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  • 1990-1994  (9)
  • Immunocytochemistry  (5)
  • Myocardial ischemia  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Effects of prostacyclin analogue, OP-2507, on function and metabolism in the ischemic working rat heart (1992)
    Springer
    Journal of anesthesia 6 (1992), S. 446-454 
    Details
    ISSN: 1438-8359
    Keywords: Myocardial ischemia ; Myocardial metabolism ; Prostacyclin analogue OP-2507 ; Working rat heart preparation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We examined the effects of a new stable prostacyclin analogue, OP-2507, on myocardial function and metabolism in the ischemic working rat heart preparation. The hearts were perfused with Krebs-Henseleit bicarbonate (KHB) buffer, and whole heart ischemia was induced by one-way aortic valve for 15 min follows by reperfusion for 30 min. In the treated hearts, OP-2507, 20 ng·ml−1, was administered to KHB buffer from the beginning to the end of experiment. During ischemia, coronary flow in the OP-2507 group increased significantly more than that in the control group. The mechanical performance of both groups was impaired after ischemia. However, the recovery of coronary flow, cardiac output, peak systolic pressure and LV dP/dTmax was significantly higher in the treated group than in the control group. The incidence of ventricular fibrillation during reperfusion was 100% and 25% in the control and the OP-2507 groups, respectively. Myocardial ATP content was significantly higher in the treated hearts than that in the control hearts. These results indicate that this stable prostacyclin analogue is beneficial in myocardial ischemia, even without its well known action of preventing platelet aggregation. (Oguchi T, Kashimoto S, Nakamura T, et al.: Effects of prostacyclin analogue, OP-2507, on function and metabolism in the ischemic working rat heart. J Anesth 6: 446–454, 1992)
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s0054020060446
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  • 2
    Electronic Resource
    Electronic Resource
    Bundles of amyloid precursor protein-immunoreactive axons in human cerebrovascular white matter lesions (1994)
    Springer
    Acta neuropathologica 87 (1994), S. 450-455 
    Details
    ISSN: 1432-0533
    Keywords: Amyloid precursor protein Chromogranin A ; Synaptophysin ; White matter lesions ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Cerebral white matter lesions commonly observed in Binswanger's disease, multi-infarct encephalopathy and elderly people are neuropathologically characterized by diffuse incomplete demyelination and considered to be ischemic in nature. Arteriolosclerosis in the white matter is a common feature in these white matter lesions. To investigate a possible alteration of the distribution of amyloid precursor protein (APP), chromogranin A (CgA) and synaptophysin (Syn) in such white matter lesions, we examined 15 cases with white matter lesions and 5 without white matter lesions. Many bundles of axons with APP-like immunoreactivity (LI) were observed particularly in mild white matter lesions. Such bundles of axons showed similar but less intense CgA-LI and Syn-LI. They appeared to occur in areas with many ameboid or ramified microglia labeled with anti-leukocyte common antigen and few astrocytes labeled with anti-glial fibrillary acidic protein. In the center of moderate of severe white matter lesions bundles of axons with APP-LI were never observed. Since APP, CgA and Syn undergo fast axonal transport, and since following ischemic insults to central nervous system microglial reaction occurs earlier than astroglial changes, our results suggest that axonal damage, which induces disturbance of fast axonal transport, can occur even in the early stage of white matter lesions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294171
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  • 3
    Electronic Resource
    Electronic Resource
    Ubiquitin-immunoreactive inclusions in anterior horn cells and hypoglossal neurons in a case with Joseph's disease (1993)
    Springer
    Acta neuropathologica 85 (1993), S. 341-344 
    Details
    ISSN: 1432-0533
    Keywords: Joseph's disease ; Ubiquitin ; Immunocytochemistry ; Anterior horn cell ; Hypoglossal nucleus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We describe a patient with progressive spastic ataxia and ophthalmoparesis. His clinical and neuropathological findings were consistent with Joseph's disease. The most characteristic neuropathological features in the present case were ubiquitin-immunoreactive filamentous or dense inclusions in spinal anterior horn cells and hypoglossal neurons, which have been considered to be a specific finding in amyotrophic lateral sclerosis (ALS). The occurrence of ubiquitin-immunoreactive inclusions suggests that such inclusions are not totally specific to ALS and could occur in occasional degenerating motor neurons without apparent ALS neuropathology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227732
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  • 4
    Electronic Resource
    Electronic Resource
    Immunocytochemical identification of androgen receptor in mouse osteoclast-like multinucleated cells (1994)
    Springer
    Calcified tissue international 54 (1994), S. 325-326 
    Details
    ISSN: 1432-0827
    Keywords: Androgen Receptor ; Osteoclast ; Mouse ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Expression of androgen receptor (AR) in mouse osteoclast-like multi-nucleated cells (OCs) was examined with immunocytochemical techniques. Murine OCs were obtained by co-culturing mouse osteoblastic cells and bone marrow cells. Three preparations of polyclonal anti-AR antibody which were raised in rabbit against different parts of the human AR were employed for the experiments. Specific staining for AR was demonstrated in the nuclei and the perinuclear area of mouse OCs. This is the first report demonstrating the presence of AR in osteoclast-like cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00295958
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  • 5
    Electronic Resource
    Electronic Resource
    Immunolocalization of 67 kDa elastin-binding protein in perinatal rat lungs (1992)
    Springer
    Cell & tissue research 268 (1992), S. 277-281 
    Details
    ISSN: 1432-0878
    Keywords: Lung ; Elastin-binding protein ; Lectins ; Galactose ; Monocytes ; Immunocytochemistry ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 67 kDa elastin-binding protein (RL-67EBP) has been isolated from neonatal rat lungs by the use of an elastin-coupled affinity column, followed by elution with either lactose or synthetic elastin hexapeptide (VGVAPG), and immunohistochemistry has been used on perinatal rat lungs to determine the tissue localization of this protein. No immunoreactive structures occur in fetal lungs, or in the lungs of day-1 and-4 neonates. On day-7 after birth, immunoreactive cells appear in the subepithelial connective tissue of the intrapulmonary airways, from day-10 on, these cells become evenly distributed in the alveolar parenchyma. Occasionally, some cells occur in the alveolar air space, being free from the surface of the alveolar septum. Unpermeabilized cells obtained by bronchoalveolar lavage, show cell surface immunoreactivity, indicating that RL-67EBP is expressed on the surface membrane of the cells. From these findings, it is suggested that the immunoreactive cells are blood-borne monocytes, and that RL-67EBP may function as an elastin peptide receptor by which monocytes mobilize through interstitial connective tissue during their migration from blood to alveolar air space, where they eventually differentiate into alveolar macrophages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318796
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  • 6
    Electronic Resource
    Electronic Resource
    Changes in myocardial nonesterified fatty acids during ischemia and reperfusion in isolated, perfused, working rat hearts (1990)
    Springer
    Heart and vessels 6 (1990), S. 21-30 
    Details
    ISSN: 1615-2573
    Keywords: Myocardial ischemia ; Nonesterified fatty acids ; Lactate ; High energy phosphate ; Isolated rat heart
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The time course of changes in the myocardial levels of nonesterified fatty acids (NEFA), adenosine triphosphate (ATP), creatine phosphate (CrP) and lactate, and those in the cardiac mechanical function during ischemia and reperfusion was investigated in the isolated, perfused, working rat heart. Ischemia was produced by lowering the afterload pressure from 60 to 0 mm Hg, and reperfusion resulted from raising the afterload pressure to 60 mm Hg. Ischemia stopped the heart beat, and increased the myocardial levels of unsaturated NEFA (such as arachidonic, palmitoleic, and linoleic acids) as a function of the ischemic period; it decreased the myocardial levels of ATP and CrP, and increased the myocardial level of lactate. The level of arachidonic acid increased when the myocardial level of ATP fell below 5 µmol/g dry weight. Reperfusion after ischemia started the heart beat, and restored the mechanical function which depended on the preceding ischemic period. Reperfusion also increased the levels of ATP and CrP and decreased the level of lactate, whereas it further increased the levels of the NEFA that had been elevated by ischemia. The recovery of mechanical function was inversely correlated with the myocardial level of arachidonic acid during ischemia and reperfusion. We concluded that changes in the myocardial levels of NEFA during ischemia and reperfusion are different from those of ATP, CrP, and lactate, and suggest that the myocardial level of arachidonic acid during ischemia and reperfusion can be a sensitive and suitable marker for the recovery of mechanical function during reperfusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02301877
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  • 7
    Electronic Resource
    Electronic Resource
    Effects of endothelin-1 on epicardial coronary tone, coronary blood flow, ECG-ST change and regional wall motion in anesthetized dogs (1991)
    Springer
    Heart and vessels 6 (1991), S. 191-196 
    Details
    ISSN: 1615-2573
    Keywords: Endothelin-1 ; Myocardial ischemia ; Epicardial coronary artery diameter ; Coronary blood flow ; Regional wall motion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effects of intracoronaryadministrated endothelin-1 on coronary hemodynamics and regional myocardial function were studied in anesthetized open-chest dogs. Epicardial coronary diameter (CoD) and coronary blood flow (CBF) were measured by a sonomicrometer of 10 MHz piezoelectric crystals and an electromagnetic flow probe on the left circumflex coronary artery (LCX). Regional wall motion was sonomicrometrically measured at regions supplied by the LCX and left anterior descending artery (LAD) and electrocardiograms were recorded. Endothelin-1, administered as a bolus injections into the LCX via an intracoronary cannula, in a dose-dependent manner reduced COD and CBF. The extent of the reduction of COD and CBF at a dose of 300 pmol was 12.3±1.5% (P〈0.01) and 86±5% (p〈0.01), respectively, of the control. The extent of CBF reduction and deterioration of systolic wall motion were linearly related with the dosage of endothelin-1. ST-elevation (lead II) and fatal ECG abnormalities, including complete atrioventricular block or ventricular fibrillation, were observed with doses above 60 and 100 pmol, respectively. Coronary angiography revealed that filling defects of dye were propagated from the third or distal branches to those of more proximal arteries when the doses of endothelin-1 were cumulatively infused into the LCX. Accordingly, lethal myocardial ischemia induced by endothelin-1 is produced by critical obstruction of rather small coronary vessels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02125096
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  • 8
    Electronic Resource
    Electronic Resource
    Effects of propafenone on function and metabolism in the ischemic working rat heart (1992)
    Springer
    Heart and vessels 7 (1992), S. 169-174 
    Details
    ISSN: 1615-2573
    Keywords: Myocardial ischemia ; Myocardial metabolism ; Propafenone ; Working rat heart preparation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We examined the effects of propafenone, a new anti-dysrhythmic agent, on myocardial function and metabolism in ischemic working rat heart preparations. In the treated hearts, propafenone, 0.3 µg/ml and 3 µg/ml, were added to Krebs-Henseleit bicarbonate buffer perfusate throughout the experiments. Whole heart ischemia was induced through a one-way aortic valve for 15 min followed by reperfusion for 30 min. After induction of ischemia, the cardiac output, peak aortic systolic pressure, left ventricular dP/dtmax, and myocardial ATP concentration were greater in the treated hearts than in the untreated ones. All hearts in the untreated group developed ventricular fibrillation (Vf) at the beginning of the reperfusion period. On the other hand, no treated hearts had Vf at any time during the experiment. However, propafenone, 3 µg/ml, evoked a negative inotropic effect before and after ischemia. These results indicate that propafenone may contribute to early recovery from ischemia in myocardial function and metabolism, although it has a negative inotropic action.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01744601
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  • 9
    Electronic Resource
    Electronic Resource
    Translocation of enamel proteins from inner enamel epithelia to odontoblasts during mouse tooth development (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 238 (1994), S. 383-396 
    Details
    ISSN: 0003-276X
    Keywords: Tooth development ; Mouse ; Protein translocation ; Amelogenin ; Epithelial-mesenchymal interactions ; Intercellular communication ; Immunocytochemistry ; Differential gene expression ; In vitro organ culture ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The developmental problem of how dental epithelia and/or dental papilla ectomesenchyme induce and/or up- or down-regulate tooth formation are as yet unresolved issues. We have desinged studies to map the synthesis and fate pathways of secreted amelogenin proteins from Kallenbach differentiation zones II-IV during in vivo and in vitro mouse mandibular first molar tooth development (M1). Tooth organs from cap, bell, and crown stages were processed for reverse transcriptase/polymerase chain reaction (RT-PCR) and high resolution Protein A immunocytochemistry using anti-amelogenin and anti-peptide antibodies. Cap stage M1 were cultured for periods ranging from 10-21 days in vitro using either serumless, or 15% fetal calf sera-supplemented, chemically-defined medium. Amelogenin transcripts are expressed in the mouse embryonic molar from E15 through early postnatal development. Amelogenin antigens were first detected in Kallenbach's differentiation zone II. Amelogenin proteins secreted from preameloblasts were identified along cell processes and cell surfaces of odontoblasts adjacent to forming mantle dentine extracellular matrix (ECM) prior to biomineralization. Amelogenin proteins were restricted to forming endocytotic vesicles, clathrin-coated vesicles, and lysozomes within odontoblasts. At later stages (e.g. 2 days postnatal development), enamel proteins were not identified in odontoblasts or predentine matrix following mineralization. Comparable observations for stages of development were noted for in vitro cultured tooth explants. Preameloblasts synthesize and secrete amelogenin proteins which bind to odontoblast cell surfaces possibly through the process of receptor-mediated endocytosis. We conclude that amelogenin proteins secreted from preameloblasts, prior to the initiation of biomineralization, were translocated to odontoblasts to serve as yet unknown biological functions. © 1994 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092380313
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