ISSN:
1420-908X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Guinea-pig oxyntic cell tubulin has been isolated andin vitro aggregation has been studied. The spontaneous assembly of isolated tubulin was significantly accelerated and increased bt 1 mmol/l GTP. Histamine and forskolin increased tubulin polymerization only when detergent dispersed oxyntic cells or crude membranes were added. The forskolin response occurred very rapidly with an EC50 of approximately 30 μmol/l and did not require GTP. Histamine promoted tubulin aggregation with an EC50 of about 5 μmol/l only in the presence of GTP. Ranitidine completely inhibited the effects of histamine. From these data it is suggested that, in the oxyntic cell, histamine H2-receptor activated adenylate cyclase and the corresponding increase in cAMP play a role in eliciting characteristic ultrastructural changes by initiating formation of microtubules as a first step in the cascade of events leading to an increase in the secretory surface area.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02222231
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