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  • 1
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 75 (1994), S. 5444-5446 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Nanocrystalline thin SnO2 semiconductor films (thickness≤1 μm) have been modified with chlorophyll b (Chl-b) by electrodeposition and adsorption methods for use as novel photosensitive electrodes in photoelectrochemical cells. Excitation of Chl-b with monochromatic light produced photocurrents with an incident photon-to-photocurrent efficiency of around 8.5%. The charge injection from excited Chl-b into the conduction band of the semiconductor SnO2 crystallites has been probed by time-resolved microwave absorption.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 40 (1994), S. 263-268 
    ISSN: 1573-5079
    Keywords: photoacoustie spectroscopy ; Photosystem I ; electron transport ; thermal dissipation ; plastoquinone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photoacoustic spectroscopy was used to study the thermal deactivation processes in a Photosystem I submembrane fraction isolated from spinach. A large part of the thermal dissipation was variable. The yield of this variable thermal emission depended on the redox state of the Photosystem. It increased with the measuring modulated light intensity coinciding with the gradual closure of the reaction centers. Thermal deactivation was maximal when the reaction centers were closed by a saturating illumination. Extrapolation of the data at zero light intensity indicated that the yield of non-variable thermal emission represented about 37% of the maximal emission. The presence of methylviologen as artificial electron acceptor decreased the yield of variable thermal emission whereas inhibition following heat stress treatments increased it. The significance of the variable and non-variable components of thermal dissipation is discussed and the measured energy storage is suggested to originate from the reduction of the plastoquinone pool during cyclic electron transport around Photosystem I.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5079
    Keywords: atrazine ; calcium ; chloride ; depleted-photosystem II ; DCIP-photoreduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The oxygen evolving complex of photosystem II (PS II) contains three extrinsic polypeptides of approximate molecular weights 16, 23 and 33 kDa. These polypeptides are associated with the roles of Cl-, Ca2+ and Mn2+ in oxygen evolution. We have shown that selective removal of 16 and 23 kDa polypeptides from the above complex by NaCl washing of PS II enriched membrane fragments renders the PS II core complex more susceptible to the herbicide atrazine. On the other hand, when both native and depleted preparations were resupplied with exogenous Ca2+ and Cl-, we obtained a reduction of atrazine inhibition which was much stronger in the depleted preparations than in the native ones. It is concluded that removal of 16 and 23 kDa polypeptides in general, and disorganization of associated Ca2+ and Cl- in particular, enhances atrazine penetration to its sites of action in the vicinity of the PS II complex. The above could be interpreted if we assume a reduced plastoquinone affinity at the QB (secondary plastoquinone electron acceptor) pocket of D1 polypeptide following transmembranous modifications caused by the depletion of these polypeptides.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5079
    Keywords: photosynthesis ; thermal emission ; P700 ; quantum yield ; energy conversion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thermal emission and photochemical energy storage were examined in photosystem I reaction center/core antenna complexes (about 40 Chl a/P700) using photoacoustic spectroscopy. Satisfactory signals could only be obtained from samples bound to hydroxyapatite and all samples had a low signal-to-noise ratio compared to either PS I or PS II in thylakoid membranes. The energy storage signal was saturated at low intensity (half saturation at 1.5 W m-2) and predicted a photochemical quantum yield of 〉90%. Exogenous donors and acceptors had no effect on the signal amplitudes indicating that energy storage is the result of charge separation between endogenous components. Fe(CN)6 -3 oxidation of P700 and dithionite-induced reduction of acceptors FA-FB inhibited energy storage. These data are compatible with the hypothesis that energy storage in PS I arises from charge separation between P700 and Fe-S centers FA-FB that is stable on the time scale of the photoacoustic modulation. High intensity background light (160 W m-2) caused an irreversible loss of energy storage and correlated with a decrease in oxidizable P700; both are probably the result of high light-induced photoinhibition. By analogy to the low fluorescence yield of PS I, the low signal-to-noise ratio in these preparations is attributed to the short lifetime of Chl singlet excited states in PS I-40 and its indirect effect on the yield of thermal emission.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5079
    Keywords: electron transport ; oxygen uptake ; Photosystem I ; superoxide radicals ; superoxide dismutase ; heat stress ; electron donors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A Photosystem I submembrane fraction isolated from spinach was used to study the mechanism of heat-stress stimulation of oxygen uptake by the photosystem. Various artificial electron donors were shown to generate electron transport reactions with various degrees of thermally induced stimulation. A strong stimulation was observed with durohydroquinone as electron donor with a maximal effect at 50 °C. The degree of stimulation obtained was independent from the redox potential of the electron donors and from their oxidation site because the enzyme superoxide dismutase fully inhibited the stimulation. Instead, it is proposed that thermal stress causes the release of membrane bound superoxide dismutase from the thylakoids thus allowing the reduced form of electron donors with specific properties to reduce O2 − radicals to H2O2 besides the usual disproportionation of O2 − into O2 and H2O2.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 23 (1990), S. 313-318 
    ISSN: 1573-5079
    Keywords: thylakoid membranes ; electron transfer ; photoacoustic spectroscopy ; energy storage ; photosynthesis ; plastoquinone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The photosynthetic energy storage yield of uncoupled thylakoid membranes was monitored by photoacoustic spectroscopy at various measuring beam intensities. The energy storage rate as evaluated by the half-saturation measuring beam intensity (i50) was inhibited by 3-(3,4-dichlorophenyl)-1,1 dimethylurea, by heat inactivation or by artificial electron acceptors specific for photosystem I or photosystem II; and was activated by electron donors to photosystem I. The reactions involving both photosystems were all characterized by a similar maximal energy storage yield of 16±2 percent. The data could be interpreted if we assumed that the energy storage elicited by the photosystems at 35 Hz is detected at the level of the plastoquinone pool.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 30 (1991), S. 123-130 
    ISSN: 1573-5079
    Keywords: oxygen evolution ; calcium ; chloride ; electron transport ; photosynthetic inhibitors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The inhibitory effect of Zn2+ on photosynthetic electron transport was investigated in native and CaCl2-treated (depleted in extrinsic polypeptides) Photosystem II (PS II) submembrane preparations. Inhibition of 2,6-dichlorophenolindophenol photoreduction by Zn2+ was much stronger in protein-depleted preparations in comparison to the native form. It was found that Ca2+ significantly reduced the inhibition in the native PS II preparations, as did Mn2+ in a combination with H2O2 in the protein-depleted counterparts. No other tested monovalent or divalent cations could replace Ca2+ or Mn2+ in the respective experiments. Diphenylcarbazide could partially relieve (40–45%) the inhibition in both types of preparations. The above indicates the presence of an active Zn2+ inhibitory site on the donor side of PS II. However, neither Ca2+ nor Mn2+ could completely prevent inhibition by high concentrations of Zn2+ (〉1 mM). We propose that elevated levels of Zn2+ strongly perturb the conformation of the PS II core complex and might also affect the acceptor side of the photosystem.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 44 (1994), S. 178-183 
    ISSN: 0006-3592
    Keywords: bioassay ; phytotoxicity ; photosynthesis ; immobilized membranes ; oxygen evolution ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The potential of thylakoid membranes immobilized in an albumin-glutaraldehyde crosslinked matrix in a fast bioassay for phytotoxicity measurements in aqueous samples is studied. Free and immobilized preparations are compared for their electron transport activity measured as the initial rate of oxygen evolution with 2,5-cichlorobenzoquinone as the artificial electron acceptor. Immobilized thylakoids were much stable under storage conditions; in the dark, at 4°C, they were fully stable in terms of photosynthetic activity for a period of 200 h. The immobilized membranes were as sensitive as the free thylakoids for the detection of most of the compounds tested (metal cations, sulfite, nitrite, and herbicides), all known as inhibitors of photosynthetic electron transport. In some instances, the immobilized preparations were even more sensitive than the free counterparts. The sensitivity could be further increased by lowering chlorophyll concentration in the assay. The short incubation period required (∼10 to 15 min) and the small volume of the assay (3 mL) suggest that this type of material should be useful in the detection of locations or effluents with phytotoxic character. © 1994 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Biotechnology techniques 4 (1990), S. 363-368 
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Thylakoid membranes isolated from spinach leaves were used as the biological sensing material to elaborate a biosensor for the detection of small amount of the herbicides atrazine and diuron. Free and immobilized thylakoid membranes were compared for their responses to inhibition by herbicides by following the variation of the photocurrent. Immobilized thylakoid membranes were twice as sensitive to inhibition by herbicides than the native thylakoids.
    Type of Medium: Electronic Resource
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