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  • 1990-1994  (3)
  • Ausdauerbelastung  (1)
  • Organic Chemistry  (1)
  • clinical HIV-1 isolates  (1)
  • 1
    ISSN: 1573-8280
    Keywords: Neutralizing monoclonal antibody ; principal neutralizing determinant ; clinical HIV-1 isolates ; anti-idiotypic antibody ; gp120 ELISA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The pharmacokinetics of mouse V/human C (γ1,κ) chimeric monoclonal antibody CGP47 439 specific for the principal neutralizing determinant of human immunodeficiency virus type 1 (HIV-1) was studied in patients with stage IV HIV-1 disease in an open-labeled phase I/IIA trial. Twelve male patients were enrolled and nine completed the study. Patients were divided into three groups according to the extent of CGP 47 439 to bind to gp120 from their viral isolates: undetectable for group 1, modestly reactive for group 2, and strongly reactive for group 3. A first dose of 1, 10, or 25 mg was administered by intravenous infusion to group 1, group 2 and group 3 patients, respectively. The patients then received seven doses of 50, 100, or 200 mg, respectively, every three weeks. CGP 47 439 serum concentrations were determined by an ELISA using monoclonal antibody AB19-4 specific for the idiotope of CGP 47 439. Half an hour after infusion only 25.5–36.1% of the administered antibody was found in the serum, reflecting its rapid distribution in the extravascular space and possibly binding to gp120 antigen in some of the patients. The terminal elimination half-life (T1/2) was 16.2 days in group 1 patients, 9.7 days in group 2 and in group 3 patients 7.5 days and 9.1 days. An antibody response to CGP 47 439 was not a factor in determining elimination rates, because only very low and transient responses were found in three patients. These results suggest that the reactivity of CGP 47 439 with HIV-1 gp120 contributed to its elimination in HIV-1 infected patients.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1436-6215
    Keywords: Ausdauerbelastung ; Sportgetränke ; Kohlenhydrataufnahme ; Energieumsatz ; Stoffwechsel ; “post-exercise ketosis” ; endurance exercise ; sports beverages ; carbohydrate consumption ; energy turnover ; metabolism ; post-exercise ketosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary The present study addressed the effects of carbohydrate consumption during endurance exercise on performance, energy turnover, and metabolism. Well-trained endurance runners consumed a beverage with (cho[+]) or without (cho[−]) carbohydrates during a long-distance run (46.6 km). The respiratory quotient (RQ), plasma levels of carbohydrate and fat metabolites, and of hormones (insulin, glucagon) were measured before, several times during, and after the run. The mean running speed for the entire distance was 13.6 and 13.4 km/h with the cho[+] and cho[−] beverage, respectively. The decrease in speed that was observed towards the end of the run was somewhat more pronounced with consumption of the cho[−] beverage. The RQ decreased during the run almost linearily. This decrease was independent of the consumed beverage. The changes in plasma levels of lactate, free fatty acids (FFA), glycerol, D-3-hydroxybutyrate (DHB), glucagon and insulin that occurred during the run were not affected by intake of the cho[+] beverage. However, intake of the cho [+] beverage prevented the decrease in plasma glucose observed towards the end of the run under control conditions, and eliminated the steep postexercise increase in plasma DHB. The intake of the cho[+] beverage also caused a rapid decrease in plasma levels of FFA and glucagon after the run, and slightly increased plasma insulin. The results demonstrate that ingestion of a carbohydrate-containing beverage during a long-distance run affects metabolism only during the final phase of the run and during the subsequent recovery period. Moreover, carbohydrate consumption improves performance only during the final phase of a long-distance run.
    Notes: Zusammenfassung In der vorliegenden Studie wurde der Einfluß der Kohlenhydrataufnahme während eines Langstreckenlaufes über 46,6 km auf Leistungsfähigkeit, Energieumsatz und Stoffwechsel untersucht. Gut trainierte Läufer erhielten während des Laufes entweder ein kohlenhydrathaltiges (KH[+]) oder ein kohlenhydratfreies (KH[−]) Getränk. Der respiratorische Quotient (RQ), die Plasmakonzentrationen von Metaboliten des Kohlenhydrat- bzw. Fettstoffwechsels und von Hormonen (Insulin, Glucagon) wurden gemessen. Die mittlere Geschwindigkeit über die gesamte Distanz betrug 13,6 (KH[+]) bzw. 13,4 (KH[−]) km/h. Dabei war der gegen Ende des Laufes allgemein feststellbare Leistungsabfall bei Aufnahme des KH[+]-Getränkes etwas schwächer ausgeprägt als bei Aufnahme des KH[−]-Getränkes. Der RQ nahm unabhängig von der Kohlenhydrataufnahme während des gesamten Laufes annähernd linear ab. Die während des Laufes feststellbaren Veränderungen der Plasmakonzentrationen von Lactat, Freien Fettsäuren (FFS), Glycerin, D-3-Hydroxybutyrat (DHB), Glucagon und Insulin wurden durch die Kohlenhydrataufnahme nicht signifikant beeinflußt. Die Aufnahme des KH[+]-Getränkes verhinderte jedoch den unter Kontrollbedingungen gegen Ende des Laufes feststellbaren Abfall der Plasmaglucosekonzentration sowie den steilen Anstieg des Plasmaspiegels von DHB nach dem Lauf. Ferner führte die Kohlenhydrataufnahme zu einem raschen Abfall der Plasmakonzentrationen von FFS und Glucagon nach dem Lauf und erhöhte die Plasmakonzentration von Insulin geringfügig. Die Ergebnisse zeigen, daß die exogene Zufuhr von Kohlenhydraten den Stoffwechsel erst in der Endphase eines Langstreckenlaufes sowie in der anschließenden Erholungsphase beeinflußt. Ein positiver Effekt der Kohlenhydrataufnahme auf die Leistungsfähigkeit tritt ebenfalls erst in der Endphase eines so langen Laufes auf.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0941-1216
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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