ISSN:
1432-1912
Keywords:
Rabbit caudate nucleus
;
Dopamine release
;
Apomorphine
;
Bromocriptine
;
Chlorpromazine
;
Haloperidol
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Effects of dopamine receptor agonists and antagonists on the release of dopamine were studied in the caudate nucleus of the rabbit. The nucleus contained 6.7 μg/g of dopamine, but negligible levels of noradrenaline and dopamine-β-hydroxylase. No formation of 3H-noradrenaline was detected in caudate slices preincubated with 3H-dopamine, and more than 95% of the tritium content of the tissue consisted of 3H-dopamine. When caudate slices were preincubated with 3H-dopamine and then superfused with amine-free medium, there was a basal outflow of tritium that was not or only slightly changed by tetrodotoxin (10−7 and 10−6 M), apomorphine (up to 10−5 M), bromocriptine (up to 10−6 M), chlorpromazine (up to 10−6 M), haloperidol (up to 10−7 M), or omission of calcium. Electrical stimulation (3 Hz, 24 mA, 2 ms pulse duration, 2-min periods) greatly increased the outflow of tritium. The stimulation-evoked overflow was abolished by tetrodotoxin (10−7 and 10−6 M) and in calcium-free medium. Apomorphine (10−8–10−5 M) and bromocriptine (10−8–10−6 M) reduced, whereas chlorpromazine (10−7 and 10−6 M) and haloperidol (10−8 and 10−7 M) enhanced the evoked overflow. The inhibitory effect of apomorphine and bromocriptine was antagonized by chlorpromazine and haloperidol, but not by phentolamine. Silicone tubings that had been in contact with 3H-haloperidol retained tritiated material that was slowly eluted during perfusion with water or physiological salt solution. The material was identified as 3H-haloperidol. When silicone tubings pretreated with unlabelled haloperidol were used in subsequent dopamine release experiments, the inhibitory effect of apomorphine was not reproduced. It is concluded that, in the caudate nucleus of the rabbit, apomorphine and bromocriptine depress, whereas chlorpromazine and haloperidol facilitate action potential-evoked release of dopamine. The effects are mediated by specific receptors which may be located on the dopaminergic nerve terminals. The receptors appear to be normally activated by released dopamine itself, which thus inhibits its own further release. Part of the discrepancies in the literature concerning dopaminergic modulation of dopamine release may be due to retention of neuroleptic drugs in superfusion assemblies, followed by slow elution and interference with subsequent experiments.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00497003
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