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  • Apoptosis  (4)
  • Admittance plethysmography  (3)
  • plasma diagnostics  (3)
  • Adenosine  (2)
  • Autolysis  (2)
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Materialart
Erscheinungszeitraum
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  • 1
    Digitale Medien
    Digitale Medien
    Distinct kinetics of subunit autolysis in mammalian m-calpain activation
    Amsterdam : Elsevier
    FEBS Letters 346 (1994), S. 263-267 
    Details
    ISSN: 0014-5793
    Schlagwort(e): Autolysis ; Calcium ; Proteolysis ; m-Calpain
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Physik
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0014-5793(94)00487-0
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  • 2
    Digitale Medien
    Digitale Medien
    Distinct kinetics of subunit autolysis in mammalian m-calpain activation
    Amsterdam : Elsevier
    FEBS Letters 346 (1994), S. 263-267 
    Details
    ISSN: 0014-5793
    Schlagwort(e): Autolysis ; Calcium ; Proteolysis ; m-Calpain
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Physik
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(94)00487-0
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  • 3
    Digitale Medien
    Digitale Medien
    Acetylcholine and adenosine activate the G protein-gated muscarinic K+channel in ferret ventricular myocytes (1995)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 351 (1995), S. 610-617 
    Details
    ISSN: 1432-1912
    Schlagwort(e): Key words Ferret ; Ventricular myocytes ; Acetylcholine ; Adenosine ; Muscarinic K+ channel ; G protein
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract  The properties of the K+ channel activated by acetylcholine (ACh) and adenosine (Ado) were examined in single ferret ventricular myocytes using patch-clamp techniques. In the whole-cell configuration, ACh and Ado induced an inwardly rectifying K+ current and shortened the action potential duration. The effect of ACh was blocked by atropine, while the Ado effect was interrupted by 8-cyclopentyl,1,2-dipropyl xanthine, a specific Ado A1 receptor antagonist. In cell-attached recordings, ACh and Ado added to the pipette solution activated a single population of inwardly rectifying K+ channels, distinct from the i K1 channel. The channel had a slope conductance of ∼40 pS in symmetrical 150 mM K+ solutions and a mean open time of 0.8 ms. Excision of the patch into the inside-out patch configuration in guanosine triphosphate (GTP)-free solution abolished the channel activity. The channel was reversibly reactivated by adding GTP to the intracellular side of the patch. GTPγS activated the channel irreversibly. When the inside-out patch was treated with the A protomer of pertussis toxin (PTX), intracellular GTP no longer activated the K+ channel. The results show that ferret ventricular myocytes possess a K+ channel activated by both muscarinic and Ado A1 receptors. Its electrophysiological properties and the gating by a PTX-sensitive G protein in a membrane-delimited fashion are identical with those of the muscarinic K+ channels in nodal and atrial tissues of other species. In conclusion, the G protein-gated muscarinic K+ channel is expressed in ferret ventricular myocardium and may underlie the direct negative inotropism of ACh and Ado in this tissue.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00170160
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  • 4
    Digitale Medien
    Digitale Medien
    Acetylcholine and adenosine activate the G protein-gated muscarinic K+ channel in ferret ventricular myocytes (1995)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 351 (1995), S. 610-617 
    Details
    ISSN: 1432-1912
    Schlagwort(e): Ferret ; Ventricular myocytes ; Acetylcholine ; Adenosine ; Muscarinic K+ ; channel ; G protein
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The properties of the K+ channel activated by acetylcholine (ACh) and adenosine (Ado) were examined in single ferret ventricular myocytes using patch-clamp techniques. In the whole-cell configuration, ACh and Ado induced an inwardly rectifying K+ current and shortened the action potential duration. The effect of ACh was blocked by atropine, while the Ado effect was interrupted by 8-cyclopenty1,1,2-dipropyl xanthine, a specific Ado A1 receptor antagonist. In cell-attached recordings, ACh and Ado added to the pipette solution activated a single population of inwardly rectifying K+ channels, distinct from the i K1 channel. The channel had a slope conductance of ∼ 40 pS in symmetrical 150 mM K+ solutions and a mean open time of 0.8 ms. Excision of the patch into the inside-out patch configuration in guanosine triphosphate (GTP)-free solution abolished the channel activity. The channel was reversibly reactivated by adding GTP to the intracellular side of the patch. GTPγS activated the channel irreversibly. When the inside-out patch was treated with the A protomer of pertussis toxin (PTX), intracellular GTP no longer activated the K+ channel. The results show that ferret ventricular myocytes possess a K+ channel activated by both muscarinic and Ado A1 receptors. Its electrophysiological properties and the gating by a PTX-sensitive G protein in a membrane-delimited fashion are identical with those of the muscarinic K+ channels in nodal and atrial tissues of other species. In conclusion, the G protein-gated muscarinic K+ channel is expressed in ferret ventricular myocardium and may underlie the direct negative inotropism of ACh and Ado in this tissue.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00170160
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  • 5
    Digitale Medien
    Digitale Medien
    Apoptosis in human gastric mucosa, chronic gastritis, dysplasia and carcinoma: analysis by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (1996)
    Springer
    Virchows Archiv 428 (1996), S. 229-235 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Apoptosis ; TUNEL ; Human gastric mucosa ; Carcinoma ; Ki-67
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We examined the existence and distribution of apoptotic cells in human gastric mucosa, chronic gastritis, adenomatous dysplasias and carcinomas in 15 surgically removed stomachs in which dysplasia and carcinoma were found simultaneously. Serial sections were cut for immunohistochemistry for p53 oncoprotein and Ki-67 antigen, and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labelling (TUNEL). TUNEL signal-positive apoptotic cells were rare in normal mucosa, while a few apoptotic cells were noted in gastritic mucosa and intestinal metaplasia, intermingled with Ki-67 antigen-positive cells forming a generative cell zone. This suggests the cell-cycle-dependent apoptosis of gastric mucosa. The frequency of apoptotic cells per crypt was higher in incomplete than in complete metaplasia, implying greater underlying DNA damage in the former. TUNEL indices (TI; percentage of TUNEL-positive cells in tumour cells) were slightly higher in adenomatous dysplasias (4.9±2.1) than in carcinoma (3.9±1.1), but there was no no statistical difference. Ki-67 indices (KI; percentage of Ki-67 antigen-positive cells in tumour cells) were significantly (P〈0.05) higher in carcinomas than in dysplasias. Thus, gastric adenomatous dysplasias were characterized by relatively higher TI and lower KI, which might reflect a more static growth potential. The expression of p53 oncoprotein in cancer cells is thought to be an apoptosis-suppressing event, although its precise role remains to be elucidated. Overall, these results indicate that apoptosis plays a crucial part in the morphogenesis of gastritic mucosa including intestinal metaplasia, and that the process is correlated both with tumourigenesis and with proliferative activity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00196695
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  • 6
    Digitale Medien
    Digitale Medien
    Role of the bcl-2/bax pathway in hepatocyte apoptosis during acute rejection after rat liver transplantation (1998)
    Springer
    Transplant international 11 (1998), S. S179 
    Details
    ISSN: 1432-2277
    Schlagwort(e): Key words Liver transplantation ; Apoptosis ; TUNEL method ; bax ; bcl-2
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract It is well established that hepatocytes undergo apoptotic cell death in the course of rejection of liver grafts. The present study was designed to investigate the role of the bcl-2/bax pathway in liver allograft tissue. Orthotopic liver transplantation was performed in three groups of rats: group 1, a syngeneic combination (Lewis to Lewis), group 2, an allogeneic combination (ACI to Lewis), and group 3, an allogeneic combination (ACI to Lewis) treated with 15-deoxyspergualin. The number of apoptotic cells identified by the TUNEL method in the grafted liver reflected the severity of acute rejection. In group 1, both bcl-2 mRNA and bax mRNA were expressed in trace amounts. In group 2, bcl-2 mRNA was slightly expressed while the expression of bax mRNA rose steadily. In group 3, bcl-2 mRNA expression levels remained similar to group 1, while bax expression levels exceeded those in group 1, but were less than in group 2. Expression of bcl-2 mRNA was stationary in comparison with expression of bax mRNA. Significantly higher levels of bax mRNA were expressed from day 4 in group 2 than in group 1 (on postoperative days 4, 6, and 8, P 〈 0.05, group 2 vs group 1). We also investigated bax protein and results consistent with the mRNA analysis data were obtained. These findings suggest that apoptotic cell death in liver allograft rejection is regulated, at least in part, by bax.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001470050456
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  • 7
    Digitale Medien
    Digitale Medien
    Expression of bcl-2 homologue mRNAs in rat liver allograft: rejection-induced cell apoptosis is associated with upregulation of bax and bcl-xs expression (1998)
    Springer
    Transplant international 11 (1998), S. S284 
    Details
    ISSN: 1432-2277
    Schlagwort(e): Key words Liver transplantation ; Apoptosis ; bcl-2 ; bax ; bcl-x
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Apoptosis is considered to play an important role in rejection of organ transplants, although the precise mechanism has not been elucidated. In this study, we screened for the expression of bcl-2 homologues (bcl-2, bax, bcl-xl, and bcl-xs) and Fas ligand (FasL) by RT–PCR method in grafts during acute rejection in rats following liver transplantation. Both bax and bcl-xs (inducers of apoptosis) mRNA levels increased steadily in the allografted group from postoperative day (POD) 2 to 8, while no remarkable changes of bcl-2 and bcl-xl expression (inhibitors of apoptosis) were recognized. Significant induction of FasL gene expression was observed in the allografted group on POD 4 and expression gradually decreased thereafter, although minimal FasL mRNA expression was seen in isografts. Our results indicated, for the first time, that rejection-induced cell apoptosis is closely associated with upregulation of bax and bcl-xs expression besides FasL, but not with down-regulation of bcl-xl.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001470050480
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  • 8
    Digitale Medien
    Digitale Medien
    Anti-Fas antibody-induced apoptosis in human colorectal carcinoma cell lines: role of the p53 gene (1998)
    Springer
    Apoptosis 3 (1998), S. 431-437 
    Details
    ISSN: 1573-675X
    Schlagwort(e): Apoptosis ; Colorectal Carcinoma ; FAS ; p53
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The cell surface Fas antigen transducts an apoptotic signal by its crosslinking with Fas ligand or anti-Fas antibody in a variety of human cultured cells. In this study, we examined the expression of Fas antigen and its mediation of apoptosis in six human colorectal carcinoma cell lines. A flow cytometric analysis revealed that LoVo, DLD-1, WiDr and SW837 cell lines showed higher expression levels of Fas antigen, in contrast to lower expression in COLO201 and COLO320DM. Interferon-γ enhanced the expression of Fas antigen in all of the cell lines examined. Both Fas ligand and Fas-associated phosphatase-1 (FAP-1) were expressed only in COLO320DM. Anti-Fas antibody induced apoptosis in LoVo carrying wild-type p53 gene, but not in the other five cell lines carrying mutated p53 gene. The transfection of wild-type p53 gene using an adenovirous vector upregulated P53 protein in WiDr and SW837 cells, both of which showed, however, no increase in apoptotic cells by anti-Fas antibody treatment. These results indicated that (1) Fas antigen was variably expressed, regardless of the p53 gene status and (2) the susceptibility to anti-Fas antibody-mediated apoptosis did not correlate to Fas, Fas ligand or FAP-1 expression levels. Therefore, we conclude that wild-type P53 expression might not necessarily be essential for Fas-mediated apoptosis in human colorectal carcinoma cell lines.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009662619907
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  • 9
    Digitale Medien
    Digitale Medien
    A 28 μm water-vapor laser interferometer for plasma diagnostics (1983)
    Springer
    International journal of infrared and millimeter waves 4 (1983), S. 549-559 
    Details
    ISSN: 1572-9559
    Schlagwort(e): interferometer ; polarimeter ; water-vapor laser ; far-infrared ; plasma diagnostics ; compact toroid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract A high-power CW 27.972 μm water-vapor laser has been developed for interferometric and polarimetric measurements of plasmas. The maximum output power and the power density in the cavity of the laser with 2.0 cm bore and 220 cm length reach 85 mW and 109 μW/cm3, respectively. An interferometer using this laser is constructed and its performance is examined as a study on polarimetric method of magnetic field measurement. Preliminary interferometric experiments are also carried out on a compact toroidal plasma with field-reversal configuration.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01009394
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  • 10
    Digitale Medien
    Digitale Medien
    A CW 28 μm water vapor laser for plasma diagnostics (1981)
    Springer
    International journal of infrared and millimeter waves 2 (1981), S. 1199-1208 
    Details
    ISSN: 1572-9559
    Schlagwort(e): water vapor laser ; plasma diagnostics ; interferometer ; waveguide laser ; far-infrared
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract A high-power CW 27.972 μm water vapor laser has been constructed in order to use as a light source of an interferometer for plasma diagnostics. The laser consists of 220 cm length and 2.0 cm small bore Pyrex glass discharge tube, semi-confocal resonator and Michelson coupler. From the measurement of cavity detuning curves, it is found that two waveguide modes, EH11 and EH21 oscillate. The increase of the output power is found to become more than seven times larger by adding He(50%)−H2(50%) mixed gas. Under the optimum condition, the maximum output power of 65 mW or the output power per unit cavity volume of 83 μW/cm3 is obtained.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01007084
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