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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 20 (1990), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: There is an increasing interest in measuring human plasma histamine levels in various clinical conditions. A variety of ‘old’ and newly developed techniques are applied to meet this demand. However, the discrepancy between reported reference values for histamine in human plasma measured using this variety of techniques, suggests the existence of a certain degree of inaccuracy and imprecision. We therefore organized an external quality control study on the reliability of current histamine determinations in European laboratories. Three lyophilized plasma quality control samples, in duplicate, covering the normal and pathological range of histamine concentrations (0–45 nmol/l), two different aqueous histamine standard samples and one solvent sample were sent to 10 laboratories for the analysis of their histamine content. The following methods were used: gas chromatography-mass spectrometry (n= 2), enzymatic single isotopic assay (n= 1), fluorometric-fluoroenzymatic assay (n= 3), radioimmunoassay (n= 3) and high performance liquid chromatography (n= 2). The study was performed and evaluated according to the approved recommendations (1983) of the International Federation of Clinical Chemistry (IFCC). The target values ±s.d. of the three plasma samples were: 39·5±4·6 nmol/1 (CV=ll·6%), 2·3 ± 2·2 nmol/1 (CV = 96%) and 8·9±1·5 nmol/1 (CV = 17%), respectively. The target values ±s.d. of the two aqueous samples were: 0·9±1·1 nmol/1 (CV = 120%; true value: 0·00 nmol/1) and 10·2 ± 0·5 nmol/1 (CV = 5·3%; true value: 10·0 nmol/1), respectively. A Youden plot of two unrelated plasma samples in the pathological range defined 7/11 results as accurate and precise. The Youden plot of the two unrelated aqueous samples only denned 6/11 results as accurate and precise. In general, estimating histamine concentrations within the normal range seemed to be the most difficult part of measuring histamine in human plasma samples. It is suggested to define reference standards, methods and laboratories for plasma histamine determinations.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We have studied the hadronic production of charmed mesons in the NA 32 experiment at CERN. A special trigger together with a high resolution vertex detector consisting of charge coupled devices and silicon microstrip detectors allowed the selection of very clean samples of charmed mesons. We have collected 852 fully reconstructed decays: 60D s + →K + K −π+, 543D°→K −π+ andK −π+π−π+ as well as 249D +→K −π+π+ (or charge conjugate). 147 mesons out of our $${{D^0 } \mathord{\left/ {\vphantom {{D^0 } {\bar D^0 }}} \right. \kern-\nulldelimiterspace} {\bar D^0 }}$$ sample were produced via chargedD * state. For all charmed mesons we determine the total production cross-section and study thex F andp t 2 distributions.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Der Anaesthesist 43 (1994), S. 786-790 
    ISSN: 1432-055X
    Keywords: Schlüsselwörter: Trauma – Kardiopulmonale Reanimation – Notarztsysteme ; Key words: Trauma – Cardiopulmonary resuscitation – Emergency medical systems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract. Posttraumatic cardiopulmonary resuscitation (CPR) is associated with a poor outcome. When evaluating the literature according to the Utstein method, there were only 2 survivors (0.18%) out of 1,135 CPR attempts after trauma (Table 1). Differences in the study populations and levels of prehospital trauma care led us to analyse the results of a physician-staffed prehospital trauma care system in Cologne. Methods. From January 1987 to December 1990, a total of 49,054 emergency calls were registered using a standardised protocol. Among 9,595 trauma-related calls, 636 patients were found to be pulseless on arrival of the emergency team, 412 of these were pronounced dead. CPR was initiated in the remaining 224 patients, who comprise the study population (defined as 100%). All patients who were admitted to a hospital were followed using a second protocol. Results. CPR in the field was successful in 68 (30.4%) patients, who were then admitted to a hospital; 42 of these died within the first 24 h. Four patients (1.8%) could be discharged from hospital alive and were still living 1 year later, 1 with a lasting neurological deficit (Fig. 1). In 156 (69.6%) cases resuscitative attempts were unsuccessful in the field. Conclusions. Even in a physician-staffed prehospital trauma care system, the chance of surviving a post-traumatic cardiac arrest is minimal. Survival has to be regarded as an individual fate; the overall results are discouraging. Even though this study analyses the largest population of posttraumatic CPR ever published, prognostic factors could not be identified due to the few survivors. Nevertheless, the result does not justify general omission of CPR after trauma as: (1) prognostic factors for survival have not been identified thus far; and (2) no significant additional costs arise from posttraumatic CPR.
    Notes: Zusammenfassung. Für die präklinische kardiopulmonale Reanimation nach Trauma werden in der Literatur Überlebensraten zwischen 0% und 1,7% beschrieben. Die vorliegende Arbeit analysiert die Ergebnisse der Reanimation Schwerverletzter am Beispiel einer Millionenstadt mit einem organisierten Notarztdienst. Vom 01. 01. 1987 bis zum 31. 12. 1990 wurden vom Kölner Rettungsdienst 9595 Verletzte präklinisch behandelt. Von diesen waren 636 bei Ankunft des Notarztes pulslos, in 224 Fällen (definiert als 100%) wurde eine kardiopulmonale Reanimation begonnen. 68 (30,4%) Patienten konnten in ein Krankenhaus eingeliefert werden, vier (1,8%) wurden lebend entlassen. Ein Schwerverletzter mit Herz-Kreislaufstillstand hat eine nur minimale Überlebenschance. Auch bei maximaler präklinischer Therapie unter Einsatz eines Notarztes ist die Überlebensquote enttäuschend. Trotzdem darf nicht grundsätzlich auf eine Reanimation Schwerverletzter verzichtet werden, weil keine Prognosefaktoren zur Identifizierung potentiell Überlebender existieren und weil keine zusätzlichen signifikanten Kosten entstehen.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The increasing importance of measuring histamine in many clinical conditions and the variety of currently used techniques enforced us to organize an external quality control study (ring study) on the reliability of histamine measurements in European laboratories. Three plasma quality control samples in duplicate (lyophilized) with different amounts of histamine (0–5 ng/ml), two different aqueous histamine standard samples and one solvent sample were sent to 10 laboratories for analysis of their histamine content. The following methods were used: gas chromatographic-massespectrometric technique (n=2), single isotope assay (n=1), fluorometric-fluoroenzymatic assay (n=3), radioimmunoassay (n=3), HPLC technique (n=2). The study was performed and evaluated according to the approved recommendation (1983) of the International Federation of Clinical Chemistry (IFCC). This first report of the study is concentrated on the imprecision and inaccuracy of the different principal methods and laboratories by comparing two unrelated plasma histamine samples of different analyte concentrations (target values and SD: 4.39±0.51 ng/ml and 0.99±0.17 ng/ml). This study showed a fairly good agreement between most participants. 7/11 results obtained with 4 different methods were accurate and precise in the plasma range of histamine (Youden plot). Results outside the borderlines turned out to be a problem of the analyst rather than the method itself. It is suggested to define reference values for plasma histamine and to establish reference laboratories and methods according to the IFCC-guidelines.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Inflammation research 30 (1990), S. 271-273 
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In a prospective clinical trial, plasma histamine levels were measured in 28 polytrauma patients on day 1, 5 and 14 after trauma. Only those subjects who died were drop-outs. All patients had severe polytrauma with at least 3 body regions involved. The median plasma histamine levels at all three time points were significantly higher than in patients with single trauma of the extremities or before selective orthopaedic surgery but still in the normal range (〈1 ng/ml). However, all patients with plasma levels above 1 ng/ml on days 1 and 5 died, as did all patients with levels above 0.5 ng/ml on day 1. Thus the elevation of plasma histamine levels, for whatever reason, appears to be a prognostic factor for bad outcome in polytrauma patients.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Surgical endoscopy and other interventional techniques 7 (1993), S. 482-488 
    ISSN: 1432-2218
    Keywords: Pain ; Postoperative ; Local anesthetic ; Laparoscopic cholecystectomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The aim of this study was to investigate whether local anesthesia of abdominal wall wounds prior to laparoscopic cholecystectomy leads to decreased pain beyond the immediate postoperative period and thus improves the comfort of the patient. In a randomized, double-blind study 50 patients scheduled for laparoscopic cholecystectomy were divided into two groups. In one group (n=25) the skin, subcutis, fascia, muscle, and preperitoneal space were infiltrated with 8 ml of bupivacaine 0.5% 5 min before each abdominal wall incision. The control group (n=25) received normal saline. The intensity of pain was assessed by a 100-point visual analogue scale (VAS) at rest and during movement and by the consumption of analgesics. Analgesic therapy was provided by on-demand analgesia with piritramid intravenously for 24 h and continued by ibuprofen orally on request. The mean intensity of pain at rest and during movement was lower but not statistically significant in patients who received bupivacaine compared to the control group up to the second postoperative day. The difference was between 4 and 9 VAS points and therefore of doubtful clinical relevance. Similar statistically nonsignificant results were found for the mean consumption of piritramid up to 16 h after the operation. Three patients (12%) in the bupivacaine group localized the most severe pain up to the second postoperative day to the right lower abdominal wall wound where the gallbladder had been extracted compared to 11 patients (44%) of the control group (P=0.012). These results indicate that bupivacaine was effective at the site where it was administered. However, preincisional local anesthesia of the abdominal wall wounds in laparoscopic cholecystectomy does not lead to a significant clinical benefit for the patient.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Surgical endoscopy and other interventional techniques 8 (1994), S. 90-96 
    ISSN: 1432-2218
    Keywords: Pain ; Laparoscopic cholecystectomy ; Predictors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract It is postulated that laparoscopic cholecystectomy as “patient-friendly surgery” leads to more comfort and in particular to less pain. A prospective study on pain was performed on all patients undergoing the operation over the period of 1 year (n=382) out of a series of more than 1,000 patients who have undergone the operation in our clinic. Pain was measured by a 100-point visual analogue scale (VAS), by a five-point verbal rating scale, and by the consumption of analgesics. Pain was the most frequent symptom, both before and after the operation. The mean level of pain was 37 VAS points 5 h after the operation and declined to 16 points on the third day. In 106 patients (27.8%) the intensity of pain was higher than 50 VAS points. Analgesics were used by 282 patients (73.8%), opioids by 112 (29.3%). Pain was significantly higher in female than male patients (P〈0.05), but consumption of analgesics was similar in both groups. The most severe pain was localized to the abdominal wall wounds by 157 (41.1%) and to the right upper abdomen by 138 patients (36.1%) on the first postoperative day. Patients who needed opioids and/or had a pain level of 〉50 VAS points (n=138) had higher preoperative pain levels (P=0.018) and preoperatively complained more frequently about nausea, vomiting, bloating, and a feeling of abdominal pressure (P=0.003–0.031). However, predictive values of these variables were too small to be of clinical benefit. The duration of operation, intraoperative events (loss of bile, blood, or gallstones), and additional laparoscopic procedures (adhesiolysis, lavage, extension of an incision, suture of fascia) did not influence the intensity of postoperative pain. We conclude that laparoscopic cholecystectomy did cause significant postoperative pain in one-third of our patients only up to the first postoperative day. As predictors for high intensity of pain were not identified, pain should be monitored and analgesics should be delivered liberally.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract In the CERN NA 32 experiment a high-resolution silicon vertex detector and a purely topological approach have been used to investigate various decays of charmed particles. We observe ∼620 fully reconstructed decays ofD 0 in 12 channels and determine the branching ratios. For fourD 0 decay modes involving a single (unseen) π0 the small and narrowD *+−D 0 mass difference is used to measure their branching ratios. We also observe ∼280 fully reconstructedD + decays in 10 channels, ∼90D s + decays in 11 channels as well as 160Λ s c and 18 decay channels ofD +. For theD s + , we measure the branching fractions within a subset of 16 three- and five-prong decay channels. For theΛ s c , we determine the branching fractions within a sample of 11 three- and five-prong decay channels, nearly all involving a proton.
    Type of Medium: Electronic Resource
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