ISSN:
1573-5079
Keywords:
oxygen evolution
;
calcium
;
chloride
;
electron transport
;
photosynthetic inhibitors
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The inhibitory effect of Zn2+ on photosynthetic electron transport was investigated in native and CaCl2-treated (depleted in extrinsic polypeptides) Photosystem II (PS II) submembrane preparations. Inhibition of 2,6-dichlorophenolindophenol photoreduction by Zn2+ was much stronger in protein-depleted preparations in comparison to the native form. It was found that Ca2+ significantly reduced the inhibition in the native PS II preparations, as did Mn2+ in a combination with H2O2 in the protein-depleted counterparts. No other tested monovalent or divalent cations could replace Ca2+ or Mn2+ in the respective experiments. Diphenylcarbazide could partially relieve (40–45%) the inhibition in both types of preparations. The above indicates the presence of an active Zn2+ inhibitory site on the donor side of PS II. However, neither Ca2+ nor Mn2+ could completely prevent inhibition by high concentrations of Zn2+ (〉1 mM). We propose that elevated levels of Zn2+ strongly perturb the conformation of the PS II core complex and might also affect the acceptor side of the photosystem.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00042010
