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  • 1985-1989  (1)
  • 1980-1984  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Detection of complement activation in human serum using C5S-Induced chemiluminescence of human granulocytes (1985)
    Springer
    Archives of dermatological research 278 (1985), S. 41-43 
    Details
    ISSN: 1432-069X
    Keywords: Complement ; Anaphylatoxins ; C5a ; Chemiluminescence ; Granulocytes ; Lucigenin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The complement system can be activated by many factors, including immune complexes, leading to the generation of biologically active split products like C5a anaphylatoxin. This study presents a technique which may be used for measuring C5a activity in human serum. The tetanus-anti-tetanus immune complex and aggregated human IgG were used as model activators of the complement cascade. The C5a activity was measured by C5a-induced chemiluminescence of granulocytes; furthermore, a radioimmunoassay was used to detect the C5a peptide. There was a strongly positive correlation between the two assay systems. The described method should be useful as an alternative means of detecting complement activation in the serum of patients with inflammatory diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412494
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Differential generation of chemiluminescence — detectable oxygen radicals by normal polymorphonuclear leukocytes challenged with sera from systemic lupus erythematosus and rheumatoid arthritis patients (1984)
    Springer
    Journal of molecular medicine 62 (1984), S. 710-716 
    Details
    ISSN: 1432-1440
    Keywords: Polymorphonuclear leukocytes ; Chemiluminescence ; Immune complexes ; Systemic lupus erythematosus ; Rheumatoid arthritis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The generation of chemiluminescence (CL)-detectable oxygen radicals by normal human polymorphonuclear leukocytes (PMN) after challenging with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) sera is described. CL was measured in a luminol-dependent assay and referred to a standard obtained when preformed immune complexes (Ic) (human tetanus toxoid-antitoxoid Ic resuspended in normal pooled serum) were tested on PMN. Normal sera gave rise to CL activity by PMN between 0% and 50% of the standard Ic (mean±standard error of the mean (SEM): 20.7±4.8). Sera from SLE and RA patients induced strikingly different biological effects on PMN. SLE sera generally induced a high CL-detectable generation of oxygen metabolites which may be causally related to the intense tissue damage (vasculitis) frequently observed in this disease. In contrast to SLE, RA sera induced a CL-detectable respiratory burst by PMN that was included in the normal range. Thus, the biological effects of these sera in terms of stimulation of toxic oxygen radical generation by phagocytes are quite different. This generation of oxygen radicals might reflect a different clearance of circulating Ic by PMN in SLE and RA disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01725703
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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