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  • 1990-1994  (1)
  • 1975-1979  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Magnesium distribution in human bone (1994)
    Springer
    Calcified tissue international 54 (1994), S. 34-37 
    Details
    ISSN: 1432-0827
    Keywords: Magnesium ; Bone ; Aging ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract The present study was undertaken to reveal the magnesium distribution in human bone. Sixty human ribs, obtained from subjects aged 10–80 years of age, were used. Transverse sections were prepared from the middle region of the human ribs. Adjacent sections were ground to a thickness of about 1000 μm. One section was used for magnesium determination by atomic absorption spectrophotometry, and the other was used for analysis with X-ray microanalysis. Thirty micron thick samples were abraded continuously from the periosteal and the endosteal surfaces by abrasive microsampling, as previously described by Weatherell et al. [3]. Results showed that magnesium concentrations were higher in both the periosteal and endosteal surfaces and did not change with age in general, although it tended to be higher among teenagers and lower over 80 years old.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00316287
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Variation in susceptibility of HeLa cell lines to coxsackievirus A 9 (1979)
    Springer
    Archives of virology 59 (1979), S. 213-222 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the course of serial passages for several years a line of uncloned HeLa cells (A line) showed a gradual decrease in plaquing efficiency by coxsackievirus A 9 (CA 9 virus), while subcultures prepared from the same line kept frozen at an early passage level (A original line) did not show any change. However, it was observed later that the plaque-forming ability of the A original line (A orig. line) also decreased after serial passages as was observed with the A line. Comparing the characteristics of the same cell line at two different passage levels, it was found that the efficiency of adsorption of virus to cells was nearly the same, while virus yield at 8 hours after infection was different. The activity of alkaline phosphatase of cells was also different between these two passage levels, suggesting that a high enzymatic activity is associated with the susceptibility of cell cultures to CA 9 virus. Magnesium chloride at 25 mM enhanced plaque formation by CA 9 virus in highly passaged less susceptible cell cultures, and a possible role of the chemical as a stabilizer of alkaline phosphatase was discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01317416
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    Paper (German National Licenses)
    Fulltext
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