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  • Chloroplast ontogenesis  (2)
  • Aesculus hippocastanum  (1)
  • Chloroplast senescence  (1)
  • 1
    ISSN: 1615-6102
    Keywords: Aesculus hippocastanum ; Callus cultures ; Callus ultrastructure ; Embryogenic callus ; Non-embryogenic callus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cell ultrastructure in three types of callus obtained from leaf explants ofAesculus hippocastanum L. has been studied. Remarkable differences have been shown between the cells of the forerunner E1 callus and those of the callus arising from it, according to the culture conditions. The peculiar characteristics of E1 are the scarcity of intercellular spaces and the occurrence of autophagic vacuoles in the cells. An embryogenic friable callus (E2) is formed in time when E1 is maintained on solid culture medium. The E2 cells show cytological features typical of a higher metabolic level and contain starch. Diffused middle lamella digestion leads to the detachment of small embryogenic cell aggregates consisting of vacuolated parenchymatous-like cells and small meristematic cells which may be regarded as embryoids initials. Shaking E1 in the same liquid medium and subsequent culture on solid medium lead to the differentiation of a non-embryogenic callus (NE), whose cells are very large and highly vacuolated, devoid of starch and with organelle-rich cytoplasm. The NE callus shows a high degree of growth, but does not attain embryogenic competence in time.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1615-6102
    Keywords: Chloroplast ontogenesis ; Prolamellar body ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chloroplast differentiation in relation to increasing leaf age has been investigated in maize plants exposed to continuous illumination. In the young leaves the proplastids differentiate into chloroplasts containing well organized grana as well as prolamellar bodies. In the older leaves, while plastids differentiate, the prolamellar bodies are no longer detectable. Chloroplast ability to build up prolamellar bodies does not seems so much a light dependent process as it is affected by cell differentiation rate.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1615-6102
    Keywords: Spinacia oleracea L. ; Chloroplast ontogenesis ; Chloroplast senescence ; Spinach chloroplasts ; Intrathylakoidal crystals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The entire vital cycle of chloroplasts was followed in spinach leaves, in order to determine the plastidial situations leading to the build-up of intrathylakoidal crystals. This kind of inclusion was noticed in the juvenile ontogenetic stages as well as in the senescent chloroplast stage, while intrathylakoidal crystals were not present in the mature organelles. The appearance of such crystals, which are known to consist of ribulose 1,5-diphosphate carboxylase, is interpreted as a consequence of an imbalance between enzyme level and membrane function.
    Type of Medium: Electronic Resource
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