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  • 1980-1984  (33)
  • 1970-1974  (17)
  • 1965-1969  (22)
  • 1915-1919  (10)
  • 1910-1914  (16)
  • 1890-1899  (8)
  • General Chemistry  (71)
  • Life and Medical Sciences  (24)
  • Analytical Chemistry and Spectroscopy  (8)
  • DNA  (3)
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Material
Years
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Fluorometric determination of DNA in epidermis and cultured fibroblasts, using 4′-6-diamidino-2-phenylindole (DAPI) (1984)
    Springer
    Archives of dermatological research 276 (1984), S. 52-56 
    Details
    ISSN: 1432-069X
    Keywords: Fluorometry ; DNA ; 4′-6-Diamidino-2-phenylindole ; Fibroblasts ; Epidermis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 4′-6-diamidino-phenylindole (DAPI) which is used for the detection of mycoplasmic infections of cell cultures or cytofluorometric investigations proved to be a sensitive agent for the fluorometric assay of alkali extracted DNA in cultured cells and human epidermis. Various experiments to optimize assay conditions led to the following procedure: 1 part of freeze dried tissue (1 mg/ml) and 1 part of 0.2 mol/l NaOH (w/v) were heated at 95° for 40 min. After cooling for 1 h to room temperature, 0.1 ml alkaline extract was mixed with 2.9 ml DAPI-solution (0.2 μg/ml in McIlvaine buffer containing 1 mmol/l MgCl2). After 10 min in the dark the emitted fluorescence was estimated at 453 nm with an excitation at 360 nm. This assay procedure allowed a detection limit of 25 ng DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412563
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Hormonal control of tobacco protoplast nucleic acid metabolism during in vitro culture (1981)
    Springer
    Planta 152 (1981), S. 1-7 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Cytokinin ; DNA ; Nicotiana RNA ; Protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tobacco mesophyll protoplasts cultivated in vitro do not synthesize a measurable quantity of chloroplastic ribosomal RNA, but actively synthesize cytoplasmic ribosomal RNA, polyadenylated RNA, and proteins. These syntheses are essentially independent of the presence of hormones in the culture medium and are thus related to the ageing phenomenon induced by isolation from the plant and in-vitro culture. At all stages of culture and in all culture media, protoplasts incorporate low levels of thymidine into their DNA. However, the incorporation of considerable quantities of thymidine, indicative of the S phase, only takes place after 25–30 h and requires the presence of auxin and cytokinin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384976
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Practical breast carcinoma cell kinetics: Review and update (1984)
    Springer
    Breast cancer research and treatment 4 (1984), S. 79-88 
    Details
    ISSN: 1573-7217
    Keywords: breast ; carcinoma ; DNA ; kinetics ; ploidy ; receptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The S-phase fraction (SPF) measured by flow cytometry of DNA and the thymidine labeling index (TLI) measured autoradiographically indicate the proportion of carcinoma cells currently synthesizing DNA and reflect the rate of proliferation. The TLI and SPF are lognormally distributed. The median TLI performed to maximize precursor uptake is near 5% (5 labeled carcinoma cells per 100), the mean near 7%, and the range from less than 1% to near 40%. Corresponding values for the SPF measured by DNA flow cytometry are slightly higher when appropriate measures are taken to reduce background debris counts and other artefacts. Residual elevation of SPF above TLI may result from S-phase arrested cells. Flow cytometric histograms show that clearly aneuploid cell lines exist in 50–80% of primary breast carcinomas. Aneuploid breast carcinomas have higher mean TLI than diploid breast carcinomas, and therefore proliferate more rapidly. They also more frequently lack estrogen receptor (ER). Carcinomas with minimal nuclear anaplasia, particularly those of tubular, mucinous, infiltrating lobular and adenocystic types have low TLI and SPF, whereas carcinomas with highly anaplastic nuclei, including medullary carcinomas, have high TLI and SPF. TLI and SPF correlate inversely with ER and PgR content, have no relationship to axillary lymph nodal status, and have a weak positive correlation with tumor size and a weak negative correlation with age. High TLI predicts a high risk of early relapse after primary therapy for both node-negative and node-positive carcinomas. Carcinomas that produce brain metastases have particularly high TLI. Current evidence suggests that high SPF and aneuploidy may prove to have prognostic significance like TLI.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01806389
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The distribution of monoamine oxidase and biogenic monoamines in the central nervous system of spiders (Arachnida: Araneida) (1980)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 164 (1980), S. 69-81 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The distribution and activity patterns of monoamine oxidase and monoaminergic (formaldehyde-induced) fluorescence in the central nervous system of web-building and hunting spiders have been studied using histochemical methods. Enzyme activity occurred in the neuronal perikarya and in varying intensity in the structures of the neuropile mass, but only when dopamine, adrenaline, and noradrenaline were used as substrates. The optic centres of the spider brain normally exhibited relatively strong enzyme reactions when compared with the staining intensity of the rest of the nervous system.The neuronal cell bodies contained numerous granules of yellow-green fluorescence. Monoaminergic fluorescence of the neuropile was generally a weak green. The optic mases of the hunting spiders, the anterior bridge, several commissures of the ventral cord, and the neural lamellae showed a slightly higher fluorescence intensity and single fluorescing granules.The results obtained indicate the presence of catecholamines in the spider nervous system.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051640106
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Observations on the morphology and histochemistry of the foregut muscles of spiders (Arachnida: Araneida) (1981)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 170 (1981), S. 113-131 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The morphology of the foregut muscles of five spider families (Theraphosidae, Agelenidae, Araneidae, Lycosidae, Salticidae) was described, and the individual fibre numbers and fibre cross diameters of the muscles were determined. The nomenclature of these muscles was reviewed and modified if necessary.Oxidative enzyme and myosin-ATPase histochemistry revealed eight dilatatory muscles of the foregut to consist of slow (type I) fibres, while fast fibres (type IIB), and intermediate fibres, were only to be found in the two other muscles of the foregut, and in the remaining prosomal muscles (type IIA fibres around the poison gland).The eight sucking muscles proper of the foregut also showed stronger activities of transmitter metabolizing enzymes [monoamine oxidase, glutamate dehydrogenase(NAD)], and comparatively distinct amounts of glycogen and lipids.
    Additional Material: 25 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051700108
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    On the nature, occurrence, and identity of the plasma cells of Hofbauer (1919)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 32 (1919), S. 327-349 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1050320203
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Structure of the shell from eggs of the tuatara, Sphenodon punctatus (1982)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 174 (1982), S. 197-205 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Shells from eggs of the tuatara (Sphenodon punctatus) are 0.2 mm thick and are composed of a layer of calcite and a multi-layered, fibrous shell membrane. Most of the calcareous layer is composed of roughly circular columns of crystalline material that extend deep into the shell membrane. The crystalline matrix of the columns is interwoven with fibers of the shell membrane except near the outer surface of the eggshell, where the calcareous material is more compact. Overlying the columns is a granular layer composed of blocks of crystalline material of random size, shape, and orientation. Disruption of this granular layer, perhaps through swelling of the eggs or as a result of environmental factors, gives the outer surface of the eggshell a coarse, weathered appearance. Removal of the calcareous material with a decalcifying agent shows that the outer surface of the shell membrane is composed of a meshwork of small fibers bound together by an amorphous matrix. No matrix was observed in inner layers of the shell membrane, and the fibers of these inner layers are arranged somewhat more regularly than the outer fibers. No structure comparable to the central cores of avian and certain chelonian eggs was observed in eggshells of the tuatara.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051740208
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Analysis of C1-C3 alcohols in blood by high resolution headspace gas chromatography (1982)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 5 (1982), S. 377-378 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Glass capillary columns ; Headspace analysis ; Alcohols in blood ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240050709
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  • 9
    Electronic Resource
    Electronic Resource
    Rapid and simple direct determination of porphyrins in urine by ion-pair reversed-phase high performance liquid chromatography (1980)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 85-86 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; Ion-pair reversed-phase ; Free porphyrin carboxylic acids in urine, complete separation ; Quantitation within 4.4-5.4% precision ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240030206
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  • 10
    Electronic Resource
    Electronic Resource
    Interface for capillary columns on an automatic headspace gas chromatographic system (1980)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 357-358 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary column, glass ; Interface, home-made ; Headspace analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240030711
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