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  • 1
    Electronic Resource
    Electronic Resource
    Guidelines for the diagnosis and interpretation of hepatic granulomas (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Histopathology 25 (1994), S. 0 
    Details
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2559.1994.tb01320.x
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  • 2
    Electronic Resource
    Electronic Resource
    Localization of thromboxane synthase in human tissues by monoclonal antibody Tü 300 (1992)
    Springer
    Virchows Archiv 421 (1992), S. 249-254 
    Details
    ISSN: 1432-2307
    Keywords: Thromboxane ; Thromboxane synthase ; Immunohistochemistry ; Mononuclear phagocyte system ; Epithelia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using the monoclonal antibody Tü 300 we localized thromboxane synthase, a secondary enzyme of the arachidonic acid cascade, employing the alkaline phosphatase anti-alkaline phosphatase method and indirect double labelling immunofluorescence in frozen sections of human tissues. Aside from platelets, the source of the antigen, all cells of the mononuclear phagocytic system were positive, including epithelioid cells and associated giant cells, starry sky macrophages, dendritic cells of T-cell areas, Langerhans cells and Kupffer cells. In addition, some epithelial cells such as epithelia of tonsillar crypts, reticular epithelia of the thymic cortex and ductular epithelia in liver, pancreas, female breast and salivary glands showed occasional focal reactivity for thromboxane synthase. We suggest that the mAb Tü 300 is a key marker for the macrophage system and the thromboxane generating system in normal and pathological conditions. It may detect functional activities of as yet unknown significance in some specialized epithelial cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01611182
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  • 3
    Electronic Resource
    Electronic Resource
    Stereology of liver biopsies from healthy volunteers (1976)
    Springer
    Virchows Archiv 371 (1976), S. 251-263 
    Details
    ISSN: 1432-2307
    Keywords: Human liver ; Electron microscopy ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The stereologioal model and the base-line data of normal human liver needle biopsy-specimens are presented. Four reference systems were introduced: 1 cm3 of liver tissue, 1 cm3 of hepatoeyte, 1 cm3 of hepatocytic cytoplasm and the volume of an average “mononuclear” hepatocyte. The sampling was done at three levels of magnification (1,000 ×, 5,000 × and 10,000 ×). A lobular differentiation was not considered. The baseline data show strikingly small variations (s.e. less than 10%) within the individual biopsy specimen and within the group of four biopsies. There is no principal difference between human beings presented here, rats, mice and dogs. Only the mean individual volume of human hepatocytes is clearly larger than in rodents. The problems and limitations of stereological work on liver biopsy specimens are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00433072
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  • 4
    Electronic Resource
    Electronic Resource
    p53 anderbB-2 protein overexpression are associated with early invasion and metastasis in bladder cancer (1993)
    Springer
    Virchows Archiv 423 (1993), S. 329-334 
    Details
    ISSN: 1432-2307
    Keywords: Bladder neoplasm ; p53 ; erbB-2 ; Metastasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Overexpression of p53 anderbB-2 was studied by immunohistochemistry in formalin-fixed tissue samples of 179 patients with transitional cell carcinoma of the urinary bladder. p53 immunostaining was strongly correlated with tumour stage (P〈0.0001). This was driven by a marked difference in p53 expression between pTa (37% positive) and pT1 (71%) tumours, while there was no difference between pT1 and pT2-4 tumours. Similarly, a strong overall association between p53 expression and grade (P〈0.0001) was driven by a marked difference between grade 1 (28%) and grade 2 tumours (71%), and there was no significant difference between grade 2 and grade 3 tumours. Surprisingly, the frequency oferbB-2 overexpression was higher in pT1 tumours (74%) than in either pTa (49%;P=0.0265) or pT2-T4 (56%;P=0.0645) tumours. Both p53 anderbB-2 expression was also associated with metastasis. Metastases were found in 77% of patients with p53 positive primary tumours, but in only 50% of the patients with p53 negative primary tumours (P=0.022). Metastases were found in 66% of patients witherbB-2 positive primaries, but in only 37% of theerbB-2 negative primaries (P=0.020). Of 32 patients with positivity for both p53 anderbB-2, 84% developed metastases, as compared to 49% of patients with positivity for either one or neither positive (P=0.002). We conclude that both p53 anderbB-2 over-expression are associated with early invasion in bladder cancer. Furthermore, p53 anderbB-2 may be important predictors for metastasis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01607144
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  • 5
    Electronic Resource
    Electronic Resource
    Diagnostic tools for differentiating pleural mesothelioma from lung adenocarcinoma in paraffin embedded tissue (1993)
    Springer
    Virchows Archiv 423 (1993), S. 493-496 
    Details
    ISSN: 1432-2307
    Keywords: Malignant mesothelioma ; Lung adenocarcinoma ; Immunohistochemistry ; Expert system ; Discriminant analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A panel of 14 antibodies (panepithelial antibody Lu-5, anti-keratin-18, anti-keratin-7, Ber-EP4, anti-Leu-M1, HEA-125, anti-carcinoembryonic antigen, anti-blood group-related antigens A, B, H, B72.3, antiplacental alkaline phosphatase, anti-vimentin and BMA-120), which have been evaluated for use in differentiating mesothelioma from lung adenocarcinoma, was applied to a group of 24 suspected mesotheliomas. Using the established qualitative, descriptive criteria derived from monovariate statistical analysis of the tumour control groups (definite mesotheliomas, adenocarcinomas), a definitive allocation was possible in only 25% of suspected cases. We therefore constructed two “expert systems”, based on multivariate discriminant analysis with either the ALLOC 80 program for ordinal data or a newly developed analysis program for binomial data. With these two systems diagnostic allocation of suspected mesotheliomas was improved to 75% and 79%. The use of binomial data (“positive” versus “negative”) in conjunction with the probability-based test system is of particular interest because the primary data are easy to record and the test results have a higher statistical probability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01606540
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  • 6
    Electronic Resource
    Electronic Resource
    Diagnostic tools for differentiating between pleural mesothelioma and lung adenocarcinoma in paraffin embedded tissue. Part I: immunohistochemical findings (1993)
    Springer
    Virchows Archiv 423 (1993), S. 19-27 
    Details
    ISSN: 1432-2307
    Keywords: Malignant mesothelioma ; Lung adenocarcinoma ; Ber-EP4 ; BMA-120 ; Blood-group-isoantigen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Specimens of 27 histologically definite mesotheliomas and 34 proven adenocarcinomas were examined with a panel of 14 antibodies: pan-epithelial antibody Lu-5, anti-keratin-18, anti-keratin-7, Ber-EP4, anti-Leu-M1, HEA-125, anti-carcino-embryonic antigen (CEA), anti-blood group-related antigens (anti-BGR A, B, H), B 72.3, anti-placental alkaline phosphatase (PLAP), anti-vimentin and BMA-120 used to determine their value in the differentiation between pleural mesothelioma and lung adenocarcinoma. Lu-5, anti-cytokeratin-7 and -18, B 72.3 and PLAP reacted in a high percentage of cases with both mesothelioma and adenocarcinoma. Anti-CEA and anti-Leu-Ml did not react with any of the 27 mesotheliomas tested but showed a reaction in 75% (anti-CEA) and 66% (anti-Leu-M1) of the lung adenocarcinomas. Seventeen percent of the adenocarcinomas and 96% of the mesotheliomas showed a positive reaction with anti-vimentin. Ber-EP4 was demonstrated in all lung adenocarcinomas, but only in 2 mesotheliomas in a focal manner (7%). HEA-125 and anti-BGR A, B, H reacted with 83% (HEA-125) and 75% (anti-BGR A, B, H) of the lung adenocarcinomas. The statistical parameters, sensitivity and efficiency were estimated and a normogram for judging the diagnostic power of a single antibody for the differential diagnosis of mesothelioma versus adenocarcinoma was developed. According to this, Ber-EP4, HEA-125, anti-BGR A, B, H and anti-CEA were, in descending order, the most powerful discriminatory antibodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01606427
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  • 7
    Electronic Resource
    Electronic Resource
    Cardiac myxomas: Immunohistochemical study of benign and malignant variants (1991)
    Springer
    Virchows Archiv 418 (1991), S. 485-491 
    Details
    ISSN: 1432-2307
    Keywords: Cardiac myxoma ; Immunohistochemistry ; Histogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Immunohistochemical investigation of 11 cardiac myxomas (CMs) including one malignant metastasizing CM showed a co-expression of epithelial (lu-5 and CAM 5.2), mesenchymal (vimentin) and neuroendocrine antigens (neuron-specific enolase) in all tumour cells. Factor VIII was found in the endothelial cells of capillaries only. In the subendocardium of fetal heart tissue close to the foramen ovale myofibroblasts reacting with the panepithelial antibody lu-5 were detected. We conclude that CMs are neoplasms that may develop from embryonic cell remnants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01606497
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  • 8
    Electronic Resource
    Electronic Resource
    The localization of thromboxane synthase in normal and pathological human kidney tissue using a monoclonal antibody Tü 300 (1994)
    Springer
    Virchows Archiv 424 (1994), S. 69-74 
    Details
    ISSN: 1432-2307
    Keywords: Kidney ; Thromboxane ; Thromboxane synthase ; Tü 300 monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Thromboxane, excreted in the urine in increased amounts in glomerular, vascular and tubulo-interstitial diseases, is considered to originate from the kidney. The localization of thromboxane synthase, a key enzyme of arachidonic acid metabolism, was studied in the human kidney by immunohistology using the monoclonal antibody Tü 300. In the interstitial tissue dendritic reticulum cells surrounding the tubules expressed high concentrations of the enzyme. In glomeruli the enzyme was weakly expressed in podocytes. This was confirmed by co-localization with an antiserum directed to podocalyxin, a marker of the visceral epithelial cells. In the study of various kidney diseases, massive accumulation of thromboxane synthase containing cells was observed in interstitial diseases, whereas in glomerular diseases there were no differences from normal kidney; in a case of thrombotic microangiopathy podocytes exhibited an increase in thromboxane-synthase. The thromboxane-synthase positive infiltrating interstitial cells were shown by conventional light microscopy to be mononuclear phagocytic cells. The physiological sources of renal thromboxane are dendritic reticular cells and podocytes. In interstitial renal disease infiltrating cells of the monocyte/macrophage system constitute the major site of thromboxane synthesis. In glomerular disease, a characteristic alteration of thromboxane-synthase was not found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197395
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  • 9
    Electronic Resource
    Electronic Resource
    Long-term cultivation of plasma cell leukemia cells and autologous lymphoblasts (LCL) in vitro: A comparative study (1978)
    Springer
    Annals of hematology 36 (1978), S. 331-338 
    Details
    ISSN: 1432-0584
    Keywords: Plasma cell leukemia ; LCL ; EBV
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two long-term cell lines were established in vitro from the peripheral blood of a patient with plasma cell leukemia: one line with plasma cell proliferation, the other with lymphoblastoid cell proliferation (LCL). The 9-month-old plasma cell line showed the typical morphology of plasmoblasts. The cells neither had B-nor T-lymphocyte characteristics, were EBV negative, and showed aneuploidy with various marker chromosomes, including the 14 q+ marker. The cytogenetic findings indicate a monoclonal proliferation of the plasmacells. No tumor growth in thymusless nude mice could be induced upon intracranial inoculation with these cells. In contrast, the autologous LCL, cultured after addition of exogenous EBV, showed the characteristic markers of lymphoblastoid cells, with the typical morphology of pear and handmirror-shaped lymphoblasts, growing in clumps. They had C3- and Fc-receptors, surface-Ig, E-rosette-negativity, a diploid karyotype, and EBV dependent macromolecule synthesis. The lymphoblastoid cells produced intracranial tumors in nude mice in 8 out of 8 attempts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01000590
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  • 10
    Electronic Resource
    Electronic Resource
    Pathological cell aggregates in bone marrow cultures from patients with various hematological diseases (1979)
    Springer
    Annals of hematology 38 (1979), S. 457-465 
    Details
    ISSN: 1432-0584
    Keywords: Nicht-klonale Zellaggregate ; Methylzellulosekulturen ; Makrophagen ; Immunreaktion in vitro ; Non-clonal cell aggregates ; Human bone marrow cultures ; Macrophages ; Immune response in vitro
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Non-clonal growth of macroscopic cell aggregates in methylcellulose cultures of abnormal marrow is described. They were seen in all patients with Graft-versus-Host Disease, graft rejection, and autoimmune disease presumably directed against hemopoietic cells, we found them in 35% of patients with primary hematological neoplasias and rarely in patients with solid tumors. They were never encountered in 80 healthy controls. The aggregates originated from small cell clumps which sedimented with the “buffy coat” in contrast to normal bone marrow particles. They contained tumor cells, grafted myeloid cells, or target cells of autoimmune disease in association with a widely varying amount of macrophages. Preliminary results suggest that the frequency of macrophages within the aggregates correlates inversely with the aggressiveness of the clinical condition. We propose that appearance of such aggregates is an indicator of immune activation; we expect that further quantitation of the phenomenon will reveal important clinical correlations and provide a model for the study of host defense to “foreign” cells.
    Notes: Zusammenfassung Das Wachstum abnormer, nicht-klonaler Zellaggregate in Methylzellulose-Kulturen wird beschrieben. Diese wurden beobachtet in Kulturen von Patienten mit Graft-versus-Host-Krankheit, in der Absto\ungsphase nach Knochenmarkstransplantation, bei autoimmun-hämolytischer Anämie sowie bei Patienten mit malignen Hämoblastosen. In 80 Kulturen von Gesunden wurden sie nicht gesehen. Sie entstanden aus abnormen Knochenmarksbröckeln, welche — im Unterschied zu normalen Bröckeln — mit der „Speckhaut“ sedimentierten. Sie enthielten Tumorzellen, transplantierte hämopoetische Zellen oder Erythroblasten bei autoimmun-hämolytischer Anämie, welche von einer unterschiedlichen Zahl Makrophagen umgeben waren. Erste Resultate lassen vermuten, da\ der Grad dieser Makrophagenreaktion bei Krankheiten mit klinisch günstigem Verlauf stärker ist als bei sehr malignen Krankheiten. Wir interpretieren das Phänomen als Ausdruck einer aktivierten Immunitätslage und schlagen vor, da\ es als in vitro-Modell für das Studium menschlicher Immunreaktionen verwendet werden kann.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01013506
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