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  • DNA gyrase  (2)
  • APC  (1)
  • Dictyostelium  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The budding yeast cohesin gene SCC1/MCD1/RHC21 genetically interacts with PKA, CDK and APC (1999)
    Springer
    Current genetics 36 (1999), S. 329-338 
    Details
    ISSN: 1432-0983
    Keywords: Key words Cohesin ; SCC1/MCD1/RHC21 ; Multicopy suppressor ; PKA ; CDC28 ; CDC20 ; PDS1 ; APC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cohesin is a protein that plays a key role in the cohesion and separation of sister chromatids. During the duplication of chromatids, cohesin holds sister chromatids together until the onset of anaphase, and thereby prevents the premature separation of sister chromatids which would otherwise jeopardize the faithful segregation of chromosomes. To investigate the molecular mechanisms of sister chromatid cohesion, we have isolated multicopy suppressors of a temperature-sensitive (ts) mutation in the SCC1/MCD1/RHC21 gene which encodes a component of the cohesin complex in budding yeast. Isolation of multicopy suppressors of rhc21-sk16 and further genetic analyses revealed that several distinct biological pathways are involved in the regulation of SCC1/MCD1/RHC21 function. Firstly, PDE2 and BCY1, each of which inhibits the activity of protein kinase A (PKA), suppressed the temperature sensitivity of the rhc21-sk16 mutant . Secondly, PDE2 suppressed the temperature sensitivity of the cdc16-1 mutant. These results suggest that SCC1/MCD1/RHC21 is negatively regulated by the PKA pathway via the anaphase promoting complex (APC). Thirdly, ZDS1, a multicopy suppressor of cdc28-1N, and its homologue ZDS2 were isolated as multicopy suppressors of rhc21-sk16. Furthermore, the rhc21-sk16 mutant did not grow in the presence of the cdc28-1N mutation. Hence, SCC1/MCD1/RHC21 is positively regulated by the mitotic CDK, CDC28. Finally, SCC1/MCD1/RHC21 was found to interact genetically with CDC20, an activator of APC. Overexpression of CDC20 suppressed the temperature sensitivity of rhc21-sk16, and rhc21-sk16 was shown to be synthetically lethal with cdc20-1. In addition, the growth of the rhc21-sk16 mutant was inhibited by overproduction of the anaphase inhibitor Pds1p, whose degradation is mediated by Cdc20p in APC-dependent proteolysis. The functional relationships between SCC1/MCD1/RHC21 and PKA, CDK or APC are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050507
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Formation of deletion in Escherichia coli between direct repeats located in the long inverted repeats of a cellular slime mold plasmid: Participation of DNA gyrase (1988)
    Springer
    Molecular genetics and genomics 214 (1988), S. 1-5 
    Details
    ISSN: 1617-4623
    Keywords: Escherichia coli ; Dictyostelium ; DNA gyrase ; Deletion ; Plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We constructed a recombinant plasmid containing the 2.1 kb HindIII fragment of plasmid pDG1, isolated from the cellular slime mold (Dictyostelium sp. strain GA11), and using pAG60 as cloning vector. We found that deletions of the recombinant plasmid took place frequently in Escherichia coli wild-type cells. However, the deletion was not observed when the plasmid was introduced into a strain that was an isogenic temperature-sensitive mutant of the gyrA gene. These results suggest that E. coli DNA gyrase is involved in the mechanisms of the deletion formation. It was shown that the 1.0 kb deletant derived from the 2.1 kb HindIII insert was produced by elimination of a 1.1 kb region. Sequence analysis of the deletants showed that cutting and rejoining took place between two out of the six nearly perfect direct repeats [21 bp palindromic sequences; AAAAAA(T/C)GGC(G/C)GCC(A/G)TTTTTT], located near the distal ends of the inverted repeats, preserving one copy of the repeats. These sequences consist of local short inverted repeats, where cutting and rejoining occur at one of the two regions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00340170
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Molecular analysis of the recombination junctions of λ bio transducing phases (1991)
    Springer
    Molecular genetics and genomics 230 (1991), S. 60-64 
    Details
    ISSN: 1617-4623
    Keywords: Specialized transduction ; bio gene ; Illegitimate recombination ; REP sequence ; DNA gyrase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To examine the mechanism of recombination involved in the formation of specialized transducing phage during the induction of bacteriophage λ we have determined the nucleotide sequences of the recombination junctions of λbio phages. The results indicate that abnormal excision takes place at many sites on both bacterial and phage genomes and that the recombination sites have short regions of homology (5–14 bp). Some of the sequences of the recombination sites were similar to the consensus sequences of DNA gyrase-cleavage sites and repetitive extragenic palindromic (REP) sequences. These results showed that abnormal excision is a type of illegitimate recombination. The possible involvement of DNA gyrase in this recombination is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290651
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    Paper (German National Licenses)
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