ISSN:
1432-1424
Keywords:
Rat jejunum
;
Basolateral membrane vesicles
;
Cl/HCO3 exchange
;
Na effect
;
Allosteric modifier site
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract A Cl/HCO3 exchanger mediates HCO3 extrusion across rat jejunal basolateral membrane. Previous studies demonstrated that anion antiport activity is positively affected by Na, but evidence was given that this cation is not translocated by the carrier protein. Basolateral membranes isolated from rat jejunum were used to give more insight on Na effect. Uptake studies, performed together with vesicle sidedness determinations, indicated that the greatest stimulation of Cl-dependent HCO3 uptake occurs when Na is present at both vesicle surfaces. The kinetic dependence of Cl/HCO3 exchange on equal intra- and extravesicular Na concentration showed a hyperbolic relationship, and the calculated kinetic parameters were V max=0.153 ± 0.006 nmol mg protein-1 sec-1, K m =23.0 Mm. Ion replacement studies indicated that Na can be partially substituted only by Li and not by other monovalent cations. Results of this study suggest that Na could act as a nonessential activator of the Cl/HCO3 exchanger. A possible role of the Na-sensitive modifier site in the physiology of jejunal enterocyte is suggested.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00211068
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