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  • 1
    Digitale Medien
    Digitale Medien
    Formation of deletion in Escherichia coli between direct repeats located in the long inverted repeats of a cellular slime mold plasmid: Participation of DNA gyrase (1988)
    Springer
    Molecular genetics and genomics 214 (1988), S. 1-5 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Escherichia coli ; Dictyostelium ; DNA gyrase ; Deletion ; Plasmid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary We constructed a recombinant plasmid containing the 2.1 kb HindIII fragment of plasmid pDG1, isolated from the cellular slime mold (Dictyostelium sp. strain GA11), and using pAG60 as cloning vector. We found that deletions of the recombinant plasmid took place frequently in Escherichia coli wild-type cells. However, the deletion was not observed when the plasmid was introduced into a strain that was an isogenic temperature-sensitive mutant of the gyrA gene. These results suggest that E. coli DNA gyrase is involved in the mechanisms of the deletion formation. It was shown that the 1.0 kb deletant derived from the 2.1 kb HindIII insert was produced by elimination of a 1.1 kb region. Sequence analysis of the deletants showed that cutting and rejoining took place between two out of the six nearly perfect direct repeats [21 bp palindromic sequences; AAAAAA(T/C)GGC(G/C)GCC(A/G)TTTTTT], located near the distal ends of the inverted repeats, preserving one copy of the repeats. These sequences consist of local short inverted repeats, where cutting and rejoining occur at one of the two regions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00340170
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  • 2
    Digitale Medien
    Digitale Medien
    Ultraviolet-induced mutation of bacteriophage λ (1974)
    Springer
    Molecular genetics and genomics 134 (1974), S. 21-27 
    Details
    ISSN: 1617-4623
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Clear mutations of bacteriophage λ are increased within F- recipients in a cros s with ultraviolet (UV) irradiated F′ donors, without a direct UV irradiation of recipients. This mutagenesis occurs when phages are also irradiated with UV. The phenomenon is called an indirect UV induction of the mutation. The indirect UV induction of the mutation is also produced by a cross with UV irradiated Hfr male cells, and does not occur when the recipient is defective in recA gene.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00332809
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  • 3
    Digitale Medien
    Digitale Medien
    Suppressors of temperature-sensitive mutations in a ribosomal protein gene, rpsL (S12), of Escherichia coli K12 (1985)
    Springer
    Molecular genetics and genomics 199 (1985), S. 381-387 
    Details
    ISSN: 1617-4623
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Temperature-sensitive (ts) mutations were isolated within a ribosomal protein gene (rpsL) of Escherichia coli K12. Mutations were mapped by complementation using various transducing phages and plasmids carrying the rpsL gene, having either a normal or a defective promoter for the rpsL operon. One of these mutations, ts118, resulted in a mutant S12 protein which behaved differently from the wild-type S12 on CM-cellulose column chromatography. Suppressors of these ts mutations were isolated and characterized; one was found to be a mutation of a nonribosomal protein gene which was closely linked to the RNAase III gene on the E. coli chromosome. This suppressor, which was recessive to its wild-type allele, was cloned into a transducing phage and mapped finely. A series of cold-sensitive mutations, affecting the assembly of ribosomes at 20°C, was isolated within the purL to nadB region of the E. coli chromosome and one group, named rbaA, mapped at the same locus as the suppressor mutation, showing close linkage to the RNAase III gene.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00330746
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  • 4
    Digitale Medien
    Digitale Medien
    Studies on radiation-sensitive mutants of E. coli (1968)
    Springer
    Molecular genetics and genomics 103 (1968), S. 1-10 
    Details
    ISSN: 1617-4623
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The bacterial recA gene participates in the induction by UV irradiation of the clear mutation of phage λ and the Lac- mutation of bacteria. The necessary function is induced by irradiation of Rec+ bacteria and acts upon DNA irradiated with UV light.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00271151
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  • 5
    Digitale Medien
    Digitale Medien
    Electrophoretic variants of blood proteins in Japanese VII. Cytoplasmic glutamate-oxaloacetate transaminase (GOT1) (1986)
    Springer
    Journal of human genetics 31 (1986), S. 1-14 
    Details
    ISSN: 1435-232X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A total of 16,835 children, of whom 11,737 are unrelated, from Hiroshima and Nagasaki were examined for erythrocyte cytoplasmic glutamate-oxaloacetate transaminase (GOT1) by starch gel electrophoresis. A variant allele namedGOT1 * 2HR1 which seems to be identical withGOT1 *2 was encountered in polymorphic frequency. Five kinds of rare variants, 3NG1, 4NG1, 5NG1, 6HR1 and 7NG1 were encountered in a total of 109 children. Except for 7NG1 for which complete family study was unable, family studies confirmed the genetic nature of these rare variants, since for all instances in which both parents could be examined, one of the parents exhibited the same variant as that of their child. Thermostability profiles of these six variants were normal. The enzyme activities of five were decreased, while the value of one was normal compared to that of GOT1 1.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01876796
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