ISSN:
1600-0765
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
It is now generally accepted that chronic periodontitis probably follows the accumulation of bacterial plaque on teeth surfaces. Some investigators have claimed to show that substances from plaque damage cells in vitro, but their results appear difficult to assess. The present investigation was a further attempt at examining the effect of substances in an aqueous plaque extract on mammalian cell cultures in vitro. In addition, this effect was compared with that caused by substances in saliva. Plaque was collected onto ice from patients requiring dental treatment. The plaque was pooled, and homogenised in carrier saline (Earle's saline from which NaH2PO4, NaHCO3, and glucose had been omitted). The mixture was homogenised at 4°C. centrifuged, and the supernatant fraction, the plaque extract (PE), sterilised by millipore filtration. Unstimulated whole saliva was centrifuged, and the supernatant fraction sterilised in the same way as the Pe # HeLa, L 929, and BHK 21 C 13 established cell cultures, and foetal human lung fibroblasts at 4th passage in cell culture, were grown in Eagle's medium with Earle's saline, calf serum to 10% v/v, and penicillin and streptomycin each to 100 units per ml. The PE was found to contain material which damaged HeLa cells, and prevented the growth of all 4 cell types. This material did not seem to detach any of these cells from the dish, and was 20 times more concentrated in plaque than in saliva. These results indicate that mammalian cell cultures can be used to detect toxic substances in aqueous plaque extracts. These substances may possibly irritate junctional epithelium at the base of the gingival sulcus or pocket in vivo.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1600-0765.1973.tb01120.x
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