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  • 1985-1989  (4)
  • Calcium  (2)
  • Cell & Developmental Biology  (2)
  • Secale cereale
  • 1
    Electronic Resource
    Electronic Resource
    Abolition of the orthodromically evoked IPSP of CA1 pyramidal cells before the EPSP during washout of calcium from hippocampal slices (1987)
    Springer
    Experimental brain research 65 (1987), S. 676-680 
    Details
    ISSN: 1432-1106
    Keywords: Calcium ; IPSP ; EPSP ; Hippocampus ; Area CA1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The complex EPSP-IPSP response of CA1 pyramidal cells to orthodromic activation of Schaffer collaterals-commissural afferent fibres was monitored during washout of Ca from hippocampal slices. The IPSP was clearly abolished before the EPSP and this occurred when the extracellular Ca([Ca2+]o) had fallen to between 1.03 and 0.7 mM. The loss of the IPSP was usually associated with a temporary increase in size of the EPSP and sometimes a membrane depolarization and the appearance of spontaneous activity. As [Ca2+]o fell further these effects were reversed and eventually the EPSP was abolished when [Ca2+]o had reached between 0.78 and 0.26 mM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235992
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of Ga3 and Ca2+ on barley aleurone protoplasts: a freeze-fracture study (1988)
    Springer
    Protoplasma 146 (1988), S. 157-165 
    Details
    ISSN: 1615-6102
    Keywords: Calcium ; Endomembrane system ; Enzyme secretion ; Freeze fracture ; Gibberellic acid ; Protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Freeze-fracture electron microscopy was used to study changes in the endomembrane system of barley (Hordeum vulgare L. cv. Himalaya) aleurone protoplasts. Protoplasts were used for this study because their response to calcium and the plant hormone gibberellic acid (Ga3) can be monitored prior to rapid freezing of cells for electron microscopy. Protoplasts incubated in Ga3 plus Ca2+ secrete elevated levels of a-amylase relative to cells incubated in Ga3 or Ca2+ alone. The endoplasmic reticulum (ER) and Golgi apparatus of protoplasts incubated in Ga3 plus Ca2+ undergo changes that are well correlated with the synthesis and secretion of a-amylase. The ER, which appears as short, single sheets of membrane in Ca2+-and Ga3-treated protoplasts, exists as a series of long fenestrated stacks of membranes following incubation in Ga3 plus Ca2+. The Golgi apparatus is also more highly developed in protoplasts treated with Ga3 plus Ca2+. This organelle is larger and has more vesicles associated with its periphery in protoplasts that actively secrete a-amylase. Evidence that the Golgi apparatus participates in a-amylase secretion is also provided by experiments with the ionophore monensin, which causes pronounced swelling of Golgi cisternae and inhibits the secretion of a-amylase. We interpret these observations as showing that the ER and Golgi apparatus of barley aleurone participate in the intracellular transport and secretion of a-amylase. The plasmalemma (PF face) of barley aleurone protoplasts shows a high density of intramembranous particles (IMPs) which, in general, are evenly distributed. Occasionally, ordered arrays of IMPs are observed, possibly resulting fro m osmotic stress. after 48 hours the plasmalemma of some Ga3-treated protoplasts show particle-free areas considered to be indications of senescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01405925
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Phenytoin-induced connective tissue growth in the rat (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 215 (1986), S. 99-105 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Rats were treated daily for 9 days with 100, 50, or 25 mg/kg phenytoin i.p. This treatment resulted in a significant increase in the thickness of the connective tissue capsules of the liver, spleen, and pancreas, and of the subepithelial connective tissue of the mesentery but not the epicardium or visceral pleura of the lung where exposure to the drug was via the vascular route. Many areas of connective tissue growth exhibited obvious proliferation of fibroblasts and in some areas contained seemingly large numbers of macrophages and an increase in vascularity. It was demonstrated by electron microscopy that the macrophages occasionally were seen in intimate contact with the fibroblasts.Our observations clearly showed that intraperitoneal exposure of visceral connective tissues of the rat to phenytoin rapidly resulted in a dose-related proliferation of that tissue. The presence of numerous macrophages leads to the suggestion that macrophage-derived growth factor could be responsible for the increased growth.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092150203
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Analytical electron microscopy study of high Tc superconductor YBa2Cu3O7 (1988)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 8 (1988), S. 307-309 
    Details
    ISSN: 0741-0581
    Keywords: Electron energy loss spectroscopy ; Varying O2 content ; Crystallographic orientation dependence of oxygen near edge features ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The high Tc superconducting material YBa2Cu3O7 shows a complex relationship between microstructure and oxygen content, which are controlled by length of heat treatment, atmosphere, and quench rate. An AEM investigation studying changes in the oxygen near edge features was undertaken. Electron energy loss spectroscopy (EELS) measurements have the important advantage that they can be made on single crystal grains, allowing orentation-dependent studies. Both ion-milled and crushed samples with varying O2 content were analyzed. The structure of YBaCu3O7 was determined by neutron diffraction to be orthorhombic with distinct Cu-O chains along the b-axis as well as Cu-O planes in the a - b plane. Therefore, by looking for a crystallographic dependence of the oxygen K-edge one might be able to distinguish inequivalent oxygen atoms by their core level binding energy and correlate site occupancy with varying O2 content. The EELS results on the oxygen K-edge are strongly dependent on oxygen content, most noticeably when the c-axis is parallel to the electron beam.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060080309
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    Paper (German National Licenses)
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