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  • 3H-proline autoradiography  (1)
  • Fetal and postnatal life  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Anatomy and embryology 160 (1980), S. 11-27 
    ISSN: 1432-0568
    Keywords: Sertoli cells ; Rat ; Fetal and postnatal life ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Sertoli cells have various functions: mechanical (creation of two compartments in the seminiferous tubules, migration of germinal cells), secretory (secretion of anti-Müllerian hormone, inhibin, androgen-binding-protein and estrogen) and phagocytic. We report an ultrastructural study of the rat Sertoli cell during maturation and consider possible correlations between the acquisition of certain morphological characteristics and certain functions. During fetal life, the Sertoli cell possesses differentiated zones of junction with the gonocytes and seems to have a role in the migration of the gonocytes towards the periphery of the seminiferous tubule. The Sertoli cell performs the phagocytosis of the gonocytes which degenerate during their migration, and seems to be the site of production of protein granules, whose presence can be related to the synthesis of anti-Müllerian hormone. After birth and before puberty, when the inclusions resembling secretory granules disappear, the Sertoli cell membranes in contact with spermatocytes II and spermatids differentiate, forming, through the differentiated junctional complexes, two compartments (adluminal and luminal) in the seminiferous tubules. Finally, they acquire the characteristics of active secretory cells, capable, in particular, of steroid synthesis.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 192 (1978), S. 299-308 
    ISSN: 1432-0878
    Keywords: Basal lamina biosynthesis ; Telencephalic capillaries ; Chick embryos ; 3H-proline autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'élaboration de la lame basale des capillaires télencéphaliques a été étudiée en autoradiographie chez des embryons de Poulet âgés de 16 à 19 jours d'incubation. C'est au cours de cette période du développement que la basale des vaisseaux cérébraux est activement synthétisée. Les animaux furent sacrifiés 2,15, 40 et 180 min après le début de l'injection intraveineuse d'une solution de proline tritiée. L'identification des cellules participant à la synthèse de la basale est facilitée par le fait que l'hydroxylation de la proline en hydroxyproline y est réalisée. Nos observations nous permettent de suggérer une participation active des cellules endothéliales, des péricytes, et peut-être des cellules gliales, à l'élaboration de la lame basale. Les différentes étapes de cette synthèse ont lieu, par ordre chronologique, dans l'ergastoplasme, puis dans l'appareil de Golgi, avant que le produit élaboré ne soit libéré dans l'espace périvasculaire par exocytose.
    Notes: Summary The elaboration of the basal lamina of telencephalic capillaries has been studied by autoradiography in chick embryos from day 16 to 19 of incubation. The cerebral vascular basal lamina is actively synthesized during the period of these stages of development. The animals were sacrificed 2, 15, 40 and 180 min after intravenous injection of 3H-proline. The hydroxylation of proline to hydroxyproline in the “secretory” cells permits their identification. Our observations suggest that endothelial cells, pericytes and perhaps glial cells participate in this elaboration. The various stages of the synthesis are performed sequentially in the ergastoplasm and then in the Golgi apparatus before the secretory material is released by exocytosis.
    Type of Medium: Electronic Resource
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