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  • 1985-1989  (3)
  • 1975-1979
  • 79.70  (2)
  • DIDS  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Intracellular pH regulation in the mouse lacrimal gland acinar cells (1988)
    Springer
    The journal of membrane biology 101 (1988), S. 73-81 
    Details
    ISSN: 1432-1424
    Keywords: intracellualr pH ; lacrimal gland ; amiloride ; DIDS ; Na+/H+ antiport ; Cl−/HCO 3 − ; antiport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Intracellular pH (pH i ) of the acinar cells of the isolated, superfused mouse lacrimal gland has been measured using pH-sensitive microelectrodes. Under nonstimulated condition pH i was 7.25, which was about 0.5 unit higher than the equilibrium pH. Alterations of the external pH by ±0.4 unit shifted pH i only by ±0.08 unit. The intracellular buffering value determined by applications of 25mm NH 4 + and bicarbonate buffer solution gassed with 5% CO2/95% O2 was 26 and 46mm/pH, respectively Stimulation with 1 μm acetylcholine (ACh) caused a transient, small decrease and then a sustained increase in pH i . In the presence of amiloride (0.1mm) or the absence of Na+, application of ACh caused a significant decrease in pH i and removal of amiloride or replacement with Na+-containing saline, respectively, rapidly increased the pH i . Pretreatment with DIDS (0.2mm) did not change the pH i of the nonstimulated conditions; however, it significantly enhanced the increase in pH i induced by ACh. The present results showed that (i) there is an active acid extrusion mechanism that is stimulated by ACh; (ii) stimulation with ACh enhances the rate of acid production in the acinar cells; and (iii) the acid extrusion mechanism is inhibited by amiloride addition to and Na+ removal from the bath solution. We suggest that both Na+/H+ and HCO 3 − /Cl− exchange transport mechanisms are taking roles in the intracellular pH regulation in the lacrimal gland acinar cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01872822
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cluster cations ejected from liquid metal ion source: alkali metals (Li, Na) and group IV elements (Si, Ge, Sn, Pb) (1989)
    Springer
    The European physical journal 12 (1989), S. 225-227 
    Details
    ISSN: 1434-6079
    Keywords: 36.40 ; 79.70 ; 35.20.X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Cluster abundance of Li n + (n≤19), Na n + (n≤25), Si n z+ (n≤8 forz=1, 3≤n≤7 forz=2), Ge n z+ (n≤11 forz=1, 3≤n≤9 forz=2,n=4 forz=3), Sn n z+ (n≤7 forz=1, 3≤n≤9 forz=2,n=4 forz=3) and Pb n z+ (n≤6 forz=1, 5≤n≤7 forz=2) ejected from a liquid metal ion source has been investigated by mass spectrometry. The abundance spectra of alkali metal clusters showed distinct maxima and steps atn=3, 7, 9, 13 and 19 for Li, and atn=3, 5, 11, 13 and 19 for Na. Mass spectra of Si, Ge and Sn clusters were very similar each other, showing intensity drops aftern=4 and 6 (and alson=10 for Ge) for singly charged clusters. The magic numbers observed are discussed in terms of stability of charged clusters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01426943
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Abundance of Na cluster ions ejected from a liquid metal ion source (1989)
    Springer
    The European physical journal 11 (1989), S. 87-91 
    Details
    ISSN: 1434-6079
    Keywords: 36.40 ; 79.70 ; 35.20.X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Sodium cluster ions Na+ n withn ranging up to 25 have been observed from a liquid sodium ion source by using a magnetic mass analyzer. Ion intensity as a function of cluster size showed distinct steps and local maxima atn=3, 5, 11, 13 and 19 (magic numbers), and a pronounced odd-even alternation. The features in the ion abundance curve are attributed to the relative stability of cluster ions. The observed magic numbers are only partially explained by the electronic shell model, indicating need to include a consideration of atomic structure in a cluster.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01436588
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    Paper (German National Licenses)
    Fulltext
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