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  • 1985-1989  (592)
  • Engineering  (304)
  • Immunohistochemistry  (120)
  • Immunocytochemistry  (113)
  • Magnetic resonance imaging
  • Nuclear reactions
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  • 1
    Digitale Medien
    Digitale Medien
    Differential immunocytochemical staining for glial fibrillary acidic (GFA) protein, S-100 protein and glutamine synthetase in the rat subcommissural organ, nonspecialized ventricular ependyma and adjacent neuropil (1986)
    Springer
    Cell & tissue research 245 (1986), S. 343-351 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Subcommissural organ ; Ependyma ; Astrocytes ; Immunocytochemistry ; Glial fibrillary acidic (GFA) protein ; S-100 protein ; Glutamine synthetase ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Antibodies raised against glial fibrillary acidic protein (GFA), S-100 protein (S100) and glutamine synthetase (GS) are currently used as glial markers. The distribution of GFA, S100 and GS in the ependyma of the rat subcommissural organ (SCO), as well as in the adjacent nonspecialized ventricular ependyma and neuropil of the periaqueductal grey matter, was studied by use of the immunocytochemical peroxidase-antiperoxidase technique. In the neuropil, GFA, S100 and GS were found in glial elements, i.e., in fibrous (GFA, S100) and protoplasmic astrocytes (S100, GS). The presence of S100 in the majority of the ventricular ependymal cells and tanycytes, and the presence of GFA in a limited number of ventricular ependymal cells and tanycytes confirm the glial nature of these cells. The absence of S100, GFA and GS from the ependymocytes of the SCO, which are considered to be modified ependymal cells, suggests either a non-astrocytic lineage of these cells or an extreme specialization of the SCO-cells as glycoprotein-synthesizing and secreting elements, a process that may have led to the disappearance of the glial markers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213941
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  • 2
    Digitale Medien
    Digitale Medien
    Magnetic resonance imaging of cor triatriatum (1988)
    Springer
    Pediatric cardiology 9 (1988), S. 149-151 
    Details
    ISSN: 1432-1971
    Schlagwort(e): Cor triatriatum ; Magnetic resonance imaging ; Phase display
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Magnetic resonance imaging utilizing spin echo sequences was used to demonstrate cor triatriatum in an 18-year-old boy. Phase map images aided the diagnosis by demonstrating the presence of slowly flowing blood in the accessory atrial chamber. Magnetic resonance is an excellent modality for the noninvasive diagnosis of cor triatriatum.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02080555
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  • 3
    Digitale Medien
    Digitale Medien
    Comparative marker analysis of the ependymocytes of the subcommissural organ in four different mammalian species (1989)
    Springer
    Cell & tissue research 257 (1989), S. 255-262 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Subcommissural organ ; Glial markers ; Immunocytochemistry ; GABA uptake ; Comparative analysis ; Mammals (rat, cat, mouse, rabbit)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The subcommissural organ (SCO), classified as one of the circumventricular organs, is composed mainly of modified ependymal cells, attributable to a glial lineage. Nevertheless, in the rat, these cells do not possess glial markers such as glial fibrillary acidic protein (GFAP), protein S100, or the enzyme glutamine synthetase (GS). They receive a synaptic 5-HT input and show pharmacological properties for uptake of GABA resembling the uptake mechanism of neurons. In this study, we examine the phenotype of several mammalian SCO (cat, mouse, rabbit) and compare them with the corresponding features of the rat SCO. In all these species, the SCO ependymocytes possess vimentin as an intermediate filament, but never express GFAP or neurofilament proteins. They do not contain GS as do glial cells involved in GABA metabolism, and when they contain protein S100 (rabbit, mouse), its rate is low in comparison to classical glial or ependymal cells. Thus, these ependymocytes display characteristics that differentiate them from other types of glial cells (astrocytes, epithelial ependymocytes and tanycytes). Striking interspecies differences in the capacity of SCO-ependymocytes for uptake of GABA might be related to their innervation and suggest a species-dependent plasticity in their function.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00261828
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  • 4
    Digitale Medien
    Digitale Medien
    Magnetic resonance imaging of avascular necrosis of the femoral head (1986)
    Springer
    Skeletal radiology 15 (1986), S. 133-140 
    Details
    ISSN: 1432-2161
    Schlagwort(e): Magnetic resonance imaging ; Osteonecrosis ; Bone disease, hip
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract This study investigates the role of magnetic resonance imaging (MR) in identifying avascular necrosis (AVN) of the femoral head and in monitoring its therapy. The detection of AVN, particularly in its early stages, is imperative to give therapeutic intervention the best opportunity for successful management. The results of magnetic resonance imaging are compared with those of the standard diagnostic modalities in evaluation of patients with the lesion. Examinations were performed at 0.12 T with a repetition time (TR) of 143 ms and times to echo (TE's) of 10 or 20 ms. This study represents a retrospective review of 90 hips which were examined in 45 consecutive patients. Of these, 52 hips were biopsied as part of treatment. MR was shown to be sensitive in the detection of AVN. Comparison of MR with radionuclide imaging showed comparable sensitivity and specificity. MR was also noted to be sensitive in the detection of early AVN. Preliminary results suggest that MR can monitor treatment of the affected hip, and may even be able to predict patient response to therapy. Although further work is necessary to determine the role of MR in the evaluation of the patient presenting with hip pain, MR is a sensitive method in detecting AVN and in monitoring its course in patients suspected of having the disease.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00350207
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  • 5
    Digitale Medien
    Digitale Medien
    Dendritic reticulum cells in AIDS-related lymphadenopathy (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1173-1175 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Immunohistochemistry ; immunologic deficiency syndromes ; lymph nodes ; protease inhibitors
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary One of two cases of acquired immune deficiency syndrome-related persistent generalized lymphadenopathy revealed a profoundly altered pattern of dendritic reticulum cells as demonstrated by immunoreactive acid cysteine proteinase inhibitor. The alterations could be related to totally or partially destructed lymphoid secondary follicles.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01951713
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  • 6
    Digitale Medien
    Digitale Medien
    Small cell neuroendocrine carcinoma of the urinary bladder (1988)
    Springer
    Virchows Archiv 413 (1988), S. 505-512 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Neuroendocrine differentiation ; Immunohistochemistry ; Electron microscopy ; Small cell undifferentiated bladder carcinoma
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Small cell carcinoma with the histological appearance of pulmonary small cell carcinoma is a rare tumour in the urinary bladder. In previous case reports the neuroendocrine nature of small cell bladder carcinoma has been accepted, but on review the evidence for true neuroendocrine differentiation appears unsatisfactory. In this study the histological, immunohistochemical and ultrastructural characteristics of three cases of small cell carcinoma of the urinary bladder are described. Ultrastructurally, the cytoplasm of all three tumours contained neurosecretory-type granules and each of the tumours demonstrated positive immunoreaction for two or more neuroendocrine markers, from a panel including neuron-specific enolase, chromogranin A, Leu-7, bombesin and synaptophysin. Although the combination of ultrastructural and immunohistochemical examination obviously offers the strongest evidence in establishing neuroendocrine differentiation, it is argued that immunohistochemistry alone may also yield important information in demonstrating a neuroendocrine nature, provided that at least neuron-specific enolase and synaptophysin are included as markers. The clinical relevance of identifying neuroendocrine differentiation in small cell bladder carcinoma is suggested by the favourable response to combination chemotherapy in two of our cases.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00750391
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  • 7
    Digitale Medien
    Digitale Medien
    Primary duodenal carcinoma arising in a non-vaterian tubulo-villous adenoma (1989)
    Springer
    Virchows Archiv 414 (1989), S. 529-533 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Duodenal tubulo-villous adenoma ; Adenocarcinoma ; Immunohistochemistry ; Diameter of adenoma
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Primary duodenal carcinoma and duodenal adenoma are rare tumours. Duodenal carcinoma makes up about 0.3% of all malignant tumours of the gastrointestinal tract (Alwmark et al. 1980; Spira et al. 1977). The present paper describes a duodenal carcinoma arising in a mixed tubulo-villous non-Vaterian adenoma in a 68 year old male. Immunocytochemical analysis revealed evidence of neuroendocrine differentiation in both adenoma and carcinoma. In a review of the literature a correlation between the size of adenoma and the probability of concomitant carcinoma is demonstrated. Duodenal adenoma measuring more than 4 cm in diameter should be considered potentially malignant.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00781711
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  • 8
    Digitale Medien
    Digitale Medien
    The conducting tissue in the adult chicken atria (1985)
    Springer
    Anatomy and embryology 172 (1985), S. 239-245 
    Details
    ISSN: 1432-0568
    Schlagwort(e): Conducting tissue ; Chicken atria ; Immunohistochemistry ; Myosins
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary 1. A three-dimensional reconstruction from serial sections of adult chicken heart was made to verify whether Purkinje cells, that can be recognized by a number of wellknown histological criteria, form specialized tracts in the adult chicken atria. This reconstruction revealed a loosely arranged network of Purkinje cells connecting the two atria. This network has not been described before. No tracts could be detected between the sinoatrial and the atrioventricular nodes. 2. These atrial Purkinje cells express the atrial and ventricular myosin isoform, as determined by the use of monoclonal antibodies that were prepared against atrial and ventricular myosin isoform, respetively. 3. Some atrial myocytes that are topographically closely related to the Purkinje cells and that cannot be distinguished from the surrounding myocytes with conventional histological criteria, express, apart from the atrial myosin isoform, also the ventricular myosin isoform. 4. The similar expression pattern of these two cell types and their close topographical relationship suggest the presence of a more elaborate system specialized in conduction than the well-known conductive system found with conventional histological techniques.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00319606
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  • 9
    Digitale Medien
    Digitale Medien
    Similarities between aberrant serotonergic fibers in the aged and 5,7-DHT denervated young adult rat brain (1989)
    Springer
    Experimental brain research 78 (1989), S. 81-89 
    Details
    ISSN: 1432-1106
    Schlagwort(e): Aging ; Serotonin ; Degeneration ; 5,7-DHT ; Lesion ; Immunohistochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Recent morphological observations have suggested neurotransmitter specific degeneration of amongst others, the serotonergic system in the aged rat brain. However, morphological studies can only give a static picture of the events that take place over a period of several months. In the present study we used an experimental model in which degeneration of the serotonergic system in the young adult rat brain was produced on a short time scale. Morphological changes were studied 2 h and 1 or 14 days after intracerebroventricular injection of 5,7-dihydroxytryptamine (5,7-DHT). Nonspecific damage and severe depletion of serotonergic fibers was observed in the immediate surroundings of the injection site, representing the effects of high local concentrations of 5,7-DHT. Sometime after injection swollen varicosities and dilated non-varicose fibers were observed. Fourteen days after the 5,7-DHT treatment cluster-like fibers appeared. It is argued that these swollen and crumpled fiber knots are slowly degenerating fibers. A comparison is made with the abnormal serotonergic fibers in the aged rat brain and it is concluded that these aged abnormal fibers represent axonal degeneration of the serotonergic system in the senescent rat brain.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00230689
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  • 10
    Digitale Medien
    Digitale Medien
    Magnetic resonance imaging and angiography of the brain in embolic left atrial myxoma (1989)
    Springer
    Neuroradiology 31 (1989), S. 137-139 
    Details
    ISSN: 1432-1920
    Schlagwort(e): Magnetic resonance imaging ; Cerebral angiography ; Atrial myxoma
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary A case of left atrial myxoma presenting exclusively with neurological symptoms, studied with magnetic resonance imaging (MRI) combined with cerebral angiography and computed tomography (CT) is reported. Typical angiographic findings suggested the diagnosis of myxoma. MRI showed multiple ischemic lesions disseminated throughout the entire brain, some of which had been clinically asymptomatic. Because of its sensitivity in identifying small cerebral infarcts, MRI should prove in the future to be a first-choice technique in the evaluation of the presence of and extent of cerebral involvement in embolic left atrial myxoma.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00698841
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