ISSN:
1433-2981
Keywords:
Abdominal aorta
;
Bleeding site
;
Coagulation
;
Haematology
;
Inferior vena cava
;
Orbital venous plexus
;
Plasma chemistry
;
Rats
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Of paramount importance to most toxicity studies in rats is the evaluation of haematological, coagulation and clinical chemistry parameters. In European and North American countries, the orbital venous plexus (OVP) is currently the most common route for obtaining blood, whereas in Japan the inferior vena cava (IVC) and abdominal aorta (AA) are the preferred routes. In order to compare clinical pathology parameters from the three bleeding sites, 11-week-old male F344 rats were anaesthetised by ether inhalation, and blood samples collected by IVC, AA or OVP for subsequent haematological, coagulation and plasma chemistry analysis. Collection methods utilised a plastic (5 ml) syringe and needle for IVC and AA, and glass capillary tubes (1.5 mm × 30 mm) for OVP. Ten haematology parameters, two coagulation and 22 plasma chemistry parameters were assayed, and the results compared between the three bleeding sites. The results showed there were no essential differences in the haematological or plasma chemistry values when blood was withdrawn from either the IVC or OVP. However, blood collected from the AA exhibited white cell counts of only 40%\2-60% of the values from the other two sites, and plasma glucose values showed slightly higher values. Other haematological, coagulation and plasma chemistry values showed no meaningful differences between the three bleeding sites, any differences being small and not considered to be biologically or clinically significant. Although some values may vary with the selection of bleeding site, careful and gentle sampling, avoiding stress and artefacts (e.g. tissue fluid) will minimise these differences. It is important therefore that clinical pathology assays during the course of a toxicity study should use the same sample site, preanalytical conditions and analytical methods. The results obtained in this study will be used as baseline data for haematology and clinical chemistry characteristics for the three bleeding sites in male F344 rats.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00185175
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