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  • Oryza sativa L.  (5)
  • AFLP markers  (2)
  • Restriction fragment length polymorphism (RFLP)  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Application of a rice field experimental error distribution function to nitrogen-phosphorus-potassium fertilizer response model analysis (1996)
    Springer
    Nutrient cycling in agroecosystems 47 (1996), S. 1-5 
    Details
    ISSN: 1573-0867
    Keywords: error distribution ; fertilizer experiment ; NPK response ; Oryza sativa L. ; outliers ; regression model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A distribution function of rice yield deviations from the mean was developed from field experiments with 555 plots at 16 sites in Zhejiang province, China, for three years. The deviation distribution in interval of 50kg/ha appeared as a symmetrical distribution with a high peak (Mean=0.279 [kg/ha], SD=240.686 [kg/ha]). Normality test using Kolmogrove-Smirnov test between the observed cumulative distribution and the normal cumulative distribution function indicates that the observed deviation distribution is not normal. An empirical exponential cumulative distribution function was developed. The distribution function was used to remove outliers during the development of a rice yield fertilizer response model, based on data from a non-replicated NPK field experiment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01985713
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  • 2
    Electronic Resource
    Electronic Resource
    Rice seedling tolerance to phosphorus stress in solution culture and soil (1998)
    Springer
    Nutrient cycling in agroecosystems 51 (1998), S. 95-99 
    Details
    ISSN: 1573-0867
    Keywords: Oryza sativa L. ; phosphorus stress tolerance ; screening parameter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Five rice genotypes with different performance under P-stress condition were tested in both a soil pot experiment and a solution culture experiment under different P supply conditions to evaluate rice performance to P stress. The relative parameters, defined as a ratio of the parameters measured under P stress condition to the parameters measured under P sufficient condition, were used to evaluate the genotypic variation in tolerance. It was found that relative tillering ability (RTA), relative root number (RRN) and relative root length (RRL), based on ratio of the measurements at 1 ppm P level to these at 10 ppm P level in solution culture after 2-week growth, could be used to assess the genotypic tolerance to P stress in terms of P uptake ability and biomass production under P stress condition. Relative P uptake ability (RPUP) and relative P use efficiency (RPUE) were relatively independent parameters with each other, and no parameters from solution culture were found to be significantly correlated with RPUE under soil condition. The result indicates that a ratio of tiller number measured at 1 ppm P to that measured at 10 ppm P after two weeks growth under solution culture condition may be used as a simple and reliable screening parameter for rice tolerance to P stress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009716330296
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    QTLs and epistasis for aluminum tolerance in rice (Oryza sativa L.) at different seedling stages (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 1295-1303 
    Details
    ISSN: 1432-2242
    Keywords: Key words Oryza sativa L. ; AFLP markers ; RFLP markers ; Aluminum tolerance ; QTLs ; Epistasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  To investigate the genetic background for aluminum (Al) tolerance in rice, a recombinant inbred (RI) population, derived from a cross between an Al-sensitive lowland indica rice variety IR1552 and an Al-tolerant upland japonica rice variety Azucena, was used in culture solution. A molecular linkage map, together with 104 amplified fragment length polymorphism (AFLP) markers and 103 restriction fragment length polymorphism (RFLP) markers, was constructed to map quantitative trait loci (QTLs) and epistatic loci for Al tolerance based on the segregation for relative root length (RRL) in the population. RRL was measured after stress for 2 and 4 weeks at a concentration of 1mM of Al3+ and a control with a pH 4.0, respectively. Two QTLs were detected at both the 2nd and the 4th weeks on chromosomes 1 and 12 from unconditional mapping, while the QTL on chromosome 1 was only detected at the 2nd stress week from conditional mapping. The effect of the QTL on chromosome 12 was increased with an increase of the stress period from 2 to 4 weeks. The QTL on chromosome 1 was expressed only at the earlier stress, but its contribution to tolerance was prolonged during growth. At least one different QTL was detected at the different stress periods. Mean comparisons between marker genotypic classes indicated that the positive alleles at the QTLs were from the Al-tolerant upland rice Azucena. An important heterozygous non-allelic interaction on Al tolerance was found. The results indicated that tolerance in the younger seedlings was predominantly controlled by an additive effect, while an epistatic effect was more important to the tolerance in older seedlings; additionally the detected QTLs may be multiple allelic loci for Al tolerance and phosphorus-uptake efficiency, or for Al and Fe2+ tolerance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051438
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  • 4
    Electronic Resource
    Electronic Resource
    Molecular-marker-facilitated investigation on the ability to stimulate N2 fixation in the rhizosphere by irrigated rice plants (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 1177-1183 
    Details
    ISSN: 1432-2242
    Keywords: Oryza sativa L. ; Atom 15N% excess ; N2 fixation ; Restriction fragment length polymorphism (RFLP)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An F2 population, consisting of 231 individuals derived from a cross between rice cultivars with a similar growing duration, Palawan and IR42, was utilized to investigate the genetic nature of rice varietal ability to stimulate N2 fixation in the rice rhizosphere. To assess rhizospheric N2 fixation, an isotope-enriched 15N dilution technique was employed, using 15N-stabilized soil in pots. IR42, an indica variety, had 23% higher N derived from fixation (Ndfa) than Palawan, a javanica genotype. Normal segregation of atom% 15N excess was obtained in the F2 population, with an average of 0.218 with 8% of plants below IR42 (0.188) and 10% of plants above Palawan (0.248). One-hundred-and-four RFLP markers mapped on 12 chromosomes were tested for linkage to the putative QTLs. Significant (P〈0.01) associations between markers and segregation of atom% 15N excess were observed for seven marker loci located on chromosomes 1, 3, 6 and 11. Four QTLs defined by the detected marker loci were identified by interval-mapping analysis. Additive gene action was found to be predominant, but for at least one locus, dominance and partial dominance effects were observed. Significant (P〈0.01) epistatic effects were also identified. Individual marker loci detected between 8 and 16% of the total phenotypic variation. All four putative QTLs showed recessive gene action, and no phenotypic effects associated with heterozygosity of marker loci were observed. The results of this study suggest that rice genetic factors can be identified which affect levels of atom% 15N excess in the soil by interacting with diazotrophs in the rice rhizosphere.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220926
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  • 5
    Electronic Resource
    Electronic Resource
    Non-allelic interaction conditioning spikelet sterility in an F2 population of indica/japonica cross in rice (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 825-829 
    Details
    ISSN: 1432-2242
    Keywords: Oryza sativa L ; Spikelet fertility ; Non-allelic interaction ; Restriction fragment length polymorphism (RFLP)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Significant segregation of spikelet fertility occurred in an F2 population derived from a spikelet fertility-normal F1 hybrid produced by a cross between ‘Palawan’, a japonica variety, and ‘IR42’, an indica variety. To identify factors controlling the fertility segregation, we used 104 RFLP markers covering all 12 rice chromosomes to investigate the association of spikelet fertility and marker segregation. We found that the segregation of two sets of gene pairs was significantly (P 〈 0.001) associated with fertility segregation. The first pair of genes was linked to RFLP marker RG778 on chromosome 12 and RFLP markers RG690/RG369 on chromosome 1. A significant reduction in fertility was observed when the plants were homozygote at RG778 with the indica allele as well as homozygote at RG690/RG369 with the japonica allele. The second pair of genes was linked to RG218 on chromosome 12 and RG650 on chromosome 7, respectively. The recombinant homozygote at these two loci showed a significant reduction on spikelet fertility. The non-allelic interaction effect was further modified by a gene linked to RG778, resulting in even lower fertility. The results of this study provides the first evidence of chromosomal localization of sporophytic sterility genes whose interaction can result in a reduction of spikelet fertility in the F2 derived from fertility-normal F1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223887
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Mapping QTLs for phosphorus deficiency tolerance in rice (Oryza sativa L.) (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1361-1369 
    Details
    ISSN: 1432-2242
    Keywords: Key words Oryza sativa L. ; AFLP markers ; RFLP markers ; Phosphorus deficiency tolerance ; QTL analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The amplified fragment length polymorphism (AFLP) technique combined with selective genotyping was used to map quantitative trait loci (QTLs) associated with tolerance for phosphorus (P) deficiency in rice. P deficiency tolerant cultivar IR20 was crossed to IR55178-3B-9-3 (sensitive to P-deficiency) and 285 recombinant inbred lines (RILs) were produced by single-seed descent. The RILs were phenotyped for the trait by growing them in P-sufficient (10.0 mg/l) and P-deficient (0.5 mg/l) nutrient solution and determining their relative tillering ability at 28 days after seeding, and relative shoot dry weight and relative root dry weight at 42 days after seeding. Forty two of each of the extreme RILs (sensitive and tolerant) and the parents were subjected to AFLP analysis. A map consisting of 217 AFLP markers was constructed. Its length was 1371.8 cM with an average interval size of 7.62 cM. To assign linkage groups to chromosomes, 30 AFLP and 26 RFLP markers distributed over the 12 chromosomes were employed as anchor markers. Based on the constructed map, a major QTL for P-deficiency tolerance, designated PHO, was located on chromosome 12 and confirmed by RFLP markers RG9 and RG241 on the same chromosome. Several minor QTLs were mapped on chromosomes 1, 6, and 9.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051030
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  • 7
    Electronic Resource
    Electronic Resource
    Molecular markers linked to genes underlying seedling tolerance for ferrous iron toxicity (1997)
    Springer
    Plant and soil 196 (1997), S. 317-320 
    Details
    ISSN: 1573-5036
    Keywords: ferrous iron toxicity ; molecular markers ; Oryza sativa L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A double haploid (DH) population consisting of 123 lines derived from a japonica variety, Azucena, and an indica variety, IR64, and 100 BC1F1 (Azucena) lines were cultivated hydroponically using two treatments: one with excess Fe2+ at the concentration of 250 mg L-1 and a control with standard nutrient solution. Genotypic tolerance was evaluated using an index scale based on degree of leaf bronzing and relative decease in shoot dry weight (RDSDW) Toxic symptoms were not observed for Azucena and BClFl plants. In contrast, index values for the DH population indicated segregation for tolerance, and IR64 was moderately sensitive. Molecular marker loci associated with variations in index values and in RDSDW, and gene loci for tolerance were detected using 175 Markers mapped on all 12 chromosomes by single marker loci and interval mapping. Two gene loci were identified to be flanked by RG345 and RG381, and linked to RG810, respectively, on chromosome 1 for both index values and RDSDW. They explained 32% and 13% of the total variation in the index values, and 15% and 21 % in the RDSDW in the population, respectively. The variation in RDSDW was also explained by a locus linked to RG978 on chromosome 8 by about 10%. Comparison of the two marker genotypic class means indicated that the tolerant alleles were from Azucena at the first locus on chromosome 1 and the locus on chromosome 8, and that at the second locus on chromosome 1 from IR64.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004288427140
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  • 8
    Electronic Resource
    Electronic Resource
    Genetic control of seedling tolerance to aluminum toxicity in rice (1997)
    Springer
    Euphytica 97 (1997), S. 289-293 
    Details
    ISSN: 1573-5060
    Keywords: Oryza sativa L. ; aluminum tolerance ; genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An uncomplete diallel analysis was conducted for 56 F1 progenies derived from 8 male × 7 female parents with differential Al tolerance based on root tolerance index (RTI) in a solution culture with Al concentration of 1 mM Al. Remarkable variation in RTI among the parents was observed after 6 weeks in culture. Significant (P 〈 0.001) general combine ability (GCA) variance for both male and female parents and specific combine ability (SCA) variance were observed. The variance of GCA was much higher than that of SCA, indicating greater additive expression of the tolerant trait. Higher narrow-sense heritability (48%) was detected, indicating the possibility of a genetic gain in selection for Al tolerance based on RTI. The tolerant performance of F1 progenies appeared to be influenced by the susceptible genotype. suggesting the inconsistent dominance effect. The possible mechanisms of the apparent inconsistent dominance was discussed in terms of the genetic background in wheat. Two restore lines, Pedel and 02428, and one sterile variety, XieqinzaoA, were found to be high in the GCA effect and SCA variance in this case. These genotypes may be useful in development of hybrid rice production on acid soils.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003084409038
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