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  • 1975-1979  (2)
  • 1960-1964
  • 1930-1934
  • Chemistry  (1)
  • Filtration leukapheresis  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 56 (1978), S. 409-414 
    ISSN: 1432-1440
    Keywords: Filtrationsleukapherese ; Granulozyten ; Phagozytose ; Intrazelluläre Keimabtötung ; Chemotaxis ; Filtration leukapheresis ; Granulocytes ; Phagocytosis ; Intracellular microbicidal activity ; Chemotaxis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A simple continuous filtration leukapheresis has been devised whereby two Leuko-Pak filters can be charged and eluted within 5 h with approximately 12 l of blood in a closed system of tubes. Blood flows by means of gravity without the use of pumps. Without premedication with prednisolone the average yield from 10 donors was 3.66×1010 granulocytes per leukapheresis and after premedication with 150 mg prednisolone orally it was 6.44×1010 granulocytes per leukapheresis. The in vitro function of filter granulocytes was not reduced even after premedication with prednisolone. In comparison with leukapheresis by blood cell separators this modified method of filtration leukapheresis offers the following advantages: 1) the granulocyte yield per litre of whole blood processed is higher, 2) technical assistants in a blood-bank can carry out the method on several donors simultaneously, 3) the method is more economical than leukapheresis by blood cell separators.
    Notes: Zusammenfassung Es wurde eine einfache kontinuierliche Filtrationsleukapherese (FL) zur Gewinnung von Granulozyten entwickelt, mit der zwei Leuko-Pak-Filter innerhalb von 5 h mit etwa 12 Liter Blut im geschlossenen System beschickt und eluiert werden können. Der Blutfluß erfolgt dabei ohne Pumpe allein durch Schwerkraft. Die durchschnittliche Ausbeute betrug bei 10 Spendern ohne Vorbehandlung mit Prednisolon 3,66×1010 Granulozyten pro Leukapherese, nach Vorbehandlung mit 150 mg Prednisolon p.o. 6,44×1010 Granulozyten pro Leukapherese. In vitro-Tests zeigten, auch nach Gabe von Prednisolon, keine Einschränkung der Funktionsfähigkeit der Filtergranulozyten. Die vorliegende modifizierte Form der FL hat im Vergleich zur Leukapherese durch Blutzellseparatoren folgende Vorteile: 1. Die Granulozytenausbeute bezogen auf Liter separiertes Vollblut ist höher. 2. Die Methode kann durch Hilfspersonal in einer Blutbank an mehreren Spendern gleichzeitig durchgeführt werden und sie ist 3. wirtschaftlicher als die Leukapherese mit Blutzellseparatoren.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 18 (1976), S. 891-907 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: There are four main sources of enzymes in foods - these being the inherent enzymes, enzymes from microbial contaminants, enzymes elaborated by microorganisms added to foods, and specific enzymes added to foods. This study primarily deals with the latter two sources of enzymes in food. Although both plants and animals serve as sources of enzymes, they are not as economical or versatile sources as are enzymes obtained from microorganisms. In the meat industry, proteases are used to tenderize muscle and to obtain flavor precursors. In the preparation of cured meat products such as sausages, lipases, and proteases from bacterial cultures are utilized. Similarly, proteases and lipases are used in the dairy industry to develop flavor compounds. Proteases and amylases also have applications in the baking and milling industries where they are used to produce precursors for the nonenzymatic browning reactions. Carbohydrases such as amylase, amyloglucosidase, and glucose isomerase have found usage in the starch and syrup industry for the production of high dextrose and high fructose syrups. Other enzymes such as glucose oxidase, pectinase, and naringinase are of value to the wine and fruit juice industries. A better understanding of the mode of action of enzymes as well as the mechanisms of development of flavor compounds will further enhance the use of microbial enzymes to develop specific and desired flavors in foods.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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