ISSN:
1432-2013
Keywords:
Single Nephron
;
Glomerular Filtration Rate
;
Microsphere Distribution
;
Microdissection
;
Extra Cellular Fluid Volume Expansion
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary In control non diuretic (ND) and in salt-loaded (SL) rats, both the microsphere technique and the14C ferrocyanide infusion technique were used to determine the distribution of microspheres in single glomeruli and the SNGFR of the corresponding nephrons. A sample of microspheres with a diameter averaging 11.0±2.6 μ SD was selected from a 15±5 μ unlabelled batch. In each rat, three million of these microspheres were injected through the left carotid artery. The microspheres were directly counted under microscopic observation in the glomeruli of the nephrons which were microdissected to determine the SNGFR value. The number of microspheres per glomerulus for a given kidney generally varied from 0 to 8 and was independant to the SNGFR value. The diameter of the microspheres trapped was constant in all the animals. The mean number of microspheres for superficial (S) and juxtamedullary (JM) nephrons was, (ND):S=1.99±0.48 SE.,n=5; JM=3.02±0.51 SE,n=5;P〈0.02, (SL):S=3.75±0.53 SE,n=6; JM=2.86±0.33 SE,n=6;P〈0.05. This distribution was directly related to that of SNGFR in ND rats (S=39.0±6.1 SD and JM=49.5±10.3 nl/min) but not in SL rats (S=50.9±6.1 and JM=66.9±10.0 nl/min). In conclusion, the microsphere technique described in the present paper, appears more suitable for investigating single glomerular blood flow since the number and the size of the microspheres trapped are directly determined at the level of the glomerulus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00585983
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