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  • 1
    Electronic Resource
    Electronic Resource
    Somaclonal variation versus chemically induced mutagenesis in tomato (Lycopersicon esculentum L.) (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 733-738 
    Details
    ISSN: 1432-2242
    Keywords: Tomato ; Somaclonal variation ; Chemical mutagenesis ; Mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparison was made of the type and frequency of mutational events found in the progeny of tomato plants regenerated after one passage in vitro with those induced by chemical mutagenesis with ethyl methane sulphonate. Several mutants were recovered in the progeny of regenerated and mutagenized plants of two cultivars of tomato. They can be grouped into the following categories: seedling lethality, male sterility, resistance to Verticillium, short stature, change in number of lateral shoots or in leaf shape. The results indicate that the two sources of variability differ in their effect, changing the spectrum and frequency of the mutants as well as, at least in some cases, their pattern of segregation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00247550
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of repeated DNA sequences on direct gene transfer in protoplasts of Nicotiana plumbaginifolia (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 113-118 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana plumbaginifolia ; Protoplasts ; Gene transfer ; Repeated DNA sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Highly repeated nuclear DNA sequences from leaves of Nicotiana plumbaginifolia were cloned in pBR322 and tested for their effect on direct gene transfer in protoplasts of the same organism. Protoplasts were prepared from suspension cultures and were incubated in the presence of the plasmid pHP23 carrying the kanamycin resistance gene APH(3′)II and in the presence of the plasmids carrying the cloned sequence. DNA uptake was induced by a polyethyleneglycol (PEG) treatment. Out of the 22 tested clones, 3 significantly stimulated the frequency of appearance of transformed colonies. DNA was extracted from some of the kanamycin-resistant calli obtained by co-transformations. Dot-blots have shown that the stimulatory effect on transformation frequency is often accompanied by a consistent increase in integrated genes sequences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00299763
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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