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  • 1980-1984  (4)
  • Cell & Developmental Biology  (4)
  • Key words Dual AV nodal conduction – double ventricular responses – slow pathway radiofrequency ablation
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  • Cell & Developmental Biology  (4)
  • Key words Dual AV nodal conduction – double ventricular responses – slow pathway radiofrequency ablation
  • Life and Medical Sciences  (4)
  • 1
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Prolactin and GH cells were identified in thin sections taken from the adenohypophyses of adult male (6) and female (8) white-tailed deer, collected during all seasons of the year, by locating portions of the same cells in adjacent thick plastic sections immunostained for either PRL or GH. Growth hormone cells were round to ovoid in shape and filled with dense spherical secretory granules which ranged in size from 168 to 682 nm, with a mean diameter of 367 ± 18 nm (x̄ ± SD) in the 14 glands studied. No apparent seasonal changes were evident in the ultrastructural appearance of GH cells. Prolactin cells were small and angular in shape during the winter months and contained only a few small secretory granules. By early summer, they were markedly hypertrophied, round to oval in shape, and densely packed with large spherical secretory granules. The increase in size of PRL secretory granules was most prominent in pregnant females in May, when their mean diameter was approximately double that seen in midwinter. The ultrastructural appearance of PRL cells in September was similar to that of cells studied in March. The size distribution of PRL secretory granules overlapped that of the GH granules, ranging from 114 to 564 nm, and the mean diameter was 246 ± 53 nm, calculated from all 14 individual glands. Our observations suggest that the synthesis and secretion of PRL are closely linked to photoperiodic changes in this species, and they demonstrate the necessity of using specific immunocytochemical techniques in the ultrastructural identification of pituitary acidophils, and of specifying the time of year and location (species photoperiod) in studies concerning PRL physiology, particularly when dealing with wild animal populations.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 168 (1983), S. 239-256 
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The several cell types in mouse and rat rectosigmoid colon have been examined with light and electron microscopic methods for localizing and characterizing complex carbohydrates. Mucous cells, also termed vacuolated cells, and goblet cells comprised most of the deep crypt epithelium in both species, and absorptive columnar cells and goblet cells mainly populated the more superficial epithelium of the upper crypts and main lumen. Occasional tuft cells and enteroendocrine cells were also encountered.Transitional cells structurally intermediate between mucous cells and absorptive cells contained granules characteristic of mucous cells and vesicles like those of columnar absorptive cells. These intermediate cells supported the concept of replacement of mucous by absorptive cells through transformation of mucous into absorptive cells. The intermediate cells also contained numerous lysosomes often in apparent fusion with mucous granules, indicating crinophagic disposal of mucous granules as a mechanism in the cell transformation. Glycoconjugate in absorptive cell vesicles resembled that coating the apical plasmalemma and appeared to represent the source of the glycocalyx of the brush border. Complex carbohydrate in these vesicles differed cytochemically from that of the mucous cell granules, which release their content into the crypt lumen. The absorptive cell vesicles, therefore, constitute an organelle distinct from the mucous cell granules rather than an atrophic form of the latter in a more mature cell.Goblet cells differed in failing to transform morphologically with age but changed in the cytochemical characteristic of their secretion during migration up the crypts. Terminal N-acetylglucosamine residues diminished, while terminal sialic acid-galactose dimers increased during the upward migration, indicating activation of glycosyl transferase synthesis in relation to goblet cell maturation.Glycoconjugate in secretion of mucous cell granules differed markedly from that in goblet cell granules, and content of both organelles differed from that of absorptive cell vesicles. However, secretion in mucous cell granules appeared generally similar for mice and rats with minor exceptions, and secretion in goblets of mice generally resembled that in goblets of rats. Cells interpreted tentatively as Kulchitsky cells stained for high content of fucose with the Ulex europeus I lectin. Globoid leukocytes infiltrating the epithelium of the rat but not the mouse rectosigmoid colon resembled globoid leukocytes in rat tracheal epithelium and, like the latter, appeared to derive from mast cells.
    Additional Material: 21 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 168 (1983), S. 345-362 
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Paraffin sections of mouse and rat kidney were stained with a battery of ten lectin-horseradish peroxidase conjugates and lectin binding was correlated with the ultrastructural distribution of periodate-reactive sugar residues as determined by the periodic acid-thiocarbohydrazide-silver proteinate technique. Various segments of the uriniferous tubule in both species showed differential affinity for labelled lectins. Significant differences were also evident between comparable tubular segments in mouse and rat kidneys. Neutral glycoconjugates containing terminal β-galactose and terminal α-N-acetylgalactosamine were prevalent on the luminal surface of the proximal convoluted tubule in the rat, but α-N-acetylgalactosamine was absent in this site in the mouse. In both species, terminal N-acetylglucosamine was abundant in the brush border of proximal straight tubules but absent in proximal convolutions. Fucose was demonstrated in both proximal and distal segments of mouse kidney tubules but only in the distal nephron and collecting ducts in the rat. Lectin staining revealed striking heterogeneity in the structure and distribution of cellular glycoconjugates. Such cellular heterogeneity was previously unrecognizable with earlier histochemical methods. The marked cellular heterogeneity observed with several lectin-conjugates in distal convoluted tubules and collecting ducts of both species raises a prospect that lectins can provide specific markers for intercalated and principal cells in the mammalian kidney. Glycoconjugates containing terminal sialic acid and penultimate β-galactose were present on vascular endothelium in both rodent kidneys, as were terminal α-galactose residues; but both species lacked reactivity for Ulex europeus I lectin in contrast to human vascular endothelial cells. The constant binding pattern of lectin conjugates allows convenient and precise differentiation of renal tubular segments and should prove valuable in the study of changes in kidney morphology promoted by experimental manipulation or pathologic changes.
    Additional Material: 22 Ill.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Numerous investigators have shown a relationship between basal serum prolactin (PRL) levels and photoperiod in domestic ungulates, and we have shown recently that a similar seasonal baseline serum PRL profile exists in both sexes of a captive but free-breeding population of white-tailed deer. In order to localize and describe the distribution and possible seasonal changes of PRL and growth hormone (GH) cells in the adenohypophyses of deer, we have used antisera raised against ovine PRL and bovine GH and the unlabeled antibody enzyme technique, in conjunction with point counting morphometry. In addition, we have measured seasonal changes in pituitary PRL concentrations in this same population, using a heterologous application of a recently developed homologous radioimmunoassay (RIA) for ovine PRL. Our results show that GH and PRL cells comprise two morphologically separate and immunologically distinct populations. Growth hormone cells were distributed evenly throughout the gland, except in the zona tuberalis, and males had a slightly greater volume density of immunoreactive GH than females; however, there were no differences between sexes in the average size of these cells, and little seasonal change was observed in these cell populations. In contrast to GH cells, the volume density of PRL cells, the size of PRL cells, and the pituitary concentration of RIA-measurable PRL all showed marked seasonal changes in deer of both sexes. These changes were well correlated with one another and with previously reported changes in basal serum levels (in these animals), in that they all reached zenith during midsummer, and nadir in midwinter. Our results demonstrate the necessity of reporting the time of year and latitude (to specify photoperiod) in studies concerning PRL cell physiology, particularly when dealing with any wild animal population.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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