ISSN:
1432-1106
Keywords:
Ca2+- and phospholipid-dependent protein kinase
;
Visual cortex
;
EM-immunocytochemistry
;
Rat
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary In the primary visual cortex of adult rats the cellular and subcellular distribution of protein kinase C isozymes II and III (PkCII/III) was examined by immunohistochemical methods with a monoclonal antibody against PkCII/III. Strong PkC(II/III)-immunoreactivity was found in neurons and astrocytes. Immunopositive neurons exhibited morphological features characteristic for both pyramidal and non-pyramidal cells. They were distributed in layers II through VI but were concentrated in layers II/III. At the electron microscopic level immunoprecipitate was found predominantly in distinct regions of the somata, except the nuclei, and only a few labeled dendrites and axons were seen. Two different patterns of cytoplasmic immunoreactivity could be distinguished. In most neurons, PkC(II/III)-staining was confined to cytoplasmic spots associated with the Golgi complex, while a few neurons exhibited additional labeling in the vicinity of the cell membrane. Moreover, PkC(II/III)-immunoreactivity was present in numerous astroglial processes and in the perikaryal cytoplasm of a subpopulation of astrocytes. Present data provide morphological indications for specifie functions of PkC isozymes II and III in neurons as well as in astrocytes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00228798
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