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  • Acid phosphatase  (1)
  • Morphological development  (1)
  • Protoplasts regeneration  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 105 (1975), S. 277-282 
    ISSN: 1432-072X
    Keywords: Trichoderma viride ; Mycelial walls ; Regeneration walls ; Aberrant tubes ; β-(1–3)Glucan ; β-(1–6)Glucan ; Chitin ; Fungal protoplasts ; Protoplasts regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When incubated in Winge medium, protoplasts from Trichoderma viride obtained by treatment with Micromonospora chalcea or Streptomyces venezuelae RA lytic systems first synthesized an aberrant wall, different from the normal one; it was aseptate, larger and irregular in size and length. They then regenerated a new wall, similar to the original one from which they were liberated. Analysis showed that the wall polymers were mainly β-(1–3) glucan, β-(1–6) glucan and chitin in the normal walls, whereas chitin was absent in aberrant tubes. These results are discussed below, together with electron micrographs of aberrant and normal walls.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-072X
    Keywords: β-Glucanase ; Invertase ; Acid phosphatase ; 2-deoxy-d-glucose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The synthesis of β-glucanase either by cells or by protoplasts of the yeast Pichia polymorpha has been found to occur in the presence of 2-deoxy-d-glucose in the growth medium. On the other hand, the synthesis of typical extracellular proteins such as invertase and acid phosphatase is strongly affected by the presence of the drug. The degree of inhibition is, however, directly related to the 2-deoxy-d-glucose concentration.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-072X
    Keywords: Trichoderma viride ; Polyoxin D ; Mycelial walls ; Aberrant tubes ; Cell wall synthesis ; Spore germination ; Morphological development ; Regenerating protoplasts ; Chitin ; β-(1-3) Glucan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When polyoxin D is added to a spore suspension of Trichoderma viride at a concentration from 50–100 μg/ml, it inhibits from 40–60% of germination. This percentage increases if dimethylsulfoxide (DMSO) is added. Mycelium growing in the presence of polyoxin D becomes irregular and loses its rigidity, showing several bulges along the hypha. Under the electron microscope the features of the cell wall and cytoplasmic content are apparently normal. Nevertheless, after incubation with different lytic systems or with (14C)glucose, it can be seen that polyoxin D partially inhibits the biosynthesis of β-(1-3)glucan and the biosynthesis of chitin to a greater extent attaining inhibition of 83% at 100 μg/ml of the antibiotic concentration. Regenerating protoplasts are less affected by polyoxin D. They do regenerate slower but the percentage of regeneration is more than 80%. Aberrant tubes synthesized by these protoplasts are not affected, they manifest their usual morphology and lack of chitin is confirmed in their composition.
    Type of Medium: Electronic Resource
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