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  • 1
    Electronic Resource
    Electronic Resource
    Endogenous cytokinins in vegetative shoots of peas (1986)
    Springer
    Plant growth regulation 4 (1986), S. 311-323 
    Details
    ISSN: 1573-5087
    Keywords: Pea ; Pisum ; G2 ; senescence ; cytokinin ; zeatin ; zeatin riboside ; zeatin-0-glucoside ; zeatin riboside-0-glucoside ; dihydrozeatin ; dihydrozeatin riboside ; zeatin-7-glucoside ; zeatin-9-glucoside ; mass spectrometry ; selected ion monitoring ; stable isotope dilution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In G2 peas senescence only takes place in long days. In order to determine the role of cytokinins in this process the endogenous cytokinins from vegetative shoots of G2 peas were characterized using gas chromatography-mass spectroscopy following purification by HPLC. Cytokinins were extracted and purified with and without the addition of 15N labelled internal standards of several cytokinins to estimate cytokin content by isotope dilution in the mass spectra. Samples without internal standards were bioassayed after HPLC. Bioassays showed the presence of zeatin, zeatin riboside and zeatin-0-glucoside. The presence of zeatin was confirmed by its mass spectrum of its permethylated derivative. Tentative identification of zeatin riboside, zeatin-0-glucoside, dihydrozeatin, and dihydrozeatin-0-glucoside was obtained by the coincidence of the major ion for the permethylated natural and 15N labelled internal standards on GC-MS, and the similar coincidence of ions for permethylated zeatin riboside-0-glucoside by direct probe MS. There was no indication of the presence of significant quantities of zeatin-7-glucoside or zeatin-9-glucoside. The amounts in the tissue ranged from 200–1000 ng/kg fresh weight for each cytokinin and about 2–4 μg/kg fresh weight for total cytokinins. There was no apparent difference in the levels in mature but pre-senescent shoots grown in long days and short days indicating that apical senesecence in G2 peas does not appear to be induced by a decline in cytokinin level in the shoots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024931
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  • 2
    Electronic Resource
    Electronic Resource
    The value of mutants unable to carry out photorespiration (1988)
    Springer
    Photosynthesis research 16 (1988), S. 155-176 
    Details
    ISSN: 1573-5079
    Keywords: Hordeum ; mutants ; nitrogen metabolism ; photosynthesis ; photorespiration ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Manipulation of the CO2 concentration of the atmosphere allows the selection of photorespiratory mutants from populations of seeds treated with powerful mutagens such as sodium azide. So far, barley lines deficient in activity of phosphoglycolate phosphatase, catalase, the glycine to serine conversion, glutamine synthetase, glutamate synthase, 2-oxoglutarate uptake and serine: glyoxylate aminotransferase have been isolated. In addition one line of pea lacking glutamate synthase activity and one barley line containing reduced levels of Rubisco are available. The characteristics of these mutations are described and compared with similar mutants isolated from populations of Arabidopsis. As yet, no mutant lacking glutamine synthetase activity has been isolated from Arabidopsis and possible reasons for this difference between barley and Arabidopsis are discussed. The value of these mutant plants in the elucidation of the mechanism of photorespiration and its relationships with CO2 fixation and amino acid metabolism are highlighted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039491
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  • 3
    Electronic Resource
    Electronic Resource
    Manipulation of the polyamine content and senescence of apical buds of G2 peas (1985)
    Springer
    Plant growth regulation 3 (1985), S. 401-417 
    Details
    ISSN: 1573-5087
    Keywords: Pea ; Pisum ; apical senescence ; defruiting ; polyamines ; putrescine ; spermidine ; spermine ; polyamine ; synthesis inhibitors ; gibberellin ; 1-aminocyclopropane-1-carboxylic acid ; aminoethoxyvinyl glycine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of various treatments on the apical senescence and polyamine content of apical buds of G2 peas was analysed. Defruiting prevented senescence and increased bud size and polyamine content. Exogenous applications of GA20 enhanced bud size and spermidine concentration. Applied spermidine had a slight effect on spermidine level but did not delay senescence. ACC strongly induced adecrease in bud size and, at 10 mM, apical senescence. This was accompanied by a steady decline in the level of all polyamines though their concentration remained constant until 10 mM ACC, where a drop was noted. Spermidine in the presence of ACC modulated the effect of ACC on the bud size while returning the internal polyamine content to control levels. AVG, an inhibitor of ACC synthesis produced pronounced increases in putrescine though no apparent effect on apical bud growth. Polyamine synthesis inhibitors were without effect on growth or internal polyamine content. The internal polyamine content appeared to correlate with apical bud size and vigor but did not show any consistent relationship to apical bud senescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00117596
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  • 4
    Electronic Resource
    Electronic Resource
    Uptake and retention of3H-estradiol by gonadotrophs and lactotrophs in the pituitary glands of the guinea pig, hamster and gerbil (1987)
    Springer
    Cell & tissue research 248 (1987), S. 75-78 
    Details
    ISSN: 1432-0878
    Keywords: LH-cells ; Prolactin cells ; Immunocytochemistry ; Estrogen ; Autoradiography ; Guinea pig ; Hamster ; Gerbil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Nuclear uptake and retention of3H-estradiol by luteinizing hormone (LH) and prolactin (PRL) cells was examined in three species of rodents (guinea pigs, hamsters and gerbils) using the combined techniques of immunocyto-chemistry and autoradiography. Castrated animals were injected with3H-estradiol and decapitated 1.5 h later. The pituitary glands were processed for thaw-mount autoradiography followed by conventional immunocytochemical staining for LH and PRL.3H-estradiol accumulated in more than 80% of the anterior pituitary cells in the gerbils, while only 33 and 22% of the cells accumulated3H-estradiol in the hamsters and guinea pigs, respectively. A varying percentage of immunoreactive LH and PRL cells in all three species were found also to contain binding sites for estradiol. Some LH and PRL cells in hamsters and guinea pigs and only some in PRL cells of gerbils were found to be devoid of grains. Quantitative analysis revealed that the number of grains per nucleus differed considerably from cell to cell. LH cells of guinea pigs accumulated much larger amounts of3H-estradiol than did the PRL cells, while the LH cells in the hamsters and gerbils accumulated only slightly more3H-estradiol than the PRL cells. These results confirm the previous observations in rats and baboons that demonstrated tremendous species differences in percentage of cells in the anterior pituitary gland that accumulated3H-estradiol. Also, these data suggest that there are functionally heterogeneous cell types among the LH and PRL cells in hamsters, guinea pigs and gerbils as has been previously demonstrated in rats and baboons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01239965
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  • 5
    Electronic Resource
    Electronic Resource
    A new type of methylamine oxidase: The sole oxidase produced during growth of Sporobolomyces albo-rubescens on primary alkylamines (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 87-92 
    Details
    ISSN: 0749-503X
    Keywords: Amine oxidase ; yeast ; methylamine ; n-butylamine ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Under conditions known to separate methylamine oxidase from benzylamine oxidase in other yeast strains, only a single oxidase could be detected in Sporobolomyces albo-rubescens. This occurred irrespective of whether methylamine or n-butylamine was the nitrogen source for growth. The oxidase did not attack benzylamine. It was concluded that this organism can only produce a methylamine oxidase. The enzyme was purified to 90% homogeneity and found to have properties significantly a methylamine oxidases previously characterised. It lost only 40% of its activity in 30 min at 45°C, whereas methylamine oxidase previously described had half-lives of from 2 to 9 min at 45°C. It showed also a lower activity with short chain 1-aminoalkanes and a higher activity with longer chain 1-aminoalkanes than other methylamine oxidases, and had a significantly smaller subunit molecular weight (57 000 compared with 80 000).
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020203
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  • 6
    Electronic Resource
    Electronic Resource
    The subcellular location of 4-aminobutyrate aminotransferase in Candida boidinii and its probable role in the breakdown of putrescine and spermidine (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 4 (1988), S. 149-153 
    Details
    ISSN: 0749-503X
    Keywords: Aminotransferase ; transaminase ; 4-aminobutyrate ; Candida ; putrescine ; spermidine ; cytisol ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: 4-Aminobutyrate aminotransferase (EC 2.6.1.19) was elevated in activity by 20- to 40-fold in cells of the yeast Candida boidinii grown on spermidine, putrescine, 4-acetamidobutyrate and 4-aminobutyrate compared with activities detected in cells grown on ammonium, methylamine or 6-aminohexanoate, confirming previous suggesions that it plays a key role in polyamine breakdown. Other enzymes of the proposed route of polymine breakdown were found to be non-coordinately induced or derepressed during growth on spermidine or its putative breakdown intermediates. The enzyme was not sedimented from spheroplast lysates at 100 000 × g, and it is concluded that it is conclded that it is probably cytosoilc in its subcellular location (although the data do not exclude the possiblity of its being vacuolar).
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320040209
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  • 7
    Electronic Resource
    Electronic Resource
    Effects of high-temperature treatments on development, viability, and heat-shock response in a temperature-sensitive cell-lethal mutant of Drosophila melanogaster (1985)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 6 (1985), S. 27-37 
    Details
    ISSN: 0192-253X
    Keywords: Drosophila ; temperature effects ; heat-shock ; cell-lethal mutation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Pulses of various durations at temperatures between 29 and 38°C were applied to developing larvae of Drosophila melanogaster carrying the temperature-sensitive cell-lethal mutation 1 (1)ts726. The results show that it is not possible to reduce the time required for the induction of abnormalities in the mutant by treating larvae with heat pulses at temperatures higher than 29°C. Instead, treatment with high temperature leads to fewer abnormalities than 29°C treatments. Furthermore with high temperature treatments, the mutation has less effect on viability than is seen at 29°C. It is suggested that 1 (1)ts726 leads to abnormalities and death by a temperature-induced imbalance between different physiological or development events, rather than by interfering with the ability of the cell or the organism to withstand high temperature in general.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020060103
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  • 8
    Electronic Resource
    Electronic Resource
    G-proteins in the signal-transduction pathways of Dictyostelium discoideum (1988)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 9 (1988), S. 215-226 
    Details
    ISSN: 0192-253X
    Keywords: cAMP ; cGMP ; chemotaxis ; mutant ; desensitization ; receptor ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The functional interaction of surface cAMP receptors with effector enzymes via G-proteins was investigated in Dictyostelium discoideum. Several experimental conditions were used to investigate signal transduction, such as reduced temperatures, use of down-regulated cells and of mutants. The results are presented as a model describing the complex interaction between multiple forms of the surface cAMP receptor and different G-proteins that are responsible for the generation of the second messengers, cAMP, cGMP, InsP3 and Ca2+.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020090404
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  • 9
    Electronic Resource
    Electronic Resource
    Degradation of organic nitrogen compounds by yeasts (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 1-34 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020102
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