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  • Immunohistochemistry  (11)
  • Astrocyte  (4)
  • Adenylate cyclase  (3)
  • Amyotrophic lateral sclerosis  (3)
  • 1
    Digitale Medien
    Digitale Medien
    Desensitization of the epidermal adenylate cyclase system: agonists and phorbol esters desensitize by independent mechanisms
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Molecular Cell Research 1093 (1991), S. 95-101 
    Details
    ISSN: 0167-4889
    Schlagwort(e): (Pig epidermis) ; Adenylate cyclase ; Agonist ; Desensitization ; Phorbol ester
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Medizin , Physik
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0167-4889(91)90143-L
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  • 2
    Digitale Medien
    Digitale Medien
    Immunohistochemical reaction patterns of keratins in MNNG-induced shrew oesophageal carcinomas (1994)
    Springer
    Virchows Archiv 424 (1994), S. 267-271 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Keratin ; Immunohistochemistry ; MNNG ; Oesophageal carcinoma ; Shrew
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The distribution of keratins in N-methyl-N'-nitro-N-nitrosoguanidine-induced oesophageal carcinomas in shrews was tested immunohistochemically, using a panel of seven different monoclonal antibodies. The studies were done on methacarn-fixed paraffin-embedded tissue, using the labelled streptavidin biotin method, and the relationship between morphological characteristics and keratin reaction patterns in carcinomas was analysed and compared with that in adjacent “normal” oesophageal epithelium. In the normal oesophageal epithelia, KL1, AE1, AE3, CK8.12 and CK4.62 stained suprabasal cells, 312C8-1 reacted to basal cells, and KS-1A3 labelled all epithelial cells. In squamous cell carcinomas, almost all the cancer cells were labelled strongly by 312C8-1 and weakly by KS-1A3, while a few cells in the centres of the keratinized foci were stained by KL1, AE1, AE3, CK8.12, and CK4.62. Like human oesophageal carcinomas, shrew oesophageal carcinomas maintain expression of human keratin 14, as determined by 312C8-1. The expression of human keratin 13, as determined by KS-1A3, was down-regulated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00194610
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  • 3
    Digitale Medien
    Digitale Medien
    Parkinson's disease: an immunohistochemical study of Lewy body-containing neurons in the enteric nervous system (1990)
    Springer
    Acta neuropathologica 79 (1990), S. 581-583 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Parkinson's disease ; Lewy body ; Enteric nervous system ; Immunohistochemistry ; Vasoactive intestinal polypeptide
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary We performed immunohistochemical analysis of specimens from three autopsied patients with Parkinson's disease, using antibodies to tyrosine hydroxylase (TH), vasoactive intestinal polypeptide (VIP), somatostatin, met-enkephalin, leu-enkephalin and substance P in an attempt to reveal the types of neurons that contain Lewy bodies (LBs) in the paravertebral and celiac sympathetic ganglia and in the enteric nervous system of the alimentary tract. In the sympathetic ganglia, almost all LB-containing neuronal cell bodies and processes were immunoreactive for TH. In the alimentary tract, however, most LBs were found in the VIP-immunoreactive (VIP-IR) neuronal cell bodies and processes. In spite of the significant presence of TH-IR neuronal cell bodies and processes in the alimentary tract, LB-containing TH-IR neuronal elements were rarely encountered. These findings indicate that in the alimentary tract, the VIP neuron system is mainly involved in the disease process of Parkinson's disease.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00294234
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  • 4
    Digitale Medien
    Digitale Medien
    Hereditary dentatorubral-pallidoluysian atrophy: ubiquitinated filamentous inclusions in the cerebellar dentate nucleus neurons (1998)
    Springer
    Acta neuropathologica 95 (1998), S. 479-482 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Key words Dentatorubral-pallidoluysian atrophy ; Cerebellar dentate nucleus neuron ; Skein-like inclusion ; Ubiquitin ; Amyotrophic lateral sclerosis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We examined the cerebellar dentate nucleus (CDN) in 16 patients with hereditary dentatorubral-pallidoluysian atrophy (DRPLA), one of the neurodegenerative diseases caused by expansion of a CAG repeat encoding a polyglutamine tract in the disease protein. In all patients, some CDN neurons were found to contain ubiquitinated filamentous inclusions in their cytoplasm. On hematoxylin and eosin preparations, these filamentous inclusions were eosinophilic, basophilic or amphophilic, and were often found in areas of pale cytoplasm. Electron microscopy revealed that they consisted of bundles of filaments that were somewhat thicker than neurofilaments. These features of the present inclusions were indistinguishable from those of skein-like inclusions (SLI) previously described in the lower motor neurons in sporadic amyotrophic lateral sclerosis. We conclude that SLI can also occur in the CDN in DRPLA and believe that they reflect a characteristic pathological process in this disease.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004010050828
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  • 5
    Digitale Medien
    Digitale Medien
    NACP/α-synuclein-positive filamentous inclusions in astrocytes and oligodendrocytes of Parkinson’s disease brains (2000)
    Springer
    Acta neuropathologica 99 (2000), S. 14-20 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Key wordsα-Synuclein ; Astrocyte ; Oligodendrocyte ; Glial inclusion ; Parkinson’s disease
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The precursor of the non-Aβ component of Alzheimer’s disease amyloid (NACP), also called α-synuclein, is a major component of Lewy bodies in Parkinson’s disease (PD) as well as of neuronal and oligodendroglial cytoplasmic inclusions in multiple system atrophy. We previously reported argyrophilic, tau-negative glial inclusions in the midbrains of patients with PD and have now conducted immunocytochemical and ultrastructural examinations. The PD glial inclusions also are immunoreactive for NACP/α-synuclein, but not for β-synuclein, and ultrastructurally are composed of filamentous structures about 25–40 nm in diameter. Double immunolabeling showed that the inclusions were present in both astrocytic and oligodendroglial cells. They were located within the substantia nigra in 13 of 30 patients with PD and outside the nigra in 24. The number of inclusions was correlated with the severity of nigral neuronal loss. These findings indicate that abnormal accumulation of NACP/α-synuclein in glial cells is a pathological feature of PD related to its progression.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/PL00007400
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  • 6
    Digitale Medien
    Digitale Medien
    Ganglioglioma with a tanycytic ependymoma as the glial component (2000)
    Springer
    Acta neuropathologica 99 (2000), S. 310-316 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Key words Ganglioglioma ; Ependymoma (tanycytic variant) ; Neurofibrillary tangle ; Immunohistochemistry ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We studied a cystic ganglioglioma (GG) located in the right frontal lobe of the brain. Interestingly, the fibrillary spindle glial cells were often arranged in a fascicular pattern, and the generally uniform, round-to-oval delicate nuclei appeared to resemble those of ependymoma; and the neoplastic neurons often contained neurofibrillary tangles (NFTs). The glial component was positive for glial fibrillary acidic protein and occasionally contained granular or microvesicular structures positive for epithelial membrane antigen. Ultrastructural investigation revealed that the glial cells were ependymal in nature; intracytoplasmic lumina and intercellular microrosettes lined with cilia and microvilli, as well as long zonulae adherentes, were evident. In addition, chromogranin A-positive granular staining, neurosecretory-granule-like structures, and parallel arrays of microtubules were sometimes associated with the blood vessels. We considered the present case to be an unusual example of GG with an ependymoma, more precisely a tanycytic ependymoma, as the glial component; to our knowledge, the existence of ependymoma as the main glial component of this particular tumor has not been described before. The occurrence of NFTs, which has been reported in several cases of GG, was an additional, unusual feature.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/PL00007443
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  • 7
    Digitale Medien
    Digitale Medien
    Esthesioneuroblastoma: a nasal catecholamine-producing tumor of neural crest origin (1988)
    Springer
    Acta neuropathologica 76 (1988), S. 522-527 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Esthesioneuroblastoma ; Tyrosine hydroxylase (TH) ; Immunohistochemistry ; Ultrastructure ; Catecholamine-producing tumor
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary An esthesioneuroblastoma in a 16-year-old male was studied ultrastructurally and immunohistochemically, using antiserum against tyrosine hydroxylase (TH), a rate-limiting enzyme in the catecholamine-synthesizing pathway. Tumor cells were fairly uniform in appearance, showing scantly cosinophilic cytoplasm and round to oval hyperchromatic nuclei, and were arranged in nests and cords of various sizes. Ultrastructurally, individual tumor cells had well-developed cell organelles including polyribosomes, microtubules, intermediate filaments, centrioles, Golgi apparatus and mitochondria. Secretory-like granules were occasionally found, predominantly in the cell processes. Immunohistochemically, many tumor cells were shown to be immunoreactive for TH. This finding strongly suggested that the present tumor was capable of producing catecholamines and that it might be derived from certain sympathetic neuronal cell nests in the superior nasal cavity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00686393
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  • 8
    Digitale Medien
    Digitale Medien
    Paragangliomas of the craniocervical region (1987)
    Springer
    Acta neuropathologica 73 (1987), S. 227-232 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Paraganlioma ; Tyrosine hydroxylase(TH) ; Immunohistochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary An immunohistochemical study on tyrosine hydroxylase (TH), a rate-limiting enzyme in the catecholamine synthesizing pathway, was made on three cranioncervical region paragangliomas, two of which showed metastases to the cervical lymph nodes. In all of the original tumors, the majority of tumor cells showed positive immunostaining for TH of variable intensity in their cytoplasm regardless of their cytological features such as cellular and nuclear pleomorphism. The finding suggests that most tumor cells are capable of production of catecholamines and are derived from chief cells in the normal paraganglia. In cervical lymph nodes, however, no positive immunostaining for TH was observed in metastatic tumor cells, in contrast with the findings in the original tumors. The absence of TH immunoreactivity in metastatic tumor cells appears to be noteworthy in considering their malignant potential. Application of the TH immunohistochemistry to further cases appears important for the better understanding of this neoplasm, a catecholamine-producing tumor.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00686615
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  • 9
    Digitale Medien
    Digitale Medien
    Hereditary dentatorubral-pallidoluysian atrophy: detection of widespread ubiquitinated neuronal and glial intranuclear inclusions in the brain (1998)
    Springer
    Acta neuropathologica 96 (1998), S. 547-552 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Key words Dentatorubral-pallidoluysian atrophy ; Nuclear inclusion ; Ubiquitin ; Immunohistochemistry ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We examined the brains and spinal cords of seven patients with clinicopathologically and genetically confirmed hereditary dentatorubral-pallidoluysian atrophy (DRPLA) using an antibody against ubiquitin, and found small, round immunoreactive intranuclear inclusions in both neurons and glial cells in various brain regions. Ubiquitinated neuronal intranuclear inclusions (uNIIs) were consistently found in the striatum, the pontine nuclei, the inferior olivary complex, the cerebellar cortex and the dentate nucleus. Ubiquitinated glial intranuclear inclusions (uGIIs) were found less frequently than uNIIs. Most of the inclusion-bearing nuclei were of an astrocytic nature. Immunostaining with an antibody against DRPLA protein revealed similar immunoreactive neuronal and glial intranuclear inclusions, but in much smaller in numbers compared with uNIIs and uGIIs. Electron microscopy showed that such inclusions were composed of granular and filamentous structures. These findings strongly suggest that, in DRPLA, the occurrence of uNIIs and uGIIs is directly related to the causative gene abnormality (an expanded CAG repeat encoding polyglutamine), that neurons are affected much more widely than previously recognized and that glial cells are also involved in the disease process.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004010050933
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  • 10
    Digitale Medien
    Digitale Medien
    Ubiquitinated filamentous inclusions in cerebellar dentate nucleus neurons in dentatorubral-pallidoluysian atrophy contain expanded polyglutamine stretches (2000)
    Springer
    Acta neuropathologica 99 (2000), S. 615-618 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Key words Dentatorubral-pallidoluysian atrophy ; Cerebellar dentate nucleus neuron ; Skein-like inclusion ; Polyglutamine ; Immunohistochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We have recently reported that, in addition to the widespread occurrence of ubiquitinated neuronal intranuclear inclusions (NIIs), the restricted occurrence of ubiquitinated intracytoplasmic filamentous inclusions in the neurons of the cerebellar dentate nucleus (CDN) is a characteristic feature of dentatorubral-pallidoluysian atrophy (DRPLA). Interestingly, these neuronal intracytoplasmic filamentous inclusions (NIFIs) were morphologically indistinguishable from the skein-like inclusions (SLIs) described previously in the spinal anterior horn cells in amyotrophic lateral sclerosis (ALS). In the present study, we examined immunohistochemically the CDN in ten patients with clinicopathologically and genetically confirmed DRPLA and the spinal anterior horns in five patients with sporadic ALS, using a monoclonal antibody (1C2) directed against long polyglutamine stretches. In all of the patients with DRPLA, both the NIFIs and the NIIs were visualized clearly with 1C2. Conversely, in the patients with ALS all structures, including the SLIs, were completely negative. These findings indicate that in DRPLA, the NIFIs in the CDN are an alteration that is directly related to the causative gene abnormality (an expanded CAG repeat encoding polyglutamine) and that, from the molecular point of view, they are distinct from the SLIs in ALS.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004010051171
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