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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 192 (1978), S. 299-308 
    ISSN: 1432-0878
    Keywords: Basal lamina biosynthesis ; Telencephalic capillaries ; Chick embryos ; 3H-proline autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'élaboration de la lame basale des capillaires télencéphaliques a été étudiée en autoradiographie chez des embryons de Poulet âgés de 16 à 19 jours d'incubation. C'est au cours de cette période du développement que la basale des vaisseaux cérébraux est activement synthétisée. Les animaux furent sacrifiés 2,15, 40 et 180 min après le début de l'injection intraveineuse d'une solution de proline tritiée. L'identification des cellules participant à la synthèse de la basale est facilitée par le fait que l'hydroxylation de la proline en hydroxyproline y est réalisée. Nos observations nous permettent de suggérer une participation active des cellules endothéliales, des péricytes, et peut-être des cellules gliales, à l'élaboration de la lame basale. Les différentes étapes de cette synthèse ont lieu, par ordre chronologique, dans l'ergastoplasme, puis dans l'appareil de Golgi, avant que le produit élaboré ne soit libéré dans l'espace périvasculaire par exocytose.
    Notes: Summary The elaboration of the basal lamina of telencephalic capillaries has been studied by autoradiography in chick embryos from day 16 to 19 of incubation. The cerebral vascular basal lamina is actively synthesized during the period of these stages of development. The animals were sacrificed 2, 15, 40 and 180 min after intravenous injection of 3H-proline. The hydroxylation of proline to hydroxyproline in the “secretory” cells permits their identification. Our observations suggest that endothelial cells, pericytes and perhaps glial cells participate in this elaboration. The various stages of the synthesis are performed sequentially in the ergastoplasm and then in the Golgi apparatus before the secretory material is released by exocytosis.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0878
    Keywords: Epididymis ; Basement membrane ; Anionic sites ; Differentiation ; Androgen ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Anionic binding sites in the lamina densa of the basement membrane of the rat epididymal epithelium were demonstrated ultrastructurally with the use of cationized polyethyleneimine (PEI). Enzyme digestion with heparitinase removed the anionic sites, indicating that they consist largely of heparan sulfates. The anionic sites are present as early as the 16th day of gestation on the interstitial face of the lamina densa; later during gestation they are localized on both faces of the lamina densa without further modification after birth. The distribution of the anionic sites was identical all along the epididymal duct. After castration and ligation of efferent ducts or in the state of cryptorchidism the sites were more numerous and located inside the thicker portion of the lamina densa. These alterations were more prominent in the initial segment compared to the distal segments, suggesting a differential androgen dependence of the reactive sites and their patterns of distribution.
    Type of Medium: Electronic Resource
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