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  • Contact sensitivity  (4)
  • DNCB  (2)
  • 2,4-Dinitrophenylated epidermal cells  (1)
  • 1
    Digitale Medien
    Digitale Medien
    The suppressive effect of tape-stripping treatment of guinea-pig skin on the induction of contact sensitivity by intradermal injection of haptenated epidermal cells (1985)
    Springer
    Archives of dermatological research 278 (1985), S. 102-106 
    Details
    ISSN: 1432-069X
    Schlagwort(e): Tape stripping ; Contact sensitivity ; DNCB ; Dinitrophenylated epidermal cell ; Immunological suppression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Contact sensitivity (CS) to 2,4-dinitrochlorobenzene (DNCB) was produced in inbred JY1-strain guinea pigs by the intradermal injection of epidermal cells (ECs) prepared from DNCB-painted skin (DNP-ECs). When the site of DNP-EC-induced CS was pretreated by tape stripping, the rate and intensity of the challenge reactions to DNCB were diminished. The ability of DNP-ECs to induce CS returned to normal when normal peritoneal macrophages together with DNP-ECs were administered into the stripped skin. Normal ECs had a similar effect. Using either anti-Ia antiserum and complement or allogeneic ECs (strains 2 and 13), Ia-positive cells among the ECs (presumably Langerhans cells) were found to be essential for the recovery of CS. Tape-stripping treatment also resulted in the development of immunological tolerance, as assessed by subsequent painting with a sensitizing dose of DNCB. These findings suggest that the immunological function of the mononuclear-phagocyte system in the dermis may be impaired when the epidermal surface is markedly disturbed by tape-stripping treatment.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00409215
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  • 2
    Digitale Medien
    Digitale Medien
    The distribution of 2,4-dinitrophenyl groups in guinea pigs following feeding of 2,4-dinitrochlorobenzene (1985)
    Springer
    Archives of dermatological research 278 (1985), S. 97-101 
    Details
    ISSN: 1432-069X
    Schlagwort(e): Contact sensitivity ; Tolerance ; Feeding ; DNCB ; Antigen distribution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary An immunofluorescent technique using anti-DNP antibody was employed to investigate the distribution of 2,4-dinitrophenyl (DNP) groups in various tissues following the feeding of 125 mg/kg 2,4-dinitrochlorobenzene (DNCB)-ethanol to guinea pigs that had been starved for 1 day. DNP groups were detected in the areas corresponding to the cytoplasm and the cell membrane of the epithelium in the upper gastrointestinal tract as well as on the cells of mesenteric lymph nodes, Payer's patches, the spleen, and peripheral blood. These results are discussed in relation to the mechanism of tolerance induction produced by the feeding of haptens.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00409214
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  • 3
    Digitale Medien
    Digitale Medien
    Delayed-type skin reaction to 2,4-dinitrophenylated epidermal cells in guinea pigs with contact sensitivity to 2,4-dinitrochlorobenzene (1987)
    Springer
    Archives of dermatological research 279 (1987), S. 236-240 
    Details
    ISSN: 1432-069X
    Schlagwort(e): 2,4-Dinitrochlorobenzene (DNCB) ; Contact sensitivity ; Epidermal cell ; Delayed-type skin reaction ; Langerhans cells
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Contact sensitivity (CS) induced by hapten has been thought to be analogous to delayed-type hypersensitivity, such as the Mantoux reaction, because of outstanding similarities between the two phenomena. It can be suggested that animals with CS respond also to intradermal injection of the conjugate of hapten and protein as well as to epicutaneous application of hapten. However, evidence against this has been reported. In the present experiments, delayed-type skin reaction (DSR) was successfully obtained in JY1 strain guinea pigs sensitized by painting the skin with 2,4-dinitrochlorobenzene using in vitro dinitrophenylated epidermal cell suspension (DNP-EC) as antigen for a delayed intradermal test. The experiment using anti-Ia alloantiserum and complement showed that the elicitation of DSR is due to the presence of Ia-positive cells (presumably Langerhans cells) among DNP-ECs. The delayed intradermal test with the conjugates such as haptenated ECs in the animals with CS is considered to be an experimentally useful way of analysing the antigen in the sensitivity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00417321
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  • 4
    Digitale Medien
    Digitale Medien
    Histological study on the fate of haptenated epidermal cells injected intradermally in guinea pigs (1987)
    Springer
    Archives of dermatological research 279 (1987), S. S111 
    Details
    ISSN: 1432-069X
    Schlagwort(e): 2,4-Dinitrochlorobenzene ; 2,4-Dinitrophenylated epidermal cells ; Contact sensitivity ; Spontaneous flare ; Delayed-type skin reaction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The percutaneous administration of in vitro haptenated epidermal cells (EC) has become established as a procedure to produce contact sensitivity (CS) in experimental animals for routine use. The cells have also been found to elicit a significant delayed-type skin reaction by intradermal test in the animals sensitized by painting the skin with the hapten. The fate of 2,4-dinitrophenylated (DNP) isogeneic epidermal cell suspensions (EC) injected intradermally was investigated histologically in intact or 2,4-dinitrochlorobenzene (DNCB)-sensitized strain 13 guinea pigs to study the role of the cells in CS. DNP-EC were found to proliferate actively in the dermis and formed EC nests with central keratinization and then elicited inflammatory reaction associated with necrosis of the epidermal structures 7 days after injection in the intact animals. DNP-EC injected intradermally into the animals which had received and reacted against DNCB underwent a suppression of EC proliferation. These findings are discussed in relation to the role of the haptenated EC in CS.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00585933
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