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  • 1980-1984  (3)
  • 1945-1949
  • Chemistry  (2)
  • SDS-gel electrophoresis  (1)
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  • 1
    ISSN: 1432-0584
    Keywords: Pyruvate kinase deficiency ; Allosteric regulation ; SDS-gel electrophoresis ; Erythrocyte glycolytic intermediates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A new PK variant with moderate hemolytic anemia is described. The enzymes of the nonanemic parents show sigmoidal reaction kinetics, with normal kinetic parameters, but differ with respect to nucleotide specificity, thermostability, and the concentrations of the glycolytic intermediates in the erythrocytes. The most characteristic features of the patient's (daughter) enzyme are a 30% activity, hyperbolic reaction kinetics and only two bands in the SDS-gel electrophoresis instead of three bands observed with the parental enzymes. Moreover, the pH-optimum is shifted to the acidic range, the affinity for PEP and ADP is decreased, ATP inhibition is negligible and FDP-activation is roughly ten times smaller than with controls. The concentrations of 2.3-DPG, 2-PG and PEP in the erythrocyte are increased, but ATP decreased. As there is no consanguinity in the parents and their enzymes are different this PK mutant can be considered to be compound-heterozygous for two different mutant PK alleles.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 15 (1984), S. 246-250 
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Schallemissionsprüfung von Wärmetauschern des Reaktors SaphirDie Schallemissionsanalyse ist eine zerstörungsfreie Prüfmethode, die zur Untersuchung und Überwachung von Strukturen und druckführenden Komponenten verwendet wird. Das Ziel der Messung am 5 MW Forschungsreaktor Saphir war die Untersuchung und Auswertung der hohen Betriebsgeräusche in den Wärmetauschern des Kühl-systems.Die Ergebnisse zeigten, daß von den 9 Wärmetauschern, die in 3 Gruppen angeordnet sind, 5 Wärmetauscher ein hohes Betriebsgeräusch aufweisen. Die Ursache ist auf die Schwingung der Rohrbündel in Wärmetauschern beim Durchfluß des Pool-Wassers zurückzuführen. Die axiale Ortung bestätigte, daß die Geräusche von mehreren Quellen verursacht werden.
    Notes: The acoustic emission technique is a non-destructive testing method used for inspection and surveillance of structures and pressurized components. In particular, the purpose of the measurement on the 5 MW research reactor SAPHIR was to investigate and evaluate the abnormally high noise levels during operation of the heat exchangers of the cooling system.The results showed that 5 heat exchangers out of the total of 9 (assembled in 3 groups) were responsible for the higher noise level. This is probably due to some abnormal vibration of the tube bundles therein, caused by the through-flow of the pool water. Indeed, attempts of localizing the source showed that there must be several, distributed sources.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0323-7648
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: The relationships between charge carrier transport and breakdown mechanism are summarily discussed and essential aspects of the electric breakdown in solid polymer insulating materials, particularly the effect of defects on the field strength at breakdown and the life time characteristics, are considered. The most important polymers applied in cable insulation are characterized with respect to their parameters and their application facilities are explained.
    Notes: Die Zusammenhänge zwischen Ladungstransport- und Durchschlagmechanismen werden zusammenfassend eingeschätzt und wesentliche Aspekte des elektrischen Durchschlagverhaltens polymerer Feststoffisolierungen dargestellt. Dabei wird besonders auf den Einfluß von Fehlstellen, auf die Durchschlagfeldstärke und die Lebensdauerkennlinien eingegangen. Die für den Einsatz in der Kabelisoliertechnik wichtigsten Polymere werden bezüglich ihrer Materialkenndaten charakterisiert und ihr Einsatz in der Kabeltechnik erläutert. Ferner werden die Kenndaten wichtiger polymerer Dielektrika und die Vorteile und Grenzen ihres Einsatzes als Kondensatorfolie zusammengefaßt.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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