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  • 1985-1989  (2)
  • Hydrophobic interaction  (1)
  • Reversed-phase chromatography  (1)
  • 1
    ISSN: 1612-1112
    Keywords: Size-exclusion chromatography ; Polyvinyl alcohol gel column ; Oligonucleotides ; Hydrophobic interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Separation mechanisms for single-stranded oligodeoxyribo-or oligoribonucleic acid fragments were explored on an Asahipak polyvinyl alcohol gel column (GS-320) by use of sequential isomers of such molecules. Substrates having different base numbers were found to be separated by size-exclusion chromatography while those having the same numbers with different base sequences were isolated by use of the reversed-phase mode. By using those dual modes, a limit for the separation of the samples was found to arise because one mode shifted the peaks of the substrates in the sense opposite to the shift resulting from the other mode and it was found that when substrates had less than nine bases, the solutes eluted separately.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1612-1112
    Keywords: Liquid chromatography ; Reversed-phase chromatography ; Single-stranded oligo-DNAs ; Effect of silica ; Effect of the carbon content
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A reversed-phase liquid chromatographic (RPLC) column, with which large oligodeoxynucleotides (oligo-DNAs) can be separated in well-resolved peaks, has been developed. The column contains only specially selected silica bonded with monochlorodimethyloctadecylsilane. The carbon content of the column packing is the predominant factor to achieve good results. With this column several mixtures containing different large oligo-DNAs could be separated with excellent resolution.
    Type of Medium: Electronic Resource
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