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  • 1990-1994  (6)
  • Immunohistochemistry  (2)
  • Middle ear  (2)
  • Nodose ganglion  (1)
  • microsurgery  (1)
  • (Rat liver)
Datenquelle
Materialart
Erscheinungszeitraum
Jahr
Schlagwörter
  • 1
    Digitale Medien
    Digitale Medien
    Frameless isocentric stereotactic laser beam guide for image-directed microsurgery (1993)
    Springer
    Acta neurochirurgica 125 (1993), S. 177-180 
    Details
    ISSN: 0942-0940
    Schlagwort(e): Laser guide ; microsurgery ; navigation ; stereotaxy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary We have developed a frameless stereotactic multi-articulated arm suitable for image-directed microsurgery and surgical navigation. The system comprises a frameless isocentric mechanism and a three-dimensional (3 D) digitizer for intra-operative monitoring. Five joints of our multi-articulated arm are equipped with electromagnetic clutches, and the arms are provided with counterbalancing weights to ensure safe, smooth movement. Stereotactic microsurgical procedures were carried out with a laser beam guide mounted at the tip of the isocentric arm. We have used this system in 22 cases to resect tumours or epileptic foci. There were 12 cases of glioma, 5 cases of deep seated benign tumours, 3 cases of multiple metastatic tumours, and 2 cases of temporal lobe epilepsy. Postoperative enhanced CT images indicated total or subtotal resection in all cases. We also have used this system to determine the approach for clipping of a posterior cerebral artery aneurysm in 2 cases. The error of mechanical accuracy of the system was less than 1 mm. We believe that this system will prove useful in image-directed microsurgical procedures.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01401848
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  • 2
    Digitale Medien
    Digitale Medien
    Distribution of tyrosine hydroxylase immunoreactive nerve fibers in the canine larynx (1992)
    Springer
    European archives of oto-rhino-laryngology and head & neck 249 (1992), S. 40-43 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Immunohistochemistry ; Tyrosine hydroxylase ; Sympathetic nerve ; Larynx-Dog
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The sympathetic innervation of the canine larynx was investigated using tyrosine hydroxylase (TH) immunohistochemistry. Many tyrosine hydroxylase immunoreactive (TH-IR) nerve fibers were observed around arteries and arterioles in the laryngeal mucosa and intrinsic laryngeal muscles. In the glandular region, TH-IR fibers were also found, with some of these fibers terminating around the basement membranes of the glandular cells. The quantity of TH-IR fibers in the mucosa differed among regions of the larynx. Many of these fibers could be found in the laryngeal surface of the epiglottis as well as the posterior glottis. These findings suggest that TH-IR fibers may directly innervate muscles in the intrinsic larynx.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00175669
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  • 3
    Digitale Medien
    Digitale Medien
    Distribution of calcitonin gene-related peptide nerve fibers in the canine larynx (1992)
    Springer
    European archives of oto-rhino-laryngology and head & neck 249 (1992), S. 52-55 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Calcitonin gene-related peptide ; Canine larynx ; Immunohistochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Immunohistochemistry was used to investigate the distribution pattern of calcitonin gene-related peptide (CGRP) nerve fibers in the laryngeal mucosa, glands and intrinsic muscles of the dog. CGRP immunoreactive nerve fibers were found more frequently than substance P immunoreative nerve fibers in every region of the larynx. In the epithelia, CGRP nerve fibers were mainly found in the epiglottis, arytenoid region and subglottis. Many taste buds were observed in the arytenoid region and were densely innervated by the CGRP nerve fibers. In the lamina propria, the plexus of CGRP nerve fibers was present, with some of these fibers associated with blood vessels. Laryngeal glands were also innervated by a few CGRP nerve fibers. In the intrinsic laryngeal muscles, abundant immunoreactivity was observed and many motor end-plate-like structures were found with CGRP immunoreactivity. These findings strongly suggest that CGRP plays an important role in all of the sensory, motor and autonomic nervous systems of the larynx.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00175672
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  • 4
    Digitale Medien
    Digitale Medien
    Sugar-binding sites with specificity for glucosamine in the guinea pig middle ear mucosa (1993)
    Springer
    European archives of oto-rhino-laryngology and head & neck 250 (1993), S. 412-417 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Sugar-binding site ; Guinea pig ; Middle ear ; Lipopolysaccharide ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Glucosamine-binding sites were detected in Lowicryl K4M-embedded guinea pig middle ear mucosa by electron microscopy, using glucosaminyl bovine serum albumin. Incubation of ultrathin tissue sections with gold-labeled glucosaminyl bovine serum albumin (GlcN/BSA/gold) resulted in binding mainly on cilia, microvilli, rough endoplasmic reticulum and nuclei. The sugar binding was not inhibited after ultrathin sections had been digested with trypsin or neuraminidase. Various carbohydrates and glycoconjugates were tested as competitive inhibitors of G1cN/BSA/gold labeling on the tissue sections. The sugar specificity range detected by the glucosamine-binding sites included glucosamine, N-acetylglucosamine, mannose and fucose, whereas N-acetylgalactosamine, galactose and glucose were not detectable. A series of endotoxic substances such as Salmonella minnesota Re595 lipid A complex with BSA and lipopolysaccharides (LPS) derived from Escherichia coli 055: B5 or S. minnesota Re595 also competed with GlcN/BSA/gold binding. This indicates that the lipid A backbone glucosamine or other carbohydrate portions of LPS is a part of the structure recognized by glucosamine binding sites.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00180388
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  • 5
    Digitale Medien
    Digitale Medien
    Localization of the sensory neurons in the canine nodose ganglion sending fibers into the internal branch of the superior laryngeal nerve (1991)
    Springer
    European archives of oto-rhino-laryngology and head & neck 248 (1991), S. 265-267 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Superior laryngeal nerve ; Nodose ganglion ; Larynx ; Horseradish peroxidase ; Dog
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The distinct localization of the sensory neurons in the canine nodose ganglion that send fibers into the internal branch of the superior laryngeal nerve (the main sensory pathway from the larynx) was studied using horseradish peroxidase technique. The labeled cells were located mainly in the rostral one-third of the lateral side of the nodose ganglion. We conclude that the localization of these labeled cells does not reflect a difference in cellular role or function but is due to the course of the nerve's fibers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00176751
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  • 6
    Digitale Medien
    Digitale Medien
    Glycoconjugate histochemistry in the glycocalyx of guinea pig middle ear mucosa (1990)
    Springer
    European archives of oto-rhino-laryngology and head & neck 247 (1990), S. 291-295 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Middle ear ; Lectin ; Glycocalyx
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary An attempt was made to determine the composition of oligosaccharide side chains in the apical cell surface glycocalices of the middle ear epithelium. This glycoconjugate is located on the innermost luminal surface, but its function is still unknown. The lectins evaluated included Triticum vulgare, Canavalia ensiformis, Maclura pomifera, Arachis hypogaea, Ricinus communis I (RCA-I), and Limax flavus. The staining results obtained with the six different lectin probes in the Lowicryl K4M-embedded guinea pig middle ear mucosa suggest that the glycocalyx contains β-d-N-acetylglucosamine, sialic acid, and β-d-galactose in the major carbohydrate moiety. The present study also indicates that a polarity exists on the cell surfaces in the distribution pattern of the carbohydrate component, especially those of β-galactosyl residues, as revealed by RCA-I binding. This may imply functional separation of the epithelial cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00176540
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