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  • 1980-1984  (27)
  • 1965-1969  (21)
  • 1915-1919  (1)
  • 1910-1914  (12)
  • General Chemistry  (50)
  • Analytical Chemistry and Spectroscopy  (6)
  • DNA  (3)
  • Bone  (2)
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Material
Years
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    A new semiautomatic method for quantitative static and dynamic bone histology (1982)
    Springer
    Calcified tissue international 34 (1982), S. 439-448 
    Details
    ISSN: 1432-0827
    Keywords: Bone ; Histomorphometry ; Osteocytes ; Bone dynamics ; Histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary A new semiautomatic technique combining advantages of the manual and fully automatic methods is described for obtaining quantitative static and dynamic histologic data of bone. The hardware consists of a photomicroscope, digitizing platen, digitizer, plotter/printer, floppy disc drive, and computer. The microscope is equipped with a drawing tube through which the image of the digitizing platen is projected over the optical field. The investigator selects and traces all histologic structures to be measured by moving a cursor on the digitizing platen which is visible by its projection over the histologic field. The results on accuracy and static and dynamic precision of this method show that static and dynamic parameters of bone are obtained with a degree of error (〈20%) well within the acceptable range for biologic measurements. Comparison of this method with the grid technique according to Merz and Schenck showed that for almost all micromorphometric parameters comparable absolute data are obtained. Due to the higher precision of our method, however, the number of optical fields evaluated in obtaining these comparable data could be reduced to 25% of the number of fields evaluated by the Merz and Schenck technique. The time requirements for quantitative evaluation of a histologic slide of bone by our technique are 40–50 min; 20–25 min is needed for quantitative evaluation of osteocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02411282
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  • 2
    Electronic Resource
    Electronic Resource
    Quantitative bone histology in 84 normal American subjects (1982)
    Springer
    Calcified tissue international 34 (1982), S. 449-455 
    Details
    ISSN: 1432-0827
    Keywords: Bone ; Histomorphometry ; Normal values ; Bone biopsies ; Sampling error
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Quantitative bone histology was done in undecalcified sections of iliac crest bone specimens obtained from 84 normal American individuals. Samples were obtained within 12 h after death in a vertical and horizontal manner from both the right and left iliac crests. In addition to the determination of normal values of micromorphometric parameters of bone in these healthy American subjects, the following studies were carried out: (a) comparison of variance of micromorphometric parameters of bone obtained from the right versus left iliac bone (40 pairs), (b) comparison of micromorphometric parameters of bone obtained in a vertical versus horizontal manner (12 pairs), (c) evaluation of variance with increasing distance from the compact zone in bone samples obtained in a vertical manner (44 pairs), (d) analysis of variation between bone samples obtained more anteriorly versus posteriorly along the iliac crest (N=40), (e) comparison of differences in micromorphometric parameters obtained from age-matched men versus premenopausal women (N=12), and (f) plotting of histograms for assessment of distribution of micromorphometric parameters. The results show that histomorphometric data of bone cannot be easily compared when different techniques are employed for obtaining bone samples. Sampling variations are kept smaller when bone specimens are obtained in a vertical manner. Anterior/posterior variation does not cause major sampling error. If ranges of variation are taken into account, quantitative bone histology is a valuable tool for assessment of bone structure and bone cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02411283
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Fluorometric determination of DNA in epidermis and cultured fibroblasts, using 4′-6-diamidino-2-phenylindole (DAPI) (1984)
    Springer
    Archives of dermatological research 276 (1984), S. 52-56 
    Details
    ISSN: 1432-069X
    Keywords: Fluorometry ; DNA ; 4′-6-Diamidino-2-phenylindole ; Fibroblasts ; Epidermis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 4′-6-diamidino-phenylindole (DAPI) which is used for the detection of mycoplasmic infections of cell cultures or cytofluorometric investigations proved to be a sensitive agent for the fluorometric assay of alkali extracted DNA in cultured cells and human epidermis. Various experiments to optimize assay conditions led to the following procedure: 1 part of freeze dried tissue (1 mg/ml) and 1 part of 0.2 mol/l NaOH (w/v) were heated at 95° for 40 min. After cooling for 1 h to room temperature, 0.1 ml alkaline extract was mixed with 2.9 ml DAPI-solution (0.2 μg/ml in McIlvaine buffer containing 1 mmol/l MgCl2). After 10 min in the dark the emitted fluorescence was estimated at 453 nm with an excitation at 360 nm. This assay procedure allowed a detection limit of 25 ng DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412563
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  • 4
    Electronic Resource
    Electronic Resource
    Hormonal control of tobacco protoplast nucleic acid metabolism during in vitro culture (1981)
    Springer
    Planta 152 (1981), S. 1-7 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Cytokinin ; DNA ; Nicotiana RNA ; Protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tobacco mesophyll protoplasts cultivated in vitro do not synthesize a measurable quantity of chloroplastic ribosomal RNA, but actively synthesize cytoplasmic ribosomal RNA, polyadenylated RNA, and proteins. These syntheses are essentially independent of the presence of hormones in the culture medium and are thus related to the ageing phenomenon induced by isolation from the plant and in-vitro culture. At all stages of culture and in all culture media, protoplasts incorporate low levels of thymidine into their DNA. However, the incorporation of considerable quantities of thymidine, indicative of the S phase, only takes place after 25–30 h and requires the presence of auxin and cytokinin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384976
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  • 5
    Electronic Resource
    Electronic Resource
    Practical breast carcinoma cell kinetics: Review and update (1984)
    Springer
    Breast cancer research and treatment 4 (1984), S. 79-88 
    Details
    ISSN: 1573-7217
    Keywords: breast ; carcinoma ; DNA ; kinetics ; ploidy ; receptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The S-phase fraction (SPF) measured by flow cytometry of DNA and the thymidine labeling index (TLI) measured autoradiographically indicate the proportion of carcinoma cells currently synthesizing DNA and reflect the rate of proliferation. The TLI and SPF are lognormally distributed. The median TLI performed to maximize precursor uptake is near 5% (5 labeled carcinoma cells per 100), the mean near 7%, and the range from less than 1% to near 40%. Corresponding values for the SPF measured by DNA flow cytometry are slightly higher when appropriate measures are taken to reduce background debris counts and other artefacts. Residual elevation of SPF above TLI may result from S-phase arrested cells. Flow cytometric histograms show that clearly aneuploid cell lines exist in 50–80% of primary breast carcinomas. Aneuploid breast carcinomas have higher mean TLI than diploid breast carcinomas, and therefore proliferate more rapidly. They also more frequently lack estrogen receptor (ER). Carcinomas with minimal nuclear anaplasia, particularly those of tubular, mucinous, infiltrating lobular and adenocystic types have low TLI and SPF, whereas carcinomas with highly anaplastic nuclei, including medullary carcinomas, have high TLI and SPF. TLI and SPF correlate inversely with ER and PgR content, have no relationship to axillary lymph nodal status, and have a weak positive correlation with tumor size and a weak negative correlation with age. High TLI predicts a high risk of early relapse after primary therapy for both node-negative and node-positive carcinomas. Carcinomas that produce brain metastases have particularly high TLI. Current evidence suggests that high SPF and aneuploidy may prove to have prognostic significance like TLI.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01806389
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  • 6
    Electronic Resource
    Electronic Resource
    Analysis of C1-C3 alcohols in blood by high resolution headspace gas chromatography (1982)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 5 (1982), S. 377-378 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Glass capillary columns ; Headspace analysis ; Alcohols in blood ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240050709
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  • 7
    Electronic Resource
    Electronic Resource
    Rapid and simple direct determination of porphyrins in urine by ion-pair reversed-phase high performance liquid chromatography (1980)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 85-86 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; Ion-pair reversed-phase ; Free porphyrin carboxylic acids in urine, complete separation ; Quantitation within 4.4-5.4% precision ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240030206
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Interface for capillary columns on an automatic headspace gas chromatographic system (1980)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 357-358 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary column, glass ; Interface, home-made ; Headspace analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240030711
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Fused silica capillary columns-restoration of chromatographic efficiency for long chain alcohols (1982)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 5 (1982), S. 380-381 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, fused silica ; Thermal aging, and regeneration of fused silica capillaries ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240050711
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  • 10
    Electronic Resource
    Electronic Resource
    Zur Mechanisierung der Dokumentation in der ChemieVeröffentlicht zum 100-jährigen Bestehen der Badischen Anilin- & Sodafabrik AG., am 6. April 1965. (1965)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 77 (1965), S. 340-345 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Der Grund dafür, daß sich mechanisierte Dokumentationsverfahren in der Chemie noch nicht in großem Umfange durchsetzen konnten, liegt vorwiegend in der geringen Flexibilität der verwendeten Systeme und in der Schwierigkeit, alle Fragestellungen zu präzisieren. Für die Dokumentation von chemischen Strukturformeln, Reaktionstypen und sonstigen Sachverhalten werden Methoden beschrieben, die anpassungsfähiger als die bisher gebräuchlichen sind. Um nicht auch für einfachere Anfragen elektronische Rechenanlagen einsetzen zu müssen, wird vorgeschlagen, von einem zentralen Speicher aus kleinere Karteien mit fertigem Material zu beliefern, das maschinell nach dem jeweils bestgeeigneten Code verschlüsselt ist.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19650770711
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