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  • 1985-1989  (2)
  • ColE1 replication  (1)
  • DNA gyrase  (1)
  • Illegitimate recombination
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  • 1
    Electronic Resource
    Electronic Resource
    Formation of deletion in Escherichia coli between direct repeats located in the long inverted repeats of a cellular slime mold plasmid: Participation of DNA gyrase (1988)
    Springer
    Molecular genetics and genomics 214 (1988), S. 1-5 
    Details
    ISSN: 1617-4623
    Keywords: Escherichia coli ; Dictyostelium ; DNA gyrase ; Deletion ; Plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We constructed a recombinant plasmid containing the 2.1 kb HindIII fragment of plasmid pDG1, isolated from the cellular slime mold (Dictyostelium sp. strain GA11), and using pAG60 as cloning vector. We found that deletions of the recombinant plasmid took place frequently in Escherichia coli wild-type cells. However, the deletion was not observed when the plasmid was introduced into a strain that was an isogenic temperature-sensitive mutant of the gyrA gene. These results suggest that E. coli DNA gyrase is involved in the mechanisms of the deletion formation. It was shown that the 1.0 kb deletant derived from the 2.1 kb HindIII insert was produced by elimination of a 1.1 kb region. Sequence analysis of the deletants showed that cutting and rejoining took place between two out of the six nearly perfect direct repeats [21 bp palindromic sequences; AAAAAA(T/C)GGC(G/C)GCC(A/G)TTTTTT], located near the distal ends of the inverted repeats, preserving one copy of the repeats. These sequences consist of local short inverted repeats, where cutting and rejoining occur at one of the two regions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00340170
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and characterization of herC, a mutation of Escherichia coli affecting maintenance of ColE1 (1989)
    Springer
    Molecular genetics and genomics 219 (1989), S. 333-340 
    Details
    ISSN: 1617-4623
    Keywords: ColE1 replication ; Primer RNA ; Suppressor mutation ; RF2 ; Ribonuclease H
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two modes of ColE1 DNA replication are known, one dependent on RNase H, and the other RNase H independent. The cer114 mutant of the ColE1 replicon is defective in both modes and carries a single base pair alteration 95 by upstream of the replication origin. An Escherichia coli mutant which restored maintenance of the cer114 replicon was isolated. This host suppressor mutant is defective in RNase H and carries a herC, mutation located at 62 min of the E. coli chromosome. The herC, mutation is recessive to its wild-type allele and supports maintenance of the mutant replicon in the absence of RNase H. The herC, mutation alone conferred cold-sensitive growth, suggesting that the herC, gene product is essential for cell growth. The 1832 by E. coli DNA fragment, containing the wild-type allele of the herC, mutation, was cloned and an open reading frame for the HerC protein was determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00259604
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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