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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Industrial and engineering chemistry 3 (1964), S. 381-386 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 373 (1981), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 128 (1981), S. 288-293 
    ISSN: 1432-072X
    Keywords: Clostridium aceticum ; Acetic acid formation ; Chemolithotrophic growth ; Hydrogen uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Spores of Clostridium aceticum (Wieringa) from 1947 have been revived, and a study for a redescription of this microorganism has been carried out. C. aceticum was Gram negative. The cells were rodshaped and peritrichously flagellated. Round spores were formed in terminal position. The DNA contained 33 mol % guanine plus cytosine. The organism was obligately anaerobic and grew either chemolithotrophically with H2+CO2 or chemoorgranotrophically with compounds such as fructose, l-glutamate, l-malate or pyruvate. H2 and CO2 were converted to acetic acid according to the following equation: $$2{\text{ CO}}_{\text{2}} + 4{\text{H}}_{\text{2}} \to {\text{CH}}_{\text{3}} {\text{COOH + 2H}}_{\text{2}} {\text{O}}$$ The optimal temperature for growth was 30°C. The optimal pH for chemolithotrophic growth was 8.3. The doubling times for chemolithotrophic and chemoorganotrophic growth were 25 and 8h, respectively.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 129 (1981), S. 275-280 
    ISSN: 1432-072X
    Keywords: Acetogenium kivui ; Thermophilic acetogenic bacteria ; Hydrogen oxidation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hydrogen-oxidizing acetogenic bacteria in pure culture are presently represented by the two mesophilic species, Acetobacterium woodii and Clostridium aceticum. From Lake Kivu we have isolated a Gram negative, chemolithotrophic, thermophilic anaerobe (LKT-1) that oxidizes hydrogen and reduces carbon dioxide to acetic acid. It is a non-motile, non-sporeforming rod, about 0.7μm in width and 2–7.5μm in length, often occuring in pairs or chains. The cell wall has a banded appearance; the surface layer contains a regular array of particles with six-fold rotational symmetry. No outer membrane is present. The temperature optimum for growth is 66°C, and the pH optimum is 6.4. Organic growth substrates include glucose, mannose, fructose, pyruvate, and formate; acetate is the principal product. The doubling time for growth on hydrogen and carbon dioxide is about 2h. Vitamins are neither required nor stimulatory. Yeast extract and Trypticase enhance the final yield but do not affect the growth rate. Cysteine or sulfide are required and cannot be replaced by thioglycolate or dithiothreitol. LKT-1 was mass cultured on hydrogen and carbon dioxide in a 24.1 fermentor with a yield of 34g (wet weight) of cells. The DNA base composition as determined by buoyant density is 38 mol % guanine plus cytosine. LKT-1 appears only distantly related to physiologically similar bacteria. A new genus Acetogenium is proposed, and the species is Acetogenium kivui.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 127 (1980), S. 87-97 
    ISSN: 1432-072X
    Keywords: Chloroflexus aurantiacus ; Chlorosomes ; Cytoplasmic membranes ; Bacteriochlorophyll ; Cardenoids ; Pigment-protein complexes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The development of chlorosomes and their pigmentation were studied by growing Chloroflexus aurantiacus strain Ok-7o-fl first under conditions under which BChl c-synthesis is low (50°C, 2000 lux and 30°C, 1500 lux) and subsequently under conditions promoting high BChl c-synthesis (50°C, 400 lux). Electron microscopic observations on and chemical analyses of isolated cell components showed that in BChl c-depleted cells chlorosome-like structures (“chlorosome bags”) are attached to fragments of cytoplasmic membranes. These chlorosome bags exhibit a periodic fine structure caused by the construction of the baseplates of the chlorosomes. The baseplates are closely attached to the cytoplasmic membrane, they are rich in phospholipids and apparently contain a 790 nm-BChl a-complex. Chlorosome bags of BChl c-depleted cells always contain a limited amount of light-harvesting pigment complexes (BChlc, γ- and β-carotene). The light-harvesting system is restored (50°C, 400 lux) by first refilling the existing chlorosome bags before cell division takes place.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-072X
    Keywords: Methanococcus jannaschii ; Thermophilic methanogenic bacteria ; Methanococcus ; Hydrothermal vent methanogen ; Deep sea methanogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new extremely thermophilic methane-producing bacterium was isolated from a submarine hydrothermal vent sample collected by a research team from the Woods Hole Oceanographic Institution using the manned submersible ALVIN. The sample was obtained from the base of a “white smoker” chimney on the East Pacific Rise at 20° 50′ N latitude and 109° 06′ W longitude at a depth of 2600 m. The isolate was a motile irregular coccus with an osmotically fragile cell wall and a complex flagellar system. In defined medium with 80% H2 and 20% CO2, the isolate had a doubling time of 26 min at 85° C. The pH range for growth was 5.2 to 7.0 with an optimum near 6.0. NaCl was required for growth with an optimum of 2 to 3% (w/v). The mol % G+C was 31%. In cell-free extracts, methane formation from methylcoenzyme M was temperature-dependent, and H2 or formate served as electron donors. Methane formation from H2 and CO2 occurred at a much lower rate. Oligonucleotide cataloging of the 16S ribosomal RNA established the isolate as a new species of the genus Methanococcus and the name Methanococcus jannaschii is proposed. The isolation of M. jannaschii from a submarine hydrothermal vent provides additional evidence for biogenic production of CH4 from these deep-sea environments.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-072X
    Keywords: Anabaena sp. ; Nitrogenase ; H2-inhibition ; Photosynthesis ; Pigment concentration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Molecular hydrogen strongly inhibits C2H2-reducing activity in intact cells of Anabaena sp. TA 1 in the light under anaerobic conditions. The inhibitory effect can be partially relieved by supplying the cells with molecular oxygen. When cells of Anabaena sp. TA 1 were grown under anaerobic N2-fixation conditions in the presence of 0.05 bar H2 with white light a pronounced decrease of growth rate occurred. With ammonium ions as nitrogen source the inhibitory effect of H2 was less pronounced. Concomitant with the reduced growth under N2 in the presence of H2 a loss of blue-green pigmentation was observed due to a diminished phycocyanin content. On the other hand, the concentration of carotenoids and chlorophyll remained nearly constant. With NH 4 + as nitrogen source nearly no alteration of phycocyanin content occurred upon incubation with H2. In addition, H2 induced a random distribution of thylakoid membranes in vegetative cells which normally exhibited a curved, parallel pattern. In heterocysts, however, photosynthetic membranes were always arranged randomly. Under far red light, growth and activity of photosystem II were largely diminshed. Under these conditions H2 exhibits an additional inhibitory effect. However, compared to 62% growth inhibition under white light, a decrease of only 20% occurred. Measurements of the photosynthetic electron flow with isolated thylakoid membranes showed that oxidation of diphenylcarbazide (DCP) by membranes from H2-grown cells was inhibited by 28% compared to membranes from control cells. Using ascorbate/DCPIP as electron donor an inhibition of only 1–4% was measured. It is concluded, that H2 inhibits electron flow in the photosynthetic electron transport chain at a site between photosystem II and photosystem I.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-0649
    Keywords: 42.65 ; 07.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 32 (1960), S. 155-163 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Jeder technische Scheidungsvorgang, der sich an einer Körnung vollzieht, ist eine Summe von Teilungsvorgängen, die innerhalb eines gewissen Eigenschaftsbereiches (Trennfehlerzone) stattfinden. Die verschiedenen Scheidungsvorgänge verlaufen nach einem bisher nur angenähert bekannten statistischen Gesetz, das sich in einem S-förmigen Verlauf der sog. Teilungs-Kurve (T-Kurve) manifestiert. Diese Kurven haben nur einen begrenzten Geltungsbereich, der durch die Trennbarkeit des Rohstoffes sowie durch betriebliche Bedingungen (z. B. Überlastung) eingeschränkt wird. Diese Begrenzung vermindert jedoch keineswegs den Wert der T-Kurve, weil die Apparate für die verschiedenen Scheidungsvorgänge praktisch nur für diesen Bereich ausgelegt sind. Wird dieser Bereich überschritten, dann verändert sich die Form der T-Kurve in charakteristischer Weise. Aus solchen Veränderungen kann der erfahrene Praktiker auf ihre Ursachen schließen.
    Additional Material: 22 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 21 (1888), S. 1566-1572 
    ISSN: 0365-9496
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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