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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Diabetologia 27 (1984), S. 212-218 
    ISSN: 1432-0428
    Keywords: Protein denaturation ; insulin stability ; automatic delivery devices ; insulin therapy ; polypropylene glycol/polyethylene glycol block polymers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Studies with insulin delivery devices have shown that denaturation of dissolved proteins at hydrophobic interfaces is a basic obstacle to long-term insulin stability in pumps. This study shows that polypropylene glycol/polyethylene glycol block polymers prevent both the adsorption of dissolved proteins to hydrophobic interfaces and the resultant aggregation. At a concentration of 0.001% (w/v), the block polymer Genapol PF-10 stabilises insulin solutions over a wide range of concentrations. Insulin solutions thus stabilised are at present being clinically tested. The effectiveness of molecular variants of Genapol PF-10 to stabilise other proteins (human γ-globulin, myoglobin and serum albumin) is presented also.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1076
    Keywords: Corticosteroide ; 11-β-Hydroxycorticosteroide ; Adrenal cortex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Nach einleitender Diskussion der Methoden zur fluorometrischen Bestimmung der 11-β-Hydroxycorticosteroide im Plasma oder Serum wird eine Mikromethode in Anlehnung an de Moor beschrieben. Von den bisherigen Methoden erfordert sie den geringsten Zeit- und Arbeitsaufwand. Die Zuverlässigkeitskriterien von Mikro- und entsprechender Makromethode sind identisch. Das pro Einzelbestimmung erforderliche Serumvolumen beträgt 0,1–0,3 ml. Abschließend wird die Verwendung der Methode in der pädiatrisch-endokrinologischen Diagnostik diskutiert.
    Notes: Summary Following a discussion of the known methods for the determination of 11-β-hydroxycorticosteroids in plasma or serum by fluorimetry a micro-method is described. The advantages of this technique lie in its requirement for minimal amounts of time and laboratory procedures. The criteria for reliability and specificity of this micro-method are identical with those of the macro-methods. Not more than 0.1–0.3 ml of plasma are required for a single determination. The application of this method in the field of pediatic endocrinology is discussed.
    Type of Medium: Electronic Resource
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