μ tissue sections. A routine method using formaldehyde fixation, methanol permeation, and an indirect method with fluoresceinlabeled second antibody is described. This method is compared with other pre-embedding staining procedures. To illustrate the method the mouse small intestine was chosen as a model and stained with antibodies to tubulin, actin, and fibronectin. Some anticipated and some unusual staining patterns were found.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00517131

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