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  • 1
    Electronic Resource
    Electronic Resource
    Comparison of Dolichos biflorus lectin and other lectin-horseradish peroxidase conjugates in staining of cutaneous blood vessels in the hairless mini-pig (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 17 (1990), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Angiogenesis is necessary for normal growth, wound healing, and plays a key role in many pathologic processes. A variety of endothelial markers have been used to investigate angiogenesis. Unfortunately, excellent markers for vascular endothelium in human tissues exhibit little or no staining of endothelia in tissues of other animal species, including the pig. We are interested in the hairless Yucatan strain of mini-pig as an animal model for studying cutaneous wound healing because its skin is histologically and functionally very similar to that of man. Hoping to find a specific marker to identify vascular endothelium in the mini-pig, we therefore screened a battery of 11 different lectin-horseradish peroxidase conjugates. Based on specificity and staining intensity, Dolichos biflorus agglutinin (DBA) was chosen from this battery to investigate vascular changes in the healing of cutaneous wounds in the mini-pig. When compared with routine histologic sections stained with hematoxylin and eosin, blood vessels were much easier to identify in sections stained histochemically with DBA. Lectin histochemistry was particularly useful in investigations of early events in angiogenesis during wound healing when newly derived capillary buds and minute blood vessels were obscured in normal histologic sections by an inflammatory cell infiltrate associated with the healing wound. Ultrastructural lectin cytochemistry revealed staining along the luminal surface and the basolateral plasmalemma of endothelial cells. Histochemical staining with DBA promises to provide a useful method for further investigation of angiogenesis and other vascular phenomena in a variety of normal and pathologic processes using the hairless Yucatan strain of mini-pig as the animal model.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1990.tb01671.x
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  • 2
    Electronic Resource
    Electronic Resource
    Glycoconjugate with terminal α galactose (1986)
    Springer
    Histochemistry and cell biology 84 (1986), S. 387-395 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Glycoconjugates associated with the basal cell layer of various types of epithelia in the mouse and rat were examined histochemically with a battery of lectinhorseradish peroxidase (HRP) conjugates of differing sugar binding specificities. Basal cells in paraffin sections of composite tissue blocks stained with an isolectin from Griffonia simplicifolia (GSA I-B4) specific for terminal α-galactose residues but failed to react with the other lectins. Basal cells in epithelium lining striated and excretory ducts of salivary and lacrimal glands, tongue, esophagus, trachea, renal calyx, ureter, urinary bladder, urethra, epididymis and vas deferens stained selectively and intensely for content of a glycoconjugate with terminal α-galactose. This galactoconjugate appeared associated with the plasmalemma of basal cells. Basal cells with a galactocalyx formed an intermittent to continuous layer generally increasing in prevalence distally in glandular duct systems. A minor population of pyramido-columnar cells with cytosolic GSA I-B4 reactivity occurred in striated ducts and appeared less numerous in intralobular excretory ducts and more prevalent in extraglandular ducts. In trachea and renal pelvis, the GSA I-B4 positive cell profiles ranged from low cuboidal to tall pyramidal in contour, but the latter appeared not to reach the lumen. In contrast, no GSA I-B4 positive basal cells were seen in any segment of the pancreatic or bile ducts or in the epithelium of the gastrointestinal tract. These findings suggest that the basal cells found in similar sites in different epithelia and possessing in common a unique α-galactoconjugate may function in a manner common to all and not simply in providing progenitor cells for epithelial renewal. The location and distribution of GSA I-B4 reactive basal cells in diverse epithelia suggests that through their α-galactocalyx they serve in maintaining the established composition of luminal fluid perhaps by impeding the transepithelial movement of fluid and ions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00482968
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  • 3
    Electronic Resource
    Electronic Resource
    Lectin cytochemistry of cell types in human and canine pituitary (1986)
    Springer
    Histochemistry and cell biology 85 (1986), S. 57-66 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Labeled lectins specific for different sugars were employed to identify different cell types in pituitaries from six human autopsies and seven dogs. To determine the lectins bound by each cell type, fixed-paraffin embedded sections serial to those stained with lectins were immunostained for specific hormones and the serial pairs were examined in a comparison microscope. In human pituitaries corticotrophs stained selectively with lectins having affinity for α-l-fucose and the core region of complex type N-glycosyl-proteins. Some corticotrophs also stained for the presence of terminal β-galactose. Thyrotrophs stained selectively with a periodate oxidation-borohydride reduction-concanavalin A sequence. Some mammotrophs evidenced content of glycoconjugate with terminal β-galactose. Dendritic cells stained selectively for abundant glycogen with the periodate-reduction-concanavalin A sequence and a lectin from Griffonia simplicifolia. Adenohypophyseal cells of dog pituitary differed in showing absence of terminal β-galactose in corticotrophs, presence of terminal β-galactose in thyrotrophs, presence of glycoconjugate with N-glycosidically bound oligosaccharide in thyrotrophs and gonadotrophs and presence of terminal β-galactose with a different lectin affinity in mammotrophs. The main contributions of lectin histochemistry applied to the pituitary include providing an additional histologic method for identification of some cell types, and localizing glycosylated prohormone or other biochemically unrecognized non-hormone glycoconjugates whose function in pituitary cells remains to be explained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00508654
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  • 4
    Electronic Resource
    Electronic Resource
    Glycoconjugate with terminal galactose (1989)
    Springer
    Histochemistry and cell biology 91 (1989), S. 61-67 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pulmonary macrophages in pre- and postnatal rats were examined histochemically with a battery of peroxidase labeled lectins. Among them, Griffonia simplicifolia agglutinin I-B4 (GSA I-B4) which binds specifically to terminal α-galactose showed selective affinity in lung for the monocyte-macrophage line. These cells were demonstrable with GSA I-B4 from the 14th day of gestation through the adult. Extension to the ultrastructural level showed strong selective binding of this lectin to the surface of the plasmalemma and inner face of membranes limiting phagosomes in macrophages. At day 14 of gestation, monocytelike cells positive with GSA I-B4 were scattered in various organs including lung. The lectin reactive cells in lung increased in number and size with development, infiltrating the interstitium through day 20 of gestation and then also entering the alveolar space. These findings suggest that GSA I-B4 recognizes a surface glycoconjugate characteristic of the pulmonary monocyte-macrophage line. Such selective lectin affinity offers a marker for detecting the pulmonary macrophages and examining their kinetics by light and electron microscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00501913
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  • 5
    Electronic Resource
    Electronic Resource
    The influence of embedding media and fixation on the post-embedment ultrastructural demonstration of complex carbohydrates (1983)
    Springer
    Histochemistry and cell biology 79 (1983), S. 417-431 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The influence of fixation and the embedding medium on post-embedment dialyzed iron (DI) staining of acidic complex carbohydrates in mouse colon was studied at the ultrastructural level. DI staining of ultrathin sections from non-osmicated tissues embedded in epoxy resins was very weak, whereas DI staining of non-osmicate tissues embedded in non-epoxy resins such as polystyrene, polyester resins, methacrylates and newly developed embedding mixtures was strong. Brief exposure to OsO4 (5 min) abolished the DI staining in stored secretions of goblet and mucous cells, in apical cytoplasmic vesicles and at the microvillous surface of columnar absorptive cells and in the Golgi cisternae of all colonic cell types in epoxy embedded tissues, but only reduced slightly or had no effect on the DI reactivity observed in these sites in tissues embedded in non-epoxy resins. Prolonged exposure to OsO4 (60 min) prior to embedment in non-epoxy resins further reduced DI staining in all reactive sites and abolished the staining in Golgi cisternae of all colonic epithelial cells. Embedment of non-osmicated tissues in a styrene-Vestopal W mixture and of tissues briefly exposed to OsO4 after primary glutaraldehyde fixation in styrene-methacrylate is recommended for optimal post-embedment DI staining of the acidic groups of complex carbohydrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00491777
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  • 6
    Electronic Resource
    Electronic Resource
    Light microscopic detection of sugar residues in glycoconjugates of salivary glands and the pancreas with lectin-horseradish peroxidase conjugates. I. Mouse (1983)
    Springer
    Journal of molecular histology 15 (1983), S. 1217-1238 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mouse salivary glands and pancreases were stained with a battery of ten horseradish peroxidase-conjugated lectins. Lectin staining revealed striking differences in the structure of oligosaccharides of stored intracellular secretory glycoproteins and glycoconjugates associated with the surface of epithelial cells lining excretory ducts. The percentage of acinar cells containing terminal α-N-acetylgalactosamine residues varied greatly in submandibular glands of 30 male mice, but all submandibular acinar cells contained oligosaccharides with terminal sialic acid and penultimate β-galactose residues. The last named dimer was abundant in secretory glycoprotein of all mucous acinar cells in murine sublingual glands and an additional 20–50% of these cells in all glands contained terminalN-acetylglucosamine residues. In contrast, terminal α-N-acetylgalactosamine was abundant in sublingual serous demilune secretions. Serous acinar cells in the exorbital lacrimal gland, posterior lingual gland, parotid gland and pancreas exhibited a staining pattern unique to each organ. In contrast, the apical cytoplasm and surface of striated duct epithelial cells in the submandibular, sublingual, parotid and exorbital lacrimal gland stained similarly. A comparison of staining with conjugated lectins reported biochemically to have very similar carbohydrate binding specificity has revealed some remarkable differences in their reactivity, suggesting different binding specificity for the same terminal sugars having different glycosidic linkages or with different penultimate sugar residues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01002741
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  • 7
    Electronic Resource
    Electronic Resource
    The influence of embedding media and fixation on the post-embedment ultrastructural demonstration of complex carbohydrates. I. Morphology and periodic acid-thiocarbohydrazide-silver proteinate staining of vicinal diols (1983)
    Springer
    Journal of molecular histology 15 (1983), S. 763-784 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The influence of fixation and embedding medium on the periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) staining reactivity in the mouse intestine was studied. It was found that the combination of osmium tetroxide and epoxy resins was the least sensitive for the demonstration of complex carbohydrate with the PA-TCH-SP method. Post-osmication reduced, but did not abolish, PA-TCH-SP reactivity (except for the Golgi complex) when non-epoxy resins were used. The staining pattern of a particular organelle differed depending on the embedding medium used. Golgi cisternae exhibited the most intense PA-TCH-SP reactivity in non-osmicated tissues embedded in non-epoxy resins. Post-osmication of tissues was required to reveal the fine structure of the glycocalyx as well as to preserve the fine structure of tissues embedded in styrene-methacrylate and styrene-Rigolac 2004. The choice of fixation procedures and embedding media in a given study should be governed primarily by the sites of interest.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01003340
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  • 8
    Electronic Resource
    Electronic Resource
    Lectin histochemistry of nephroblastoma (Wilms' tumour) (1985)
    Springer
    Journal of molecular histology 17 (1985), S. 1091-1110 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Paraffin sections of seven cases of nephroblastoma and one case of clear cell sarcoma were stained with a battery of eleven lectins conjugated to horseradish peroxidase. Lectin staining revealed similarities between blastema and stroma with respect to their content of glycoconjugates whereas blastema and epithelial cells exhibited major differences. In general, blastema and stroma contained glycoconjugates with terminal or penultimate β-galactose, glycoconjugates having either biantennary or triantennary N-linked sugar chains or both, sialoglycoconjugates, and occasionally glycogen. Epithelial cells also showed these complex carbohydrates but stained additionally for terminal disaccharide galactose-(β1→3)-N-acetylgalactosamine, terminal α-galactose and terminal α-N-acetylgalactosamine. Furthermore, staining with three fucose-binding lectins revealed that the linkage between terminal α-fucose residues to the constituent oligosaccharide chains varied between epithelial cells, blastema and stroma. In general, the distribution and content of glycoconjugates in tumour cells comprising clear cell sarcoma resembled that in blastema and stroma of nephroblastoma. Other findings included differences in content of glycosubstance between cuboidal and columnar cells within the same tumour. Also observed were variations between a primary tumour and its metastasis with respect to the occurrence of certain complex carbohydrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01002535
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  • 9
    Electronic Resource
    Electronic Resource
    Glycoconjugate unique to migrating primordial germ cells differs with genera (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 228 (1990), S. 177-184 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Previous cytochemical studies showing that rat primordial germ cells (PGCs) possess a unique surface glycoconjugate containing terminal α-Nacetylgalactosamine were extended in this study to determine whether a similar distinctive glycoconjugate coats the surface of PGCs in the mouse. The results showed that mouse PGCs fail to react with peroxidase-conjugated lectins specific for localizing glycoconjugate with terminal N-acetylgalactosamine. All available lectin conjugates with affinity for other terminal sugars or internal sugar linkages also failed to stain mouse PGCs except for the conjugates that bind to α-fucose. One fucose-specific lectin conjugate stained only PGCs in the early mouse embryo but stained additional sites in more mature embryos and lost reactivity with PGCs after gestational day 14. Another fucose-specific conjugate stained PGCs until day 15, but with less selectivity, and a third such conjugate bound to several sites, but not to PGCs. The results suggest that the developmental mechanisms mediating cellular interaction, migration, and differentiation may be similar in different genera, but the specific structure of the cell surface glycoconjugate involved in these mechanisms differs.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092280209
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  • 10
    Electronic Resource
    Electronic Resource
    Histochemical localization of sialoglycoconjugates with a sialic acid-specific lectin from the slugLimax flavus (1984)
    Springer
    Journal of molecular histology 16 (1984), S. 1125-1132 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01002899
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