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  • 1
    Electronic Resource
    Electronic Resource
    Depression of reserpine-induced muscular rigidity in rats after administration of lisuride into the spinal subarachnoid space (1980)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 315 (1980), S. 177-179 
    Details
    ISSN: 1432-1912
    Keywords: Lisuride ; Reserpine-induced rigidity ; Spinal cord ; Subarachnoid space
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Muscular rigidity was induced by reserpine (10 mg/kg) in rats and the tonic activity of the gastrocnemius muscle was recorded in the electromyogram. Systemic administration of lisuride, an ergoline, resulted in a dose-dependent depression of rigidity. To examine the spinal cord as a site of action for lisuride to depress reserpine-induced rigidity, a method for the chronic catheterization of the lumbar spinal subarachnoid space was used, which allowed the administration of drugs to reserpinized, intact rats without anaesthesia. Lisuride injected into the lumbar spinal subarachnoid space resulted in a longlasting depression of rigidity. These results suggest that the spinal cord is an important site of action of lisuride.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499261
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Clonale Nervenzellinien in der Kultur — Modelle zum Studium molekularer Grundlagen neuropharmakologischer Wirkungen (1983)
    Springer
    Journal of molecular medicine 61 (1983), S. 1-16 
    Details
    ISSN: 1432-1440
    Keywords: Clonal cell lines ; Differentiation and maturation ; Regulation of neurotransmitter synthesis by drugs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Nerve cell lines with stable properties are isolated from neuroblastomas, glioblastomas or pheochromocytomas by periodic cloning using defined culture media. After the action of different drugs, these cells show all morphological and biochemical signs of differentiation and maturation. Depending on the origin of the clone, the cell lines synthesise typical neurotransmitters, which are stored in vesicles. It is demonstrated on cell lines which synthesise catecholamines that noradrenergic and dopaminergic clones are particularly suitable test objects for basic research in neuropharmacology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01484434
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Further studies on the nature of red fluorescent structures in neuroblastoma monolayer cells vitally stained with acridine organge (1980)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 311 (1980), S. 53-59 
    Details
    ISSN: 1432-1912
    Keywords: Neuroblastoma cells ; Acridine orange ; Golgi derived vesicles ; Ultrastructural studies ; Cytofluorometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The nature of red fluorescent particles in vitally acridine orange stained C 1300 neuroblastoma monolayer cells was evaluated by electron microscopy, cytofluorometry, cytopharmacological and cell fractionation studies. At the ultrastructural level the distribution of red fluorescent granules correlated with that of the Golgi complex and Golgi derived structures during various stages of differentiation, mitosis, and under colcemid treatment. Cytopharmacological studies revealed that red fluorescence was displaced in a concentration and time dependent manner with the basic drugs chloroquine and quinacrine. Subcellular fractionation studies showed that acridine orange was concentrated in fractions that also contained the highest amount of acid phosphatase and electron dense vesicles. Vital acridine orange staining of neuroblastoma cells in culture can give information on the relationship between Golgi-derived vesicles and cell functions like proliferation and differentiation. The influence of drugs on these processes can be studied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00500302
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  • 4
    Electronic Resource
    Electronic Resource
    Dopa-release from mouse neuroblastoma clone N1E-115 into the culture medium (1982)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 320 (1982), S. 85-89 
    Details
    ISSN: 1432-1912
    Keywords: Apomorphine ; Dopa-release ; Neuroblastoma clone ; Tyrosine hydroxylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Due to a lack of l-Dopa-decarboxylase, the mouse neuroblastoma clone N1E-115 contains a high intracellular Dopa-content compared to a low noradrenaline- and dopamine-content. Because of this decarboxylase deficiency, the N1E-115 clone releases more than 95% of the produced Dopa into the culture medium. After renewal of the culture medium, Dopa production of the cells can be measured by the increase of Dopa in the medium. Dopa production was linear during 2 h and varied from 50–180 μg x mg prot−1 x h−1 between different subcultures. Dopa release into the medium was used as an indirect measure for the tyrosine-hydroxylase activity. Several dopaminergic agonists and antagonists were tested. Dopa production could be blocked dose-dependent by apomorphine (1×10−7−1×10−6M), but not by lisuride hydrogen maleate and bromocryptine. Several dopaminergic and adrenergic antagonists failed to reverse the apomorphine induced blockade of the tyrosine-hydroxylase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00506305
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and characterization of internalized glioma cell membranes (1984)
    Springer
    Cell & tissue research 236 (1984), S. 147-151 
    Details
    ISSN: 1432-0878
    Keywords: Phagocytosis ; Plasma membrane ; C6-glioma cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A method for isolating plasma membranes based on the ability of cultured C6-glioma cells to phagocytize inert material such as polystyrene (latex) beads is described. The beads (Ø 1.1 μm) were incubated for 16 h or 5 h. After several washings and homogenization of the cells, the beads with the surrounding membranes were isolated by use of a sucrose density gradient. The membranes were analyzed morphologically and biochemically. Morphological studies by means of light- and electron microscopy confirmed the intracellular localization of the beads. Enzymatic studies revealed that the specific activity of acid phosphatase decreased with shorter incubation periods (from 268.00±38.56 U x mg protein-1 x min-1 after 16 h to 125.12 ± 9.10 after 5 h), whereas that of Na, K-ATPase showed the opposite trend (3.63±0.41 and 4.73±0.78 μmoles phosphate x mg protein-1 x h-1, respectively), indicating a lesser contamination with lysosomes. The main advantages of this procedure for membrane studies lie in purity and definite orientation (“inside-out”) of the membranes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216524
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    Paper (German National Licenses)
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