ISSN:
1432-1912
Keywords:
C-1300 neuroblastoma cells
;
6-Aminonicotinamide
;
Differentiation
;
Acetylcholinesterase activity
;
Growth inhibition
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Addition of 6-AN (0.01 mg/ml) to growing cultures of C-1300 neuroblastoma cells strongly reduced cell division. This growth inhibition was accompanied by a higher cell volume and a lower protein content per cell as compared to controls. Concurrently the specific activity of AChE increased markedly in controls and 6-AN-treated cultures. During the experimental periods the specific activity of AChE was significantly higher after 6-AN. Morphologically, 6-AN-treated cultures showed characteristic signs of differentiation, i.e. enlarged, flattened cells with long branched processes. The described effect of 6-AN on growth and differentiation of neuroblastoma cells was less pronounced if cells received the antimetabolite after a subcultivation period of 5 days.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00508280
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