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  • Column liquid chromatography  (2)
  • Immunoblot  (2)
  • (S. cerevisiae)  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Purification and properties of multiple molecular forms of yeast peptidyl prolyl cis-trans isomerase
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular 1161 (1993), S. 161-167 
    Details
    ISSN: 0167-4838
    Keywords: (S. cerevisiae) ; Cyclophilin ; Cyclosporine A ; Multiplicity ; Peptidyl prolyl cis-trans isomerase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4838(93)90209-A
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    IgA antikeratinocyte surface autoantibodies from two types of intercellular IgA vesiculopustular dermatosis recognize distinct isoforms of desmocollin (1996)
    Springer
    Archives of dermatological research 288 (1996), S. 447-452 
    Details
    ISSN: 1432-069X
    Keywords: Key words Autoantibody ; Desmocollin ; Immunoblot ; IgA ; Pemphigus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We previously proposed the term intercellular IgA vesiculopustular dermatosis (IAVPD) for cases showing IgA antikeratinocyte surface autoantibodies. This condition is divided into two subtypes: intraepidermal neutrophilic IgA dermatosis (IEN), showing pustule formation in the entire epidermis, and subcorneal pustular dermatosis (SPD) showing pustule formation in the uppermost epidermis. We have previously reported that serum from certain IAVPD patients reacts with bovine desmocollin (Dsc), a desmosomal cadherin. In this study we showed that two Dsc isoforms with a slightly different molecular weight were recognized by the serum from these patients. Further analysis revealed that serum from patients with the SPD type, which stained the cell surface in the uppermost epidermis with immunofluorescence, seem to react with Dsc1. By contrast, serum from patients with the IEN type, which stained the cell surface at all levels of the epidermis with immunofluorescence, seemed to react with Dsc3. This difference of distribution between the two distinct Dsc molecules may contribute to the different clinicopathological features between the IEN and SPD type of IAVPD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004030050081
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The majority of bullous pemphigoid and herpes gestationis serum samples react with the NC16a domain of the 180-kDa bullous pemphigoid antigen (1996)
    Springer
    Archives of dermatological research 288 (1996), S. 507-509 
    Details
    ISSN: 1432-069X
    Keywords: Key words Autoantigen ; Basement membrane zone ; Bullous pemphigoid ; Immunoblot ; Recombinant protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A series of previous studies have indicated that the NC16a domain of the 180-kDa bullous pemphigoid antigen (BP180) is the most immunogenic and probably pathogenic region in both bullous pemphigoid and herpes gestationis. To confirm these previous results by a large-scale study, we examined serum from 154 bullous pemphigoid and 43 herpes gestationis patients using an immunoblot technique with the BP180 NC16a domain fusion protein, which had been prepared using cDNA obtained from a human keratinocyte cDNA library by polymerase chain reaction amplification. This fusion protein was recognized by 90% of bullous pemphigoid and 79% of herpes gestationis serum samples, but not by any other disease or normal control serum samples. These results indicate that this system is very sensitive and specific for the detection of anti-BP180 antibodies and should be useful for the diagnosis of various subepidermal bullous diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004030050093
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Interconversion of cyclosporin molecular form inducing peak broadening, tailing and splitting during reversed-phase liquid chromatography (1994)
    Springer
    Chromatographia 38 (1994), S. 359-364 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Cyclosporin ; Reversible conversion ; Activation energy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Reversed-phase liquid chromatography of cyclosporin A, a peptide immunosuppressant, at various temperatures produced remarkably different chromatograms. At 60°C one sharp peak was obtained, at 23°C this became a single broad peak and between 15° and 0°C this became one high sharp peak followed by a tailing or low plateau. Remarkably different chromatograms were produced also by varying the mobile phase flow-rate. The effects of both temperature and flow-rate on the chromatogram could be accounted for by interconversion between two forms of the cyclosporin molecule. Kinetic analysis showed that one form was converted by first-order kinetics with a half-life of 2.0 min at 20°C and that the apparent activation energy for the conversion was about 18 kcal/mol. The other two immunosuppressants, cyclosporin C and D, were also shown to undergo interconversion. This kinetic analysis of the interconversion should be helpful in clarifying the relationship between molecular structure and activity of the immunosuppressants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02269781
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  • 5
    Electronic Resource
    Electronic Resource
    Fluorometric determination of isoniazid and its metabolites in urine by high performance liquid chromatography (1990)
    Springer
    Chromatographia 30 (1990), S. 69-72 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Isoniazid metabolites in urine ; Fluorometric detection ; Post column derivatization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A rapid, simple, and accurate method was developed for the determination of isoniazid and its metabolites (isonicotinic acid, acetylisoniazid and isonicotinylglycine) in urine by high-performance liquid chromatography. Urine is diluted with the mobile phase. After centrifugation, an aliquot of the supernatant is injected into the chromatograph. Isoniazid and its metabolites are separated by reversed-phase ionpairing chromatography with a mobile phase containing propanesulfonate and detected by fluorometry using postcolumn derivatization at high temperature (150°C) with hydrogen peroxide. The method was applied to the analysis of urine from patients receiving isoniazid therapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02270451
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    Paper (German National Licenses)
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